Mitochondrial Protection of Stichopus japonicus Crude Polysaccharide and Its Underlying Mechanism
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摘要: 通过Fe2+/VC系统诱发肝线粒体脂质过氧化,采用硫代巴比妥酸显色法测定刺参粗多糖(Stichopus japonicuscrude polysaccharide,SJCP)对丙二醛(MDA)含量的影响;并测定SJCP对Fe2+的螯合作用、三种主要活性氧(ROS)(超氧阴离子(O2-·)、羟自由基(·OH)、过氧化氢(H2 O2))的清除作用和对还原力的影响。结果表明SJCP对丙二醛含量的升高有极显著的抑制作用(p < 0.01);当SJCP浓度为0.333 mg/mL时,其螯合率才达到30.86%,远不如EDTA组;随着SJCP浓度的升高,其对线粒体O2-·、·OH、H2 O2三种主要ROS的清除率也逐渐增大,还原力也随剂量的增加而增强,但弱于BHT组。说明SJCP能在一定程度上抑制线粒体发生脂质过氧化;具有一定的Fe2+螯合能力;能通过温和的抗氧化作用及清除ROS作用来保护线粒体,从而维持机体的健康。Abstract: In the experiment, lipid peroxidation of liver mitochondria was induced by Fe2+/Vitamin C in vitro and thiobarbituric acid (TBA) colorimetry was used to measure the effects of Stichopus japonicus crude polysaccharide (SJCP) on the content of malondialdehyde (MDA). And the chelating of Fe2+, the scavenging effects on reactive oxygen species (ROS) ((superoxide anion) O2-·, (hydroxyl radical)·OH, (hydrogen peroxide) H2 O2) and reducing power of SJCP were also determined.The results showed that SJCP had a very significant inhibitory effect on the increase of MDA (p < 0.01).When the concentration of SJCP was 0.333 mg/mL, the chelation rate reached 30.86%, and far less than the EDTA group. With the increase of SJCP concentration, the scavenging rate to three major mitochondrial ROS O2-·,·OH, H2 O2 were also gradually increased, and the reducing power was increased with the increase of dose, but weaker than the BHT group.It was indicated that SJCP could inhibit the lipid peroxidation of mitochondria to a certain extent, it had weak chelating ability to Fe2+, which indicated that SJCP could protect mitochondria through the mild anti-oxidation and removing ROS, so as to maintain the health of the body.
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