Determination of organophosphorous pesticides in cider by GC-MS coupled with cloud point extraction
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摘要: 本文对苹果汁中45种有机磷类农药的提取、净化技术和气相色谱质谱检测条件进行了研究。以苹果汁为基质材料,45种有机磷类农药在1、5、10μg/L三个水平上对苹果汁进行添加回收率实验。结果表明:45种有机磷类目标物在优化条件下的回收率为71.16%~117.94%,相对标准偏差1.20%~15.13%(n=5),方法检出限为0.01~0.22μg/L。45种有机磷类农药能够实现很好的分离,线性范围在10~2000μg/L,线性相关系数大于0.984。本方法操作简单,结果准确,由于几乎不使用有机溶剂也减少了分析过程对环境和人体的危害。Abstract: This article focused on the technique using GC-MS to determine the presence and concentrations of 45 kinds of organophosphorous pesticides in cider. 45 pesticides were tested with the recovery experiment of cider at three levels: 1, 5, 10 μg/L. The results showed that the 45 pesticides gave satisfactory recoveries ranging from 71.16% to 117.94% with the RSDs from 1.20% to 15.13% ( n = 5) , the limitation of this method was between 0.01 μg/L and 0.22 μg/L.45 pesticides were well separated and the linear relationship was good enough which the correlation coefficients were greater than 0.984 for concentrations from 10 to 2000 μg/L for most pesticides. Compared with conventional techniques, it was more accurate, lowcost, highly efficient and environmental friendly. Furthermore, for the limited use of organic solution, it has little harm to environment and human's health for the limited use of organic solution during the analysis process.
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