Abstract: Fruit bodies of freshly harvested Tricholoma giganteum strains SCAU1,Xin3 and Xin4 were used as experimental materials and stored at 12℃ with relative humidity of 95%,and the browning degree,polyphenol oxidase(PPO),tyrosine(TYR),laccase and total phenol were measured regularly,the correlation between browning degree and enzyme activity of each strain was investiged. The results showed that the browning degree of three strains was increased,the activity of PPO,TYR and laccase showed the downward trend. The browning degree,PPO,TYR and laccase activities of SCAU1 strain were lower than xin3 and xin4 strains,but the total phenol content in the three was hot significantly different(p>0.05);correlation analysis showed that the browning degree of xin 3 and xin 4 strains was extremely significantly negatively correlated with PPO activity(p<0.01),the browning degrce of SCAU1 strain was significantly negatively correcolted with PPO activity(p<0.05),the correlation from high to low was the Xin3,Xin4,SCAU1,and the browning degree of Xin3 strain was also significantly negatively correlated with the activity of TYR(p<0.05). This results could provide some theoretical guidance for the breeding of excellent strains of Tricholoma giganteum and revealing the intrinsic mechanism of browning.