Simultaneous determination of 11 phenolic compounds in apple vinegar and sugarcane vinegar by HPLC-DAD
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摘要: 利用高效液相色谱联合二极管阵列技术（HPLC-DAD）建立一种可以同时检测不同苹果醋和甘蔗醋中11种酚类物质的分析方法。使用OMNI Hubble C18色谱柱（250 mm×4.6 mm,5μm）,流动相A为甲醇-甲酸（100∶2,v/v）,B为10 mmol/L磷酸氢二钾-磷酸水溶液（200∶1,v/v）,流速为1.0 m L/min,进样量为20μL,梯度洗脱,柱温为35℃,二极管阵列扫描波长范围210400 nm。应用本方法可以检测出不同苹果醋和甘蔗果醋中香草醛、香豆素、绿原酸、咖啡酸、阿魏酸、对香豆酸、芹菜素、木犀草素、肉桂酸、山奈酚、芒柄花黄素等11种酚类物质,其中甘蔗醋中富含绿原酸等10种酚类物质成分。本方法回收率在84.1%118.2%之间,结果准确可靠,简单易行,适合用于各类果醋中酚类物质的分析检测。Abstract: A new method for simultaneous determination of 11 phenolic compounds in fruit vinegar, such as different kinds of apple vinegar and sugarcane vinegar, by high performance liquid chromatography-diode array detector (HPLC-DAD) was established in this study.The phenolic compounds were separated by OMNI BOND with Hubble C18column (250 mm×4.6 mm, 5μm) at 35℃.The mobile phase A was Me OH-HCOOH solution (100∶2, v/v) and the mobile phase B was 10 mmol/L K2HPO4-H3PO4 (200∶1, v/v) , eluted at a flow rate of 1.0 m L/min by a gradient elution program.The injection volume was20μL and the eluted components were monitored at 210400 nm.Under the optimized condition, 11 phenolic compounds in different kinds of apple vinegar and sugarcane vinegar were separated and simultaneous determined, including coumarin, vanillin, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, apigenin, luteolin, cinnamic acid, kaempferol and formononetin, in which 10 phenolic components were found and determined in sugarcane vinegar.The recovery of the above method was 84.1%118.2%.The method is accurate, reliable, simple, feasible and suitable for determination of phenolic compounds in fruit vinegar.
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