群体感应系统及其在乳酸菌生物膜形成中的作用

邬慧颖; 韩雪; 张丽娟; 王玥

doi:10.13386/j.issn1002-0306.2018.01.058

群体感应系统及其在乳酸菌生物膜形成中的作用

哈尔滨工业大学化工与化学学院, 黑龙江哈尔滨 150090

详细信息

作者简介:
邬慧颖(1994-),女,硕士研究生,研究方向:乳品微生物,E-mail:1508648992@qq.com。

通讯作者:
韩雪(1978-),女,博士,副教授,研究方向:乳品微生物,E-mail:xhan@hit.edu.cn。

中图分类号: TS252.1
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出版历程
- 收稿日期: 2017-03-23
- 网络出版日期: 2020-12-28
- 刊出日期: 2017-12-31

Quorum sensing system and its role in regulation of biofilm formation in lactic acid bacteria

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China

摘要

摘要: 以黑果枸杞花青素微胶囊在水相体系中花青素的保留率为指标,采用控制变量法研究了环境因素温度、循环冻融、自然光照、紫外光照对花青素稳定性的影响,进一步研究了花青素的氧化降解规律;采用体外模拟胃肠液的方法对花青素微胶囊的稳定性和靶向释放特性进行了研究。结果表明:花青素微胶囊的热降解符合一级降解动力学模型;微胶囊化花青素在低温和低 pH条件下较稳定,光照能够降低花青素的稳定性。在体外模拟胃肠液稳定性实验中,花青素微胶囊在胃液中比肠液中稳定,与未包埋花青素溶液相比,花青素微胶囊溶液在模拟胃液中其花青素的保留率提高了14.8%,在模拟肠液中提高了17.3%;在体外模拟胃肠液靶向释放实验中,在胃液中,90 min 后,花青素微胶囊溶液和花青素未包埋溶液中花青素的释放率分别为7.5%、20.7%;在肠液中花青素缓慢释放,240 min 后,二者花青素的释放率分别为21.9%、27.2%;并且花青素微胶囊后能够显著提高其在水相体系中的稳定性,起到在胃肠液中缓释的效果。
- 群体感应 /
- 信号分子 /
- 乳酸菌 /
- 生物膜
Abstract: Quorum sensing(QS)is a behavior model in which bacteria rely on cell density for information transfer. In lactic acid bacteria(LAB), the formation of biofilm was regulated by QS. This article reviewed the component, the signal transduction mechanism and the regulation of QS system in biofilm formation of LAB, in order to provide new ideas for improving the stability of LAB.
- quorum sensing /
- signal molecules /
- lactic acid bacteria /
- biofilm

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