Abstract: In this study,microwave assisted extraction(MAE) was firstly employed to extract amentoflavone from Selaginella doederleinii by response surface method. The effect of factors on the yield of amentoflavone was investigated, including microwave power,extraction time,solid-liquid ratio and extraction temperature. The response surface experiment design and multiple quadratic regression analysis were ordered to optimize the extraction technology. The results showed that the best extraction conditions were as follows:solid-liquid ratio 1:15 g/mL,microwave power 400 W,temperature 47℃,microwave extraction time 37 min. Under this condition, amentoflavone yield reached 0.33%±0.08%. Compared with ethanol reflux extraction and soxhlet extraction,MAE was not only increased the yield of target,but also reduced extraction time.The method is simple,accurate and efficient for the extraction of amentoflavone from Selaginella doederleinii.