糖基化方法对于大豆7S球蛋白糖基化反应及产物的影响

许彩虹; 王金梅; 杨晓泉; 于淑娟

doi:10.13386/j.issn1002-0306.2018.01.004

糖基化方法对于大豆7S球蛋白糖基化反应及产物的影响

1. 广东食品药品职业学院食品学院, 广东广州 510220;
2. 华南理工大学食品科学与工程学院, 淀粉与植物蛋白深加工教育部工程研究中心, 广东广州 510640

基金项目:

广东省科技计划项目(2014B020205001)。

中央高校基本科研业务费项目(SCUT,2015ZZ115)

详细信息

作者简介:
许彩虹(1976-)女,博士,讲师,研究方向:碳水化合物和蛋白质改性方向,E-mail:chx207@sohu.com。

中图分类号: TS201.1
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出版历程
- 收稿日期: 2017-06-19
- 网络出版日期: 2020-12-28
- 刊出日期: 2017-12-31

Effects of glycosylation methods on glycation reaction and products of soybean 7S globulin

1. School of Food Science, Guangdong Food and Drug Vocational College, Guangzhou 510220, China;
2. Research and Development Center of Food Proteins, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China

摘要

摘要: 针对传统的两种糖基化接枝方法和改进方法从糖基化反应程度进行系统比较,并对产物氨基酸组成进行分析,以期进一步揭示蛋白质糖基化方法对于糖基化反应过程及产物的影响机制。本文利用干热法、湿热法和加压辅助湿热三种糖基化方法,对大豆7S球蛋白和葡聚糖的糖基化产物从反应程度、氨基酸组成等方面进行研究发现,结果表明:三种制备方法的反应程度高低顺序依次为:湿热法>加压辅助湿热法>干热法,说明压力能够对Maillard反应的进行具有一定的抑制作用,可以控制反应向理想阶段进行。大豆7S球蛋白和葡聚糖的糖基化主要发生在蛋白质肽链上的赖氨酸和精氨酸侧链上的自由氨基,干热法与其它两种方法相比糖链更有易于和精氨酸侧链上的自由氨基发生共价交联,而湿热法和加压辅助湿热法相比糖链更易于和赖氨酸侧链上的自由氨基发生共价交联。
- 糖基化 /
- 大豆7S球蛋白 /
- 葡聚糖 /
- 压力
Abstract: The two traditional glycosylation grafting methods and the improved methods were used to compare with the glycosylation degree systemand the product of amino acid composition analysis, in order to further reveal the effect of protein glycosylation grafting methods on the process of glycosylation reaction and the product mechanism. In this paper, the glycosylation products of soybean 7S globulin and dextran were studied from three aspects:reaction degree and amino acid composition and other aspects by three methods:the dry heat method, the liquid heat method and the hydrothermal method.The results showed, the degree of reaction was:the liquid heat method>the hydrothermal method>the dry heat method, it was showed that the pressure can inhibit the Maillard reaction and control the reaction to the ideal stage. Glycosylation of soybean 7S globulin and dextran occurred predominantly in the free amino groups of the lysine and arginine side chains on the protein peptide chain. it was indicated that sugar chain for dry method was easy to covalently cross-linked with the free amino groups on the arginine, whereas sugar chain for the liquid heat method and the hydrothermal method were more easily to covalently cross the free amino groups than the lysine side chains.
- glycosylation /
- soybean 7S globulin /
- dextran /
- pressure

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参考文献(26)

