溶氧对耶氏解脂酵母油脂积累和柠檬酸分泌的影响

亢新鑫; 杨俊换; 张怀渊; 宋元达

doi:10.13386/j.issn1002-0306.2018.06.022

溶氧对耶氏解脂酵母油脂积累和柠檬酸分泌的影响

山东理工大学农业工程与食品科学学院, 山东淄博 255000

基金项目:

国家自然科学基金(31670064)。

详细信息

作者简介:
亢新鑫(1991-),女,硕士研究生,研究方向:微生物油脂,E-mail:15169352965@163.com。

通讯作者:
宋元达(1964-),男,博士,教授,研究方向:微生物油脂的生物合成,E-mail:ysong@sdut.edu.cn。

中图分类号: TS201.3
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出版历程
- 收稿日期: 2017-08-24
- 网络出版日期: 2020-11-23
- 刊出日期: 2018-03-14

Influence of dissolved oxygen on accumulation of lipid and secretion of citric acid by Yarrowia lipolytica fermentation

School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, China

摘要

摘要: 产油耶氏解脂酵母能将培养基中过量的碳源转化为油脂储存于细胞内并分泌大量的柠檬酸。在耶氏解脂酵母发酵过程中通过关联搅拌转速和通气量调控培养基中的溶氧含量处于5%、10%、20%、30%和不控制5种水平,来研究溶氧对其油脂积累和柠檬酸分泌的影响。结果表明,随着发酵培养基中溶氧的增加,耶氏解脂酵母细胞内油脂含量和柠檬酸分泌量均有所增加,且不控制培养基中溶氧时,细胞内油脂含量和柠檬酸产量均最高,油脂含量达到细胞干重的11.62%(w/w),柠檬酸产量达到21.0 g/L。培养基中不同的溶氧含量还会影响油脂的脂肪酸组成,高溶氧能够促进油酸含量的增加,溶氧不控制时油酸的含量最高,达到48.62%。本研究为耶氏解脂酵母产脂发酵培养过程中溶氧的控制提供了重要的实验依据。
- 溶氧 /
- 耶氏解脂酵母 /
- 微生物油脂 /
- 柠檬酸
Abstract: Among oleaginous microorganisms,Yarrowia lipolytica can convert excess carbon source from the medium into lipids stored in the cells and secrete large amounts of citric acid.Dissolved oxygen(DO)concentration in the culture medium during fermentation process was controlled by stirring speed and ventilation which was selected for five levels i.e.:5%,10%,20%,30% and uncontrol.The results indicated that the increase of dissolved oxygen resulted in an increased production of lipid and citric acid of Yarrowia lipolytica.However,the maximum yield of lipid and citric acid was achieved by normal fermentation without controlling of dissolved oxygen concentration. The obtained lipid content and citric acid concentration were 11.62%(w/w)and 21.0 g/L respectively.Furthermore,dissolved oxygen concentration can also affect the lipid fatty acid composition.High dissolved oxygen could promote the increase of oleic acid content,and the highest content of oleic acid was 48.62% which was obtained in the uncontrol level. This study would provide an important basis for the control of dissolved oxygen concentration in lipid accumulation by Yarrowia lipolytica.
- dissolved oxygen /
- Yarrowia lipolytica /
- microbial oil /
- citric acid

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[1]	Bioengineering

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