超声辅助溶剂棒微萃取-液相色谱法快速测定婴儿配方奶粉中的双酚A

王力; 刘姝妤; 赵雯; 陈百玲; 潘家荣

doi:10.13386/j.issn1002-0306.2017.24.048

超声辅助溶剂棒微萃取-液相色谱法快速测定婴儿配方奶粉中的双酚A

三峡大学生物与制药学院

基金项目:

湖北省教育厅科研项目(B2015256)；宜昌市科技局自然基础科学研究与应用专项(A15-302-a05)；三峡大学科学基金项目(KJ2013B070)；

详细信息

作者简介:
王力 (1982-) , 女, 博士, 讲师, 研究方向:食品分析化学, E-mail:wangli@ctgu.edu.cn。;

中图分类号: O657.72;TS252.51
计量
- 文章访问数: 199
- HTML全文浏览量: 31
- PDF下载量: 89
出版历程
- 收稿日期: 2017-07-27

Rapid determination of bisphenol A in infant formula milk powder by ultrasound-assisted solvent bar microextraction coupled with high performance liquid chromatography

College of Biological and Pharmaceutical Science, China Three Gorges University

摘要

摘要: 建立超声辅助溶剂棒微萃取-高效液相色谱（UA-SBME-HPLC）方法,用于快速测定婴儿配方奶粉中的双酚A（BPA）。设计超声辅助溶剂棒微萃取系统,优化后的最佳萃取条件为:溶剂棒的有效长度为30 mm,萃取溶剂为正辛醇,搅拌速度为600 r/min,超声时间10 min,萃取时间15 min,pH=6的HCl溶液为给体相,0.12 mol/L NaOH溶液为受体相,NaCl的加入浓度为60 g/L。在最优萃取条件下,该方法对BPA的平均富集倍数为102倍,方法的线性范围为0.1¹00μg/L（r=0.9989）,检出限为0.35μg/L（S/N=3）,相对标准偏差RSD小于4.32%（n=6）,加标回收率为89.7%¹02.8%。研究表明该方法绿色环保,可用于准确快速测定婴儿配方奶粉中的双酚A。
- 双酚A /
- 婴儿配方奶粉 /
- 超声辅助溶剂棒微萃取（UA-SBME） /
- 高效液相色谱(HPLC)
Abstract: A technique for the rapid determination of bisphenol-A ( BPA) in infant formula milk powder was developed by ultrasound-assisted solvent bar microextraction coupled with high performance liquid chromatography.The system of ultrasound-assisted solvent bar microextraction was designed. The optimized microextraction conditions were as follows: the length of solvent bar was 30 mm, 1-octanol was used as the extraction solvent, the stirring rate was 600 r/min, the ultrasonic time was 10 min and the extraction time was 15 min, the pH of sample solutions was 6, 0.12 mol/L of NaOH was used as the acceptor phase, the concentration of NaCl was 60 g/L. Under the optimal microextraction conditions, the average enrichment factor was102, the linear range was 0.1 ~ 100 μg/L ( r = 0.9989) , the limits of detection was 0.35 μg/L ( S/N = 3) , and the relative standard deviations ( RSD) was lower than 4.32% ( n = 6) . The proposed method was applied to the determination of BPA in infant formula milk powder samples.The spiked recoveries were in the range of 89.7% ¹02.8%.It was showed that the proposed method was environmentally for the rapid determination of BPA in infant formula milk powder.
- bisphenol A (BPA) /
- infant formula milk powder /
- ultrasound-assisted solvent bar microextraction (UA-SBME) /
- high performance liquid chromatography (HPLC)

HTML全文

参考文献(33)

