Comprasion of the hypolipidemic activities of water extractions and ethanol extractions from red resberry
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摘要: 以高脂饲料建立高血脂症大鼠模型,将红树莓水提取物、50%乙醇提取物、100%乙醇提取物按照大鼠体重以50 mg提取物/（kg·d）对高血脂症大鼠进行灌胃10周,并以等量生理盐水灌胃普通对照组和高脂对照组。通过测定灌胃前后大鼠体重、计算脏器指数和动脉粥样硬化指数（AI）的变化和大鼠血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）水平、肝脏中TC和TG水平、粪便中TC和胆汁酸（TBA）含量的变化,研究红树莓提取物的降血脂功能。结果表明,与普通和高脂对照组相比,本实验建立的高血脂模型中大鼠的心脏、肾脏、胰腺和肝脏指数并无显著变化,且红树莓提取物能极显著降低高血脂大鼠的AI值（p<0.01）。同时红树莓水提取物和乙醇提取物均能降低血清中TC、TG及LDL-C水平和肝脏中TC、TG水平,升高高血脂症大鼠血清HDL-C水平,促进高血脂症大鼠体内TC和TBA的排出,并抑制高血脂大鼠体重增加,且其乙醇提取物的效果比水提物更加显著。研究表明红树莓提取物具有降血脂功能,且乙醇提取物的降血脂效果优于水提取物。Abstract: The hyperlipidemic rat models induced by high-fat feeding were given a gavage of the water extractions, 50%ethanol extractions and 100% ethanol extractions from red raspberry, respectively, for 10 weeks.The gavage amounts depended on 50 mg of extractions per gram of mice weight ( kg·d) and the control group and hyperlipidemic group was treated with normal saline.Some biochemical parameters were detected before and after gavage to evaluate the hypolipidemic activity of red raspberry extractions, including the changes of the body weights and the viscera indexes and atherosclerosis index ( AI) , the levels of serum total cholesterol ( TC) , triglyceride ( TG) , high-density lipoprotein cholesterol ( HDL-C) and low density lipoprotein cholesterol ( LDL-C) , the levels of TC and TG in liver, the levels of TC and total bile acids ( TBA) in faeces of rat model.The results showed that the viscera indexes ( such as heart, kidney, pancreas and liver indexes) of such induced rat models had not changed significantly before and after test, but the AI was significantly reduced ( p < 0.01) after being given the gavage of the red raspberry extractions. Both water extractions and ethanol extractions from red raspberry could decrease the levels of serum TC, TG, LDL-C and the levels of TC, TG in liver and could increase the serum HDL-C levels in such rat models when compared with the control group and hyperlipidemic group.In addition, the red raspberry extractions were proved to have the ability of improving the excretion of TC and TBA and inhibiting the increase of body weights of the hyperlipidemic rat models.And the ethanol extracts from red raspberry showed better effects on the experimentally induced rat models than the water extractions.In conclusion, the red raspberry extractions had the hypolipidemic activity and the ethanol extractions showed better effects on hyperlipidemic rat models.
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Keywords:
- rat models /
- red raspberry /
- extractions /
- the hypolipidemic activity
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