Abstract: To investigate the formation and stabilized force of oyster peptide-zinc nanoparticles, the formation conditions could be preliminary screened by turbidity test and the formation of nanoparticles could be confirmed by particle size analysis and electron microscope observation.The stabilized force could be determined through secondary bond destruction experiments. The results showed that nanoparticles whose diameters ranging from 28 ~ 102 nm were formed when 0.5% ~ 0.9% zinc sulfate was added in oyster peptide solvent with p H6.0 ~ 11.0. Zinc was an essential component of nanoparticles, hydrophobic force and electrostatic interaction were the key forces for stabling the nanoparticles. Oyster peptide-zinc nanoparticles were expected to become the new zinc supplements.