猪血红蛋白超声提取工艺响应面优化及超声对其结构影响的研究

马素敏; 何立超; 李成梁; 孙媛; 靳国锋; 马美湖

doi:10.13386/j.issn1002-0306.2018.06.034

猪血红蛋白超声提取工艺响应面优化及超声对其结构影响的研究

1. 华中农业大学食品科技学院, 湖北武汉 430070;
2. 武汉设计工程学院食品与生物科技学院, 湖北武汉 430205

基金项目:

湖北省重大科技创新项目(2014ABA029)。

详细信息

作者简介:
马素敏(1992-),女,硕士研究生,研究方向:农产品加工及贮藏工程,E-mail:masu5200@163.com。

通讯作者:
靳国锋(1983-),男,博士,副教授,研究方向:畜产品加工及质量控制,E-mail:jgf@mail.hzau.edu.cn。

中图分类号: TS251.93
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出版历程
- 收稿日期: 2017-08-17
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-03-14

The response surface optimization on ultrasonic extraction process of porcine hemoglobin and effect of ultrasonic process on its structure

1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. College of Food and Biotechnology, Wuhan Institute of Design and Sciences, Wuhan 430205, China

摘要

摘要: 本文研究了超声破碎猪血红细胞提取血红蛋白的工艺,以血红蛋白提取率为优化指标,通过单因素实验研究了超声振幅、超声时间、间歇与脉冲激发时间比、水与红细胞体积比对血红蛋白提取率的影响。利用Box-Behnken响应面设计方法优化超声提取血红蛋白的最佳工艺为:超声振幅为26%,超声时间为10.15 min,间歇与脉冲激发时间比为2:1,水与红细胞体积比为4.25:1,此时血红蛋白提取率为91.4763%。此外,还利用紫外光谱、红外光谱、圆二色谱、显微共焦激光拉曼光谱方法研究分析了超声提取工艺对血红蛋白结构(包括卟啉环)的影响,结果发现超声辅助提取工艺可以获得较高的提取率,但是会对血红蛋白二级结构产生一定影响,导致血红素中心从疏水空穴暴露出来,卟啉环结构仍保持完整。本研究确定了最佳猪血红蛋白超声提取工艺,并能为后续卟啉类化合物制备及结构研究提供一定依据。
- 猪红细胞 /
- 血红蛋白 /
- 间歇式超声工艺 /
- 结构表征
Abstract: The ultrasonic extraction of hemoglobin from porcine blood was studied in this paper. With the yield of hemoglobin from red blood cell as evaluation index,the influence of amplitude and time of ultrasonic,ratio of quiescent intexal to impuse excitation time and volume ratio of water to red blood cell on the extraction process of hemoglobin were investigated by single-factor tests. The optimum extraction conditions were obtained through the Box-Behnken response surface:Ultrasonic amplitude of 26%,ultrasonic time of 10.15 min,quiescent interval to impulse excitation time ratio of 2∶1,water to red blood cell ratio of 4.25∶1.On this optimum extraction process conditions,the hemoglobin yield reached 91.4763%. In addition,the effect of ultrasonic process on hemoglobin structure was also investigated. The spectrum of UV-Vis,FTIR,Confocal Micro-Raman and circular dichroism were used to research the change of hemoglobin structure especially the structure of porphyrin ring. The main conclusion derived from this study was that ultrasonic extraction had an influence on the secondary structure of hemoglobin,it could cause the exposure of heme center from hydrophobic pocket,but the porphyrin rings remain intact. This research provided the optimal extraction process of porcine hemoglobin and could provide the theory basis for the further study of preparation and structural research of porphyrins compounds.
- porcine red blood cell /
- hemoglobin /
- intermittent ultrasonic extraction /
- structural characteristics

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参考文献(1)

[1]	Processing Technology

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期刊类型引用(5)

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