Study on the preparation of isomalto-oligosaccharide by enzymatic methods of acorn starch
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摘要: 对橡子粉酶法制备低聚异麦芽糖的工艺过程进行优化,以期提高低聚异麦芽糖中异麦芽糖、潘糖和异麦芽三糖的含量。采用Box-Benhnken响应面法,优化以耐高温α-淀粉酶液化橡子粉的工艺条件。最佳的液化工艺条件为:以DE值13%为最佳液化指标,液化时间31 min、液化温度95℃、液化p H6.6、酶添加量13 U/g,结合生产实际最佳条件下的DE值为12.91%;继而采用正交实验,优化以普鲁兰酶、β-淀粉酶糖化橡子粉液化液的工艺条件,得到最佳的糖化工艺条件为:普鲁兰酶添加量25 U/g、β-淀粉酶添加量130 U/g、温度60℃、时间10 h,在此最佳工艺条件下糖化转苷后的（IG2+P+IG3）含量为36.11%±0.17%;转苷工艺过程的α-葡萄糖转苷酶最佳添加量为1.5 U/g,最佳条件下异麦芽糖、潘糖、异麦芽三糖含量之和为36.27%±0.18%。Abstract: With the acorn starch as raw material, the process of preparation of isomalto-oligosaccharide by enzymatic methods were optimized in order to increase the content of isomaltose, panose and isomaltotriose of isomalto-oligosaccharide. Box-Benhnken response surface analysis method was used to optimize the process conditions of liquefying acorn starch with thermostable α-amylase. The results showed that the DE value of 13% as the best liquefaction index, the optimal liquefaction conditions were 31 min of liquefaction time, 95 ℃ of liquefaction temperature, 6.6 of liquefying p H value, 13 U/g of thermostableα-amylase, at this point, in combination with the production practice, the value of the liquefied liquid DE was 12.91%, then through single factor experiments, the process conditions of saccharifying acorn starch liquefying liquor with pullulanase and glucoamylase were investigated, the saccharification conditions were optimized to be 25 U/g of pullulanase, 130 U/g of glucoamylase, 60 ℃ of saccharification temperature and 10 h of saccharification time, at this optimal condition, the content of ( IG2+ P + IG3) was predicted to 36.11% ± 0.17%, the optimum concentration of α-glucosidase in transglycosylation process was1.5 U/g, under the optimal condition, the total content of isomaltose, panose, and isomaltotriose were 36.27% ± 0.18%.
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Keywords:
- isomalto-oligosaccharide /
- acorn starch /
- liquefaction /
- saccharification /
- transglycosylation /
- technology
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