摘要:
本文建立了一测多评法(QAMS)测定藜麦中6种酚类成分的含量,并且对5种藜麦中6种酚类物质进行了比较。采用超高效液相色谱法,ACQUITYUPLC®BEHC18(2.1 mm×50 mm,1.7 μm)色谱柱,甲醇-0.2%冰乙酸水溶液为流动相,进行梯度洗脱,检测波长247 nm,流速0.2 mL/min,柱温35 ℃;以异荭草素为内参物,建立其与咖啡酸、对香豆酸、阿魏酸、芦丁、槲皮素的相对校正因子,采用一测多评法和外标法(ESM)同时计算各待测成分的含量,用SPSS软件对一测多评法和外标法所得含量进行分析。结果表明,5种藜麦品种中咖啡酸、对香豆酸、阿魏酸、异荭草素、芦丁、槲皮素的含量可用一测多评法进行测定,两种方法得到的含量值之间没有显著性差异(p>0.05);其中异荭草素、咖啡酸、对香豆酸、阿魏酸、芦丁、槲皮素在5种藜麦中的含量分别为210.34~370.61、3.77~8.04、168.86~300.21、10.98~17.15、31.13~42.75 μg/g。本文建立的以异荭草素为内标的一测多评法操作简单,重复性好,可为藜麦的质量评价提供参考。
Abstract:
A quantitative method of analysis of multi-components by single marker (QAMS) method for the quantification of six polyphenols in quinoa was established, and 6 polyphenols in 5 kinds of quinoa were compared. Samples were analyzed by ultra performance liquid chromatography (UPLC) system, equipped with a ACQUITYUPLC®BEHC18 (2.1 mm×50 mm, 1.7 μm) chromatographic column, with the mobile phase of methanol-0.2% glacial acetic acid solution at a flow rate of 0.2 mL/min. The column temperature and the detection wavelength were set at 35℃ and 247 nm respectively. The isoorientin was used as the internal reference to determine the relative correction factors (RCFs) of other five components, and their contents were calculated by their RCFs at the same time, and then compared with the external standard method (ESM) by SPSS software. No significant differences were found in the quantitative results of six polyphenols in 5 varieties of quinoa determined by ESM and QAMS (p>0.05). The contents of isoorientin, caffeic acid, p-coumaric acid, ferulic acid, rutin and quercetin in the five quinoa species were 210.34~370.61, 3.77~8.04, 168.86~300.21, 10.98~17.15, 31.13~42.75 μg/g, respectively. It was feasible and suitable to evaluate the quality of quinoa by QAMS with isoorientin as the internal reference.
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