Abstract: Alcohol aqueous solution was used to extract polyphenol from black grain bran with ultrasonic-assisted method.The influences of alcohol concentration, extraction temperature, solid-to-liquid ratio, ultrasonic power, extraction duration and extraction times on yield of polyphenol were investigated.Based on single factor experiment, surface response design was used to optimize the extraction technology.The optimized extraction parameters were as follows: alcohol concentration of 58%, solid-to-liquid ratio of 1 ∶ 22 ( g/m L) , ultrasonic power of 60 W, extraction temperature of 50 ℃, extraction duration and times of37 min and 2 times, respectively. Under the optimized condition, the yield of total polyphenol was the highest, which reached3.545 mg/g. The ptimized technology would be conductive in the industrial extraction of polyphenol from black wheat bran.