[1]	Wang X, Zeng H L, Zhao L X, et al.Selective determination of bisphenol A (BPA) in water by a reversible fluorescence sensor using pyrene/dimethyl beta-cyclodextrin complex[J].Analytica Chimica Acta, 2006, 556 (2) :313-318.
[2]	Yin H, Zhou Y, Ai S, et al.Sensitivity and selectivity determination of BPA in real water samples using PAMAM dendrimer and Co Te quantum dots modified glassy carbon electrode[J].Journal of Hazardous Materials, 2010, 174 (1-3) :236-243.
[3]	Zimmers S M, Browne E P, O’Keefe P, et al.Determination of free bisphenol A (BPA) concentrations in breast milk of US women using a sensitive LC/MS/MS method[J].Cancer Research, 2013, 73 (8) :10.
[4]	高向阳, 王长青.流动注射抑制化学发光法快速测定婴幼儿奶瓶中的双酚A[J].食品科学, 2012, 33 (8) :184-187.
[5]	Pandelova M, Piccinelli R, Lopez W L, et al.Assessment of PCDD/F, PCB, OCP and BPA dietary exposure of non-breast-fed European infants[J].Food Additives and Contaminants Part a-Chemistry Analysis Control Exposure and Risk Assessment, 2011, 28 (8) :1110-1122.
[6]	Hatwell K R, Mihalov J J, Bailey A B.Assessing exposure to BPA for infants, toddlers, and adults[J].Abstracts of Papers of the American Chemical Society, 2012, 244:140-142.
[7]	曲双双, 肖光辉, 刘婷, 等.UPLC-MS/MS法同时测定婴幼儿配方奶粉中的壬基酚和双酚A[J].中国食品添加剂, 2016 (8) :185-190.
[8]	丘秀珍, 黄志伟, 朱惠娟, 等.分子印迹磁性固相萃取/液相色谱法检测奶制品中的双酚A[J].分析测试学报, 2017, 36 (2) :236-241.
[9]	Kubwabo C, Kosarac I, Lalonde K, et al.Quantitative determination of free and total bisphenol A in human urine using labeled BPA glucuronide and isotope dilution mass spectrometry[J].Analytical and Bioanalytical Chemistry, 2014, 406 (18) :4381-4392.
[10]	Zimmers S M, Browne E P, O’Keefe P W, et al.Determination of free Bisphenol A (BPA) concentrations in breast milk of US women using a sensitive LC/MS/MS method[J].Chemosphere, 2014, 104:237-243.
[11]	Gallo P, Di Marco Pisciottano I, Esposito F, et al.Determination of BPA, BPB, BPF, BADGE and BFDGE in canned energy drinks by molecularly imprinted polymer cleaning up and UPLC with fluorescence detection[J].Food Chemistry, 2017, 220:406-412.
[12]	田泉, 刘英丽, 王静, 等.高效液相色谱法测定PC奶瓶中双酚A的含量及其迁移量[J].食品科学, 2012, 33 (22) :255-258.
[13]	白玮玮, 刘书慧, 曹江平, 等.亚临界水萃取-高效液相色谱法测定聚碳酸酯水杯中双酚A和苯酚的迁移量[J].色谱, 2013, 31 (3) :254-259.
[14]	胡文凌, 张杰, 余卫娟, 等.磁固相萃取-高效液相色谱法测定环境水样中双酚A[J].理化检验-化学分册, 2015, 51 (2) :153-156.
[15]	刁春鹏, 李聪, 彭辉, 等.基于磁性多壁碳纳米管的涡旋辅助分散基质固相萃取-高效液相色谱检测水中双酚A[J].中国环境监测, 2016, 32 (5) :111-115.
[16]	吴新华, 丁利, 肖家勇, 等.单分散磁性亚微米粒子固相萃取-液相色谱-串联质谱法测定牛奶中的双酚A[J].色谱, 2011, 29 (5) :399-403.
[17]	马晓斐, 宋炜, 梁天佐, 等.微波辅助萃取-超高效液相色谱-质谱法测定食品用纸容器中双酚A[J].食品科学, 2014, 35 (16) :165-169.
[18]	Austin J F, Przyjazny A, Kokosa J M.Application of liquidphase microextraction to environmental analysis[J].Abstracts of Papers of the American Chemical Society, 1999, 217:458-459.
[19]	Hyder M, Genberg J, Jonsson J A.Application of hollow fiber liquid phase microextraction for pinic acid and pinonic acid analysis from organic aerosols[J].Analytica Chimica Acta, 2012, 713:79-85.