[1]	Lagemaat J,Silván MJ,MorenoJF,et al. In vitro glycation and antigenicity of soy proteins[J]. Food research international,2007,40:153-160.
[2]	BellLN. Kinetics of non-enzymatic browning in amorphous solid systems:distinguishing the effects of water activity and the glass transition[J]. Food research international,1995,28:591-597.
[3]	Wooster TJ,Augustin MA.β-Lactoglobulin-dextran Maillard conjugates:Their effect on interfacial thickness and emulsion stability. Journal of Colloid and Interface Science,2006,303:564-572.
[4]	Medrano A,Abirached C,Panizzolo L,et al. The effect of glycation on foam and structural properties of β-lactoglobulin[J].Food Chemistry,2009,113:127-133.
[5]	Wang J,Sun BG,Cao YP,et al. Protein glycation inhibitory activity of wheat bran feruloyl oligosaccharides[J]. Food Chemistry,2009,112:350-353.
[6]	Dickinson E,Mcclements DJ. Adbances in food colloids[M]. Blackic Academic and Professional. 1996.
[7]	Zhu D,Damodaran S,Lucey JA. Physicochemical and emulsifying properties of whey protein isolate (WPI)-dextran conjugates produced in aqueous solution[J]. Journal of Agricultural and Food Chemistry,2010,58:2988-2994.
[8]	Petueccelli S,Anon MC. Thermal aggregation of soy protein isolates[J]. Journal of Agricultural and Food Chemistry,1995,43:3035-3041.
[9]	Lertittikul W,Benjakul S,Tanaka M. Characteristics and antioxidative activity of Maillard reaction products from a porcine plasma protein-glucos model system as influenced by pH[J]. Food Chemistry,2007,100:669-677.
[10]	Xi Zhang,Jun-Qu Qi,Kang-Kang LI,et al. Characterization of soy β-conglycinin-dextran prepared by Maillard reaction in crowed liquid system[J]. Food Reseach International,2012,49:648-654.
[11]	Guan JJ,Qiu AY,Liu XY,et al. Microwave improvement of soy protein isolate-saccharide graft reactions[J]. Food Chemistry,2006,97:577-585.
[12]	齐军茹.蛋白-多糖共价复合物的制备及其功能性质的研究[D].广州:华南理工大学博士论文,2004.
[13]	Yong-GuangGuan,Hua Lin,Zhong Han,Jun Wang,et al. Effects of pulsed electric field treatment on a bovine serum albumin-dextran model system,a means of promoting the Maillard reaction. Food Chemistry. 2010.123:275-280.
[14]	Bo Zhang,Yujie JC,Bing Li. Effect of ultrasound treatment on the wet heating Maillard reaction between β-conglycinin and maltodextrin and on the emulsifying properties of conjugates. European Food Research and Technology,2014,238:129-138.
[15]	Nagano T,Hirotsuka M,Mori H,et al. Dynamic Viscoelastic Study on the Gelation of 7S Globulin from Soybeans[J]. Journal of Agricultural and Food Chemistry,1992,40:941-944.
[16]	Cai-hong Xu,Xiao-quan Yang,Shu-juan Yu,et al. Emulsifying properties and structural characteristics of β-conglycinin and dextran conjugates synthesized in a pressurized liquid system[J].International Journal of Food Science&Technology. 2010.45:995-1001.
[17]	Kato A,Minaki K,Kobayashi K. Improvement of emulsifying properties of egg white proteins by the attachment of polysaccharide through Maillard reaction in a dry state[J]. Journal of Agricultural and Food Chemistry,1993,41:540-543.
[18]	Cai-hong Xu,Xiao-quan Yang,Shu-juan Yu,et al. The effect of glycosylation with dextran chains of differing lengths on the thermal aggregation of β-conglycinin and glycinin[J]. Food Research International. 2010.43:2270-2276.
[19]	Baisier WM,Labuza TP. Maillard browning kinetics in a liquid model system[J]. Journal of Agricultural and Food Chemistry,1992,40(5):707-713.
[20]	Laroque D,Inisan C,Berger C,et al. Kinetic study on the Maillard reaction. Consideration of sugar reactivity[J]. Food Chemistry,2008,111:1032-1042.
[21]	Morales FJ,Salvio FP. Free radical scavenging capacity of Maillard reaction products as related to colour and fluorescence[J]. Food Chemistry,2001,72:119-125.
[22]	Tamaoka T,Itoh N. HayashiR. High pressure effect on Maillard reaction[J]. Agricultural and Biological Chemistry,1991,55(8):2071-2074.
[23]	Isaacs NS,Coulson M. Effect of Pressure on processes modeling the Maillard reaction[J]. Journal of physical organic chemistry,1996,9:639-644.
[24]	Tang C H,Chen L,Ma CY. Effect of high pressure treatment on aggregation and structural properties of soy protein isolate[J]. Swiss Society of Food Science and Technology,2009,42:606-611.
[25]	Jing H,Kitts DD. Chemical and biochemical properties of casein-sugar Maillard reaction products[J]. Food and Chemical Toxicology. 2002,40:1007-1015.
[26]	Morales FJ,Boekel V MAJS. A study on advanced Maillard reaction in heated casein/sugar solutions:fluorescence accumulation[J]. International Dairy Journal,1997,7:675-683.