牛初乳冻干粉和常乳冻干粉的降血糖及抗氧化作用

王小军; 高睿嘉; 郁桦; 狄怡琳; 李玲; 王艺茗; 丁宁; 魏扬智; 于天阳; 葛文金; 王荣昌; 程光宇

doi:10.13386/j.issn1002-0306.2017.23.048

牛初乳冻干粉和常乳冻干粉的降血糖及抗氧化作用

南京师范大学生命科学学院
江苏天美健大自然生物工程有限公司
南京师范大学中北学院

基金项目:

国家自然科学基金项目(31500373)；江苏省品牌专业支助(PPZY2015B117)；

详细信息

作者简介:
王小军 (1981-) , 男, 博士, 实验师, 研究方向:生物活性物质分离纯化及功能食品研究, E-mail:wangxiaojun0910@njnu.edu.cn。;

中图分类号: TS252.1
计量
- 文章访问数: 284
- HTML全文浏览量: 13
- PDF下载量: 248
出版历程
- 收稿日期: 2017-05-07

Hypoglycemic and antioxidation effect of lyophilized bovine colostrums and lyophilized milk

College of Life Sciences, Nanjing Normal University
Jiangsu TMG nature Biological Engineering Co., Ltd
Nanjing Normal University Zhongbei College

摘要

摘要: 目的:研究牛初乳冻干粉（LBC）和常乳冻干粉（LM）降血糖及抗氧化作用。方法:采用生化分析方法测定自由基清除活性及IGFs和胰岛素含量。建立小鼠四氧嘧啶糖尿病模型,分别给予降糖灵（100 mg/kg）、LM（600 mg/kg）和LBC（100 mg/kg、600 mg/kg）,以正常小鼠作对照。30 d后,测定血糖、糖耐量及抗氧化等指标。结果:LBC清除O₂^-·,DPPH·的EC₅₀值为245.3,167.2μg/m L,IGF-1、IGF-Ⅱ和胰岛素含量分别为1146.5、1303.0、128.4 ng/g。LM对羟自由基有一定清除作用,IGFs含量显著低于LBC,未检出胰岛素。LBC对糖尿病小鼠病症有明显改善;能显著降低血糖水平,降低血清MDA和TC含量;能提高糖耐量和血液SOD活性。LM对血糖水平和抗氧化指标无明显改善。结论:LBC比LM具有更高的降糖活性和自由基清除活性,对糖尿病小鼠有降血糖及抗氧化作用。
- 牛初乳冻干粉 /
- 常乳冻干粉 /
- 降血糖 /
- 类胰岛素生长因子 /
- 抗氧化作用 /
- 小鼠
Abstract: Objective:To study on hypoglycemic effect and antioxidative effects of lyophilized bovine colostrums (LBC) and lyophilized milk (LM) .Methods:The free radical scavenging activity, IGFs and insulin content were detected by biochemical analysis.Alloxan diabetes model was induced in mice where rats were treated with Phenformin (100 mg/kg) , LM (600 mg/kg) and LBC (100、600 mg/kg) respectively.Besides, normal mice were used as control.The blood glucose, glucose tolerance and antioxidant and other indicators were measured after 30 days.Results:The EC₅₀of scavenging superoxide free radicals and DPPH free radicals were respectively 245.3, 167.2μg/m L in LBC.The content of IGF-I, IGF-Ⅱand insulin of LBC were 1146.5, 1303.0, 128.4 ng/g respectively.LM had a part of scavenging effect on hydroxyl free radicals, and the content of IGFs was significantly lower than LBC and insulin was not detected.LBC made significantly improvement on diabetic mice, significantly lower blood glucose levels, reduced the content of MDA and TC in serum, enhanced the tolerance and blood SOD activity.LM had no significant improvement in blood glucose level and antioxidant index.Conclusion:LBC included higher hypoglycemic factors, producted exhibited strong radical-scavenging activity in vivo than LM, which has hypoglycemic and antioxidative effects on diabetic mice.
- lyophilized bovine colostrums /
- lyophilized milk /
- hypoglycemic effect /
- insulin-like growth factor /
- antioxidation /
- mice

HTML全文

参考文献(32)

