Preparation and evaluation of shuanghua plum preserved fruit with qingyan effect
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摘要: 本文以双华李果胚为原料,采用响应面优化四种中草药（金银花、甘草、胖大海、薄荷）提取浓缩液的复配比,结合凉果传统加工工艺研发一种具有清咽润喉功效的双华李凉果,以脂多糖（LPS）诱导的RAW264.7小鼠巨噬细胞为模型,通过对炎症因子（TNF-α、IL-lβ、IL-6、PGE2、NO）分泌的影响评价其清咽润喉效果。结果表明,四种中草药提取浓缩液的最佳复配浓度分别为:金银花56 mg/m L、甘草59 mg/m L、胖大海61 mg/m L、薄荷105 mg/m L。在炎症反应中,一定浓度的样品均能使炎症因子TNF-α、IL-lβ、IL-6、NO的分泌显著降低（p<0.05）,而对PGE2的分泌没有显著性影响（p>0.05）;当浓度达到为50 mg/L时的抑制抑制作用最强,对TNF-α、IL-lβ、IL-6、NO的抑制率分别为54.42%、69.14%、38.50%、27.17%,说明研制的双华李凉果能够抑制炎症因子的分泌,具有清咽润喉功效。Abstract: With shuanghua plum being used as material, the optimum mixture ratio of the extract of four Chinese herbs was optimized by the response surface method using bacteriostatic test in vitro, prepared a functional preserved fruit with qingyan effect was prepared in combination with the traditional processing technology. LPS-induced RAW264.7 macrophage inflammatory model was adopted to determine the anti-inflammatory activity of shuanghua plum preserved fruit using inflammatory cytokines ( TNF-α, IL-lβ, IL-6, PGE2, NO) as indicators.The results showed that the optimal combination ratio of the extract of the four herbs were honeysuckle 56 mg/m L, licorice 59 mg/m L, sterculia scaphigera 61 mg/m L and peppermint105 mg/m L. In the inflammatory response, the samples at appropriate concentrations could decrease inflammatory cytokines ( TNF-α, IL-lβ, IL-6, NO) significantly ( p < 0.05) , while there was no significant effect on PGE2 secretion ( p > 0.05) . The strongest inhibitory effect of the extract of shuanghua plum preserved fruit was obtained at the concentration of 50 mg/L.Under this concentration, the inhibition rate against TNF-α, IL-l, IL-6 and NO was 54.42%, 69.14%, 38.50% and 27.17%, respectively, demonstrating the anti-inflammatory effect of the developed shuanghua plum preserved fruit by decomposing the inflammatory factors.This showed that the shuanghua plum preserved fruit can inhibit the secretion of inflammatory factors, with qingyan effect.
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