超高压提取核桃分心木总黄酮工艺及动力学模型研究

何薇; 严成; 熊雪媛; 唐周刚

doi:10.13386/j.issn1002-0306.2017.21.037

超高压提取核桃分心木总黄酮工艺及动力学模型研究

西南科技大学生命科学与工程学院
盐亭县金土地农业发展有限公司

基金项目:

四川省科技厅重点项目(2016NZYZF0073)；

详细信息

作者简介:
何薇 (1990-) , 女, 硕士研究生, 研究方向:农副产品精深加工, E-mail:vivianweie@163.com。;

严成 (1963-) , 男, 硕士, 教授, 研究方向:食品化学与功能性食品, E-mail:ycx824511@163.com。;

中图分类号: TQ914.1
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出版历程
- 收稿日期: 2017-05-07

Study on technology and kinetic model for ultrahigh pressure extraction of total flavonoids from walnut diaphragm

College of Life Science and Engineering, Southwest University of Science and Technology
Yanting Golden Land of Agriculture and Forestry Development Co., Ltd.

摘要

摘要: 以核桃分心木为原料,利用响应面法对常温下超高压提取核桃分心木中总黄酮的工艺条件进行优化并建立动力学模型。以乙醇体积分数、压力、保压时间、料液比为主要因素,采用Box-Behnken实验设计,研究各因素及交互作用对总黄酮提取得率的影响;同时根据Fick第一扩散定律建立超高压提取分心木总黄酮的动力学方程。结果表明,响应面优化的最佳工艺参数为:乙醇体积分数62%、压力385 MPa、保压时间9 min,料液比1∶25（g/m L）,此条件下,总黄酮的实际提取得率为12.31%,与模型预测值基本相符;动力学模型的预测值与实际值相关性良好,决定系数R2=0.9875。与传统提取方法相比,超高压提取在提高黄酮提取得率的同时能显著缩短提取时间、降低能耗。
- 核桃分心木 /
- 总黄酮 /
- 超高压提取 /
- 响应面分析 /
- 动力学模型
Abstract: Based on walnut diaphragm as the raw material, processing conditions for the ultrahigh pressure extraction of total flavonoids from walnut diaphragm using response surface methodology ( RSM) were optimized. Meanwhile, a kinetics model was developed.The major factors included alcohol concentration, pressure, pressure holding time and solid-liquid ratio, Box-Benhnken experimental design was utilized to investigated the influence of different factors and their interactions on the extraction rate of total flavonoids. Also, kinetic equation of extracting total flavonoids under ultrahigh pressure was developed according to Fick 's first law of diffusion. The results indicated that 62%, 385 MPa, 9 min, and 1 ∶ 25 were the optimum extraction parameters for ethanol concentration, extraction pressure, pressure holding time and solid-liquid ratio, respectively.Under such conditions, the actual extraction rate of total flavonoids was 12.31%, which was consistent with the predicted values.The predicted value of kinetic model was well correlated with the actual value, and the coefficient of determination R2 was 0.9875. Compared with the traditional heating method, the ultrahigh pressure extraction process can significantly increase the extraction rate and shorten the extraction time.
- walnut diaphragm /
- total flavonoids /
- ultrahigh pressure extraction /
- response surface methodology (RSM) /
- kinetic model

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参考文献(20)

[1]	阿巴拜克热·热合曼.维吾尔医常用生药 (维吾尔文) [M].乌鲁木齐:新疆人民卫生出版社, 2004:772.
[2]	南京中医药大学.中药大辞典:上册[M].上海:上海科技出版社, 2006:638.
[3]	李智慧, 孙志宏.山西核桃生产现状及优势分析[J].内蒙古科技与经济, 2014, 19 (22) :23-24.
[4]	杨明珠, 田新雁, 肖朝江, 等.核桃分心木化学成分与生物活性研究[J].天然产物研究与开发, 2012, 35 (12) :1707-1711.
[5]	毕肯·阿不得克里木, 韩艳春, 阿依吐伦·斯马义.核桃分心木化学成分的预试验研究[J].新疆医科大学学报, 2010, 49 (9) :1044-1046.
[6]	赵焕新, 景援朝, 白虹, 等.分心木中的化学成分及抗氧化活性研究[J].中国实验方剂学杂志, 2016, 65 (7) :54-57.
[7]	Middleton E, Kand aswami C, Theoharides TC.The effects of plant flavonoids on mammalian cells:Implications for Inflammation, Heart Disease, and Cancer[J].Pharmacological Reviews, 2000, 52 (4) :673-678.
[8]	张淑兰, 王国军, 唐辉, 等.核桃隔膜中总黄酮的提取工艺优选[J].中国实验方剂学杂志, 2013, 40 (14) :50-52.
[9]	李橙橙, 齐洁, 杨永建.分心木中总黄酮的提取工艺考察[J].中国药师, 2010, 65 (3) :369-371.
[10]	樊振江, 于旺堂, 纵伟.超高压提取柿叶黄酮的研究[J].食品工程, 2008, 33 (2) :46-48.
[11]	Jing S, Wang S, Li Q, et al.Dynamic high pressure microfluidization-assisted extraction and bioactivities of Cyperus esculentus (C.esculentus L.) leaves flavonoids[J].Food Chemistry, 2016, 39 (192) :319-327.
[12]	Shouqin Z, Junjie Z, Changzhen W.Novel high pressure extraction technology[J].International Journal of Pharmaceutics, 2004, 278 (2) :471-474.
[13]	潘兴桥, 李力, 荆旭慧, 等.超高压技术提取蓝莓总黄酮的工艺研究[J].食品工业, 2013, 25 (10) :17-19.
[14]	李宏伟, 张守勤, 窦建鹏, 等.超高压提取山楂叶中黄酮类化合物[J].吉林大学学报 (工学版) , 2006, 78 (3) :438-442.
[15]	Wang Y, Gao Y, Ding H, et al.Subcritical ethanol extraction of flavonoids from Moringa oleifera, leaf and evaluation of antioxidant activity[J].Food Chemistry, 2017, 218 (42) :152-158.
[16]	敬思群, 任志艳, 马泽鑫, 等.响应面法优化超高压提取金鸡菊总黄酮工艺研究[J].食品科技, 2013, 32 (6) :214-219.
[17]	Huang X, Tu Z, Jiang Y, et al.Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of lentinan[J].International Journal of Biological Macromolecules, 2012, 51 (5) :926-32.
[18]	Nicolin D J, Rossoni D F, Jorge L M M.Study of uncertainty in the fitting of diffusivity of Fick’s Second Law of diffusion with the use of bootstrap method[J].Journal of Food Engineering, 2016, 184 (32) :63-68.
[19]	王居伟, 马挺军, 贾昌喜.超高压提取大豆皂苷的工艺优化及动力学模型[J].中国食品学报, 2012, 12 (4) :8-18.
[20]	林亚平, 卢维伦.非溶蚀型药物体系的释放动力学新模型——Fick第一扩散定律的修正及其应用[J].药学学报, 1997, 32 (11) :869-873.

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