澳洲坚果青皮多酚提取工艺优化及其抗氧化活性

张明; 帅希祥; 杜丽清; 涂行浩

doi:10.13386/j.issn1002-0306.2017.22.038

澳洲坚果青皮多酚提取工艺优化及其抗氧化活性

中国热带农业科学院南亚热带作物研究所,农业部热带果树生物学重点实验室

基金项目:

中央级公益性科研院所基本科研业务费专项资金项目(1630062016007)；中国热带农业科学院基本科研业务费专项资金(1630062017018);中国热带农业科学院基本科研业务费专项资金(1630062016012)；公益性行业(农业)科研专项(201303077)；

详细信息

作者简介:
张明 (1989-) , 硕士研究生, 研究方向:休闲农产品加工, E-mail:zhangmingqau@163.com。;

杜丽清 (1976-) , 硕士, 研究员, 研究方向:休闲农业, E-mail:duliqing927618@163.com。;

中图分类号: TS209
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出版历程
- 收稿日期: 2017-05-17

Optimization of extraction and antioxidant activity of polyphenols from macadamia green peel

Key Laboratory of Tropical Fruit Biology of the Ministry of Agriculture, South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences

摘要

摘要: 以新鲜澳洲坚果青皮为原料,研究了澳洲坚果青皮多酚的提取工艺及抗氧化活性。在单因素实验的基础上,通过4因素3水平的正交实验优化澳洲坚果青皮多酚提取工艺,并以Trolox为对照,研究其对DPPH自由基和ABTS+自由基的清除能力以及总抗氧化能力。正交实验结果表明:提取时间为90 min、料液比为1∶50（g/m L）、提取温度为50℃、乙醇体积分数为40%为最佳提取工艺条件,在此条件下澳洲坚果青皮多酚提取量达到（1027.47±10.76）mg/100 g。抗氧化实验结果表明:澳洲坚果青皮多酚对DPPH自由基和ABTS+自由基的半数清除率IC50分别为4.13、112.94 mg/L,且其总抗氧化能力约为Trolox的1.7倍。由此可知,澳洲坚果青皮多酚具有很强的抗氧化能力,可用于制备天然抗氧化剂。
- 澳洲坚果青皮 /
- 多酚 /
- 提取 /
- 抗氧化
Abstract: The study focus on the optimization of extraction and antioxidant activity of polyphenols from fresh macadamia green peel.The four factors and three level orthogonal test was carried out on the basis of single factor experiment. The evaluation of antioxidant activity of the polyphenols that extracted from macadamia green peel was carried out by DPPH, ABTS+radical scavenging and total antioxidant capacity in comparison to Trolox. The optimized conditions were determined as follows:extraction time 90 min, solid-liquid ratio 1∶ 50 ( g/m L) , extraction temperature 50 ℃, 40% ethanol.And the polyphenols yield was up to ( 1027.47 ± 10.76) mg/100 g under the optimized conditions.In the DPPH, ABTS+radical scavenging assays, the IC50 value of the polyphenols that extracted from macadamia green peel were 4.13, 112.94 mg/L, respectively. And the total antioxidant capacity was about 1.7 times of Trolox. Moreover, the antioxidant capacity of the polyphenols that extracted from macadamia green peel was very strong and it can be used to prepare natural antioxidants.
- macadamia green peel /
- polyphenols /
- extraction /
- antioxidant

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