Optimization of preparation of Tibetan sheep placenta peptide by ultrasonic-assisted composite enzymatic hydrolysis
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摘要: 为实现羊胎盘深度开发利用,以肽得率和水解度为指标,筛选确定多种蛋白酶复合水解的最佳组合方案,并利用响应面法优化超声波辅助复合酶法水解制备羊胎盘肽的预处理参数。结果表明,响应面法优化后的超声预处理参数为超声时间18 min、温度28℃和功率420 W,超声波预处理后羊胎盘经木瓜蛋白酶和风味酶联合同步复合酶解制备羊胎盘肽的肽得率为39.89%,水解度为21.78%;该最优条件下平均肽链长度为4.59,分子量约500~600。结论:超声波辅助复合酶法制备藏系绵羊胎盘肽的工艺可行且有效。Abstract: In order to realize the development and utilization of sheep placenta, the optimal combination scheme for the composite enzymatic hydrolysis of multiple proteases was investigated with the indicators of the yield of peptide ( PY) and the degree of hydrolysis ( DH) .On this basis, the ultrasonic pretreatment parameters of sheep placenta were optimized by response surface methodology ( RSM) .The results showed that the optimal conditions of ultrasound pretreatment were found as follows:ultrasonic time of 18 min, temperature of 28 ℃ and power of 420 W. Under these optimal ultrasound pretreatment conditions, synchronous composite enzymatic preparation by papain and flavor enzyme of the PY was 39.89%, the DH was 21.78%.And the average chain length was 4.59 and the molecular weight was about 500 ~ 600. Conclusion: the ultrasonic assisted enzymatic preparation method was feasible and effective in the preparation of Tibetan sheep placenta peptide.
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Keywords:
- Tibetan sheep /
- sheep placenta peptide /
- papain /
- flavor enzyme /
- ultrasonic pretreatment /
- process optimization
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