[1]	曹劲松.初乳功能性食品[M].北京:中国轻工业出版社, 2000.
[2]	于立芹, 庞广昌.牛初乳中生物活性成分的研究进展[J].食品科学, 2007, 28 (9) :604-609.
[3]	张挺秀, 胡德芳, 蒋宁南, 等.牛初乳提取物 (BCE) 对儿童免疫功能影响的探讨[J].江苏卫生保健, 1999, 1 (3) :124-125.
[4]	徐丽, 生庆海, 郭顺堂.不同剂量牛初乳粉对小鼠免疫调节作用的研究[J].食品科学, 2005, 26 (11) :227-230.
[5]	江海涛, 吴京燕, 程光宇, 等.初乳素预防化学性肝损伤的实验研究[J].中国乳品工业, 2005, 33 (5) :12-14.
[6]	江海涛, 任源浩, 陈勇, 等.牛初乳冻干粉改善小鼠睡眠状况的实验研究[J].中国乳品工业, 2006, 34 (12) :29-30.
[7]	王晓炜, 程光宇, 吴京燕, 等.大豆异黄酮和牛初乳复合制剂对去卵巢大鼠骨密度及子宫组织抗氧化作用的研究[J].食品科学, 2007, 28 (2) :297-301.
[8]	张志新, 王颖明, 钟才云, 等.牛初乳素对促进小鼠神经系统发育的研究[J].中国畜产与食品, 1998, 5 (4) :150-152.
[9]	Kim S E, Ko I G, Shin M S, et al.Neuroprotective effects of bovine colostrum on intracerebral hemorrhage-induced apoptotic neuronal cell death in rats[J].Neural Regeneration Research, 2012, 7 (22) :1715-1721.
[10]	俞茂华, 朱禧星, 史虹莉, 等.牛初乳制剂治疗2型糖尿病的临床初步观察[J].上海医科大学学报, 1992, 19 (1) :41-44.
[11]	丁红, 王颢, 于亚鹭, 等.牛初乳粉对实验性糖尿病的作用[J].卫生研究, 2006, 35 (5) :579-580.
[12]	Nikolic I, Stojanovic I, Vujicic M, et al.Standardized bovine colostrum derivative impedes development of type 1 diabetes in rodents[J].Immunobiology, 2017, 222 (2) :272-279.
[13]	中华人民共和国卫生部.保健食品检验与评价技术规范[S], 2003.
[14]	Stewert R C, Bewley J D.Lipid peroxidation associated with accelerated aging of soybean axes[J].Plant Physiology, 1980, 65 (2) :245-248.
[15]	王峰, 王晓炜, 陶明煊, 等.大球盖菇多糖清除自由基活性和对D-半乳糖氧化损伤小鼠的抗氧化作用[J].食品科学, 2009, 30 (5) :233-238.
[16]	马晓华, 连宾.几种常见食用菌清除羟基自由基能力的研究[J].食品与发酵工业, 2005, 31 (10) :25-28.
[17]	Dasgupta N, Bratati D.Antioxidant activity of Piper betle L.leaf extract in vitro[J].Food chemistry, 2004, 88 (2) :219-224.
[18]	云振宇.牛初乳中IGF-I的分离及其降血糖作用研究[D].呼和浩特市:内蒙古农业大学, 2004.
[19]	崔福生.医学生化检验手册[M].天津:天津科学技术出版社, 1982.
[20]	Dubowski K M.An o-toluidine method for body-fluid glucose determination[J].Clinical Chemistry, 1962, 8 (3) :215-235.
[21]	Ollikainen P, Muuronen K.Determination of insulin-like growth factor-1 and bovine insulin in raw milk and its casein and whey fractions after microfiltration and ultrafiltration[J].International dairy Journal, 2013, 28 (2) :83-87
[22]	Prosser C G.Insulin-like growth factors in milk and mammary gland[J].Journal of mammary gland, biology and neoplasia, 1996, 1 (3) :297-306.
[23]	刘海燕, 潘道东.免疫初乳及过渡乳中类胰岛素生长因子-I含量测定及其变化[J].食品工业科技, 2009, 30 (2) :117-119.
[24]	云振宇, 张列兵, 张和平.牛初乳类胰岛素生长因子-I治疗大鼠高血糖作用及其机理的研究[J].中国食品学报, 2007, (5) :18-23.
[25]	云振宇, 张列兵, 张和平.牛初乳类胰岛素生长因子-I的降糖作用研究[J].中国乳品工业, 2006, 34 (2) :7-9.
[26]	孙侃, 俞茂华, 史虹莉, 等.牛初乳短链IGF-I对STZ糖尿病大鼠物质代谢及肾脏的影响[J].中华内分泌代谢杂志, 1997, 13 (3) :162-166.
[27]	张娟霞, 孙鹏, 杨永新, 等.牛奶中抗氧化物质研究进展[J].中国畜牧兽医, 2012, 39 (7) :142-146.
[28]	周之辉, 史奎雄, 潘碧霞, 等.牛初乳提取物 (BCE) 对老年人抗衰老作用的研究[J].营养学报, 1994, 16 (2) :169-173.
[29]	程光宇, 唐梓进, 吴京燕, 等.活性初乳素对小鼠的抗衰老作用[J].南京师大学报:自然科学版, 1996, 19 (3) :69-72.
[30]	马宁, 张北奇, 张硕立, 等.牛初乳对老年糖尿病患者及老年糖尿病大鼠保护作用的临床与实验研究[J].中国老年学杂志, 1995, 15 (2) :91-93.
[31]	王安平.牛初乳中类胰岛素生长因子-Ⅰ的分离及其对糖尿病预防作用研究[D].呼和浩特市:内蒙古农业大学, 2007.
[32]	Oda Shinnichi, Satoh Hidetoshi, Sugawara Tatsurou, et al.Insulin-like growth factor-I, GH, insulin and glucagon concentrations in bovine colostrum and in plasma of dairy cows and neonatal calves around parturition[J].Comparative Biochemistry and Physiology Part A:Physiology, 1989, 94 (4) :805-808.