[20]	Shrivas K, Patel D K.Ultrasound assisted-hollow fibre liquid-phase microextraction for the determination of selenium in vegetable and fruit samples by using GF-AAS[J].Food Chemistry, 2011, 124 (4) :1673-1677.
[21]	谭小旺, 宋燕西, 魏瑞萍, 等.三相中空纤维膜液相微萃取-高效液相色谱法测定水中痕量双酚A[J].分析化学, 2012, 40 (9) :1409-1414.
[22]	康明芹, 李玲, 尚鸿民, 等.离子液体均相液液微萃取-高效液相色谱法测定水中痕量双酚A[J].分析实验室, 2017, 36 (5) :530-533.
[23]	Jiang X M, Lee H K.Solvent bar microextraction[J].Analytical Chemistry, 2004, 76 (18) :5591-5596.
[24]	Bandforuzi S R, Hadjmohammadi M R.Solvent bar microextraction using a reverse micelle containing extraction phase for the determination of warfarin from human plasma by highperformance liquid chromatography[J].Journal of Chromatography A, 2017, 1496:1-8.
[25]	Wu L, Song Y, Hu M, et al.Application of magnetic solvent bar liquid-phase microextraction for determination of organophosphorus pesticides in fruit juice samples by gas chromatography mass spectrometry[J].Food Chemistry, 2015, 176:197-204.
[26]	Wang L, Wang L, Chen J, et al.Ultrasonic-assisted water extraction and solvent bar microextraction followed by gas chromatography ion trap mass spectrometry for determination of chlorobenzenes in soil samples[J].Journal of Chromatography A, 2012, 1256:9-14.
[27]	Ghasemi J B, Zolfonoun E.Ultrasound-assisted ionic liquidbased microextraction combined with least squares support vector machines regression for the simultaneous determination of aluminum, gallium, and indium in water and coal samples[J].Environmental Monitoring and Assessment, 2012, 184 (6) :3971-3981.
[28]	Yan H Y, Liu B M, Du J J, et al.Ultrasound-assisted dispersive liquid-liquid microextraction for the determination of six pyrethroids in river water[J].Journal of Chromatography A, 2010, 1217 (32) :5152-5157.
[29]	Yan H Y, Du J J, Zhang X G, et al.Ultrasound-assisted dispersive liquid-liquid microextraction coupled with capillary gas chromatography for simultaneous analysis of nine pyrethroids in domestic wastewaters[J].Journal of Separation Science, 2010, 33 (12) :1829-1835.
[30]	赵艳芳, 宋吉英, 徐鲁斌.超声辅助分散液相微萃取-高效液相色谱法测定牛奶中的双酚A[J].青岛农业大学学报:自然科学版, 2011, 28 (2) :154-156.
[31]	Pedersen-Bjergaard S, Rasmussen K E.Liquid-phase microextraction with porous hollow fibers, a miniaturized and highly flexible format for liquid-liquid extraction[J].Journal of Chromatography A, 2008, 1184 (1-2) :132-142.
[32]	Barri T, Jonsson J A.Advances and developments in membrane extraction for gas chromatography-Techniques and applications[J].Journal of Chromatography A, 2008, 1186 (1-2) :16-38.
[33]	Cui S F, Tan S, Ouyang G F, et al.Automated polyvinylidene difluoride hollow fiber liquid-phase microextraction of flunitrazepam in plasma and urine samples for gas chromatography/tandem mass spectrometry[J].Journal of Chromatography A, 2009, 1216 (12) :2241-2247.