毛细管区带电泳非衍生化直接测定蜂蜜中糖含量

王守箐

doi:10.13386/j.issn1002-0306.2017.20.048

毛细管区带电泳非衍生化直接测定蜂蜜中糖含量

王守箐

临沂大学药学院

详细信息

作者简介:
王守箐 (1961-) , 男, 硕士, 副教授, 研究方向:药物分析, E-mail:wangshouqing@lyu.edu.cn。;

中图分类号: O658.9;S896.1
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出版历程
- 收稿日期: 2017-03-31

Direct determination the content of carbohydrates in honey by underivatized capillary zone electrophoresis

WANG Shou-qing

School of Pharmacy, Linyi University

摘要

摘要: 建立了一种新的测定蜂蜜中糖含量的方法,并探讨了检测机理。以弹性石英毛细管柱（有效长度/总长度=52/62 cm）为分离柱,130 mmol/L的Na OH+30 mmol/L的Na2HPO4溶液（p H=12.8）为运行缓冲液,温度20℃,分离电压在1 min内升至16 k V,测定波长为270 nm,以20 psi的压力进样,内标峰面积法计算含量。经测定:蜂蜜样品中果糖、葡萄糖、蔗糖和麦芽糖含量分别为42.8%、36.1%、4.67%和2.16%,检测机理可能是糖在强碱溶液中发生光化学反应,检测在非平衡态进行;果糖、葡萄糖在0.04⁰.8 mg/m L,蔗糖、麦芽糖在0.005⁰.1 mg/m L范围内线性关系良好;最低检测限果糖和葡萄糖为9μg/m L,蔗糖和麦芽糖为18μg/m L,明显低于高效液相色谱示差检测法;果糖、葡萄糖、蔗糖、麦芽糖回收率分别为99.4%、97.9%、97.1%、102.7%,方法回收率良好。此方法准确、简便,可用于蜂蜜中糖的测定。
- 非衍生化 /
- 毛细管区带电泳 /
- 蜂蜜 /
- 糖
Abstract: A new method was established to determine the content of carbohydrates in honey, and the detection mechanism was expounded. An elastic quartz capillary tube ( effective length/total length = 52/62 cm) was used with a running buffer of130 mmol/L Na OH + 30 mmol/L Na2HPO4 ( p H = 12.8) at 20 ℃, the separation voltage was raised linearly within 1 min from0 k V to + 16 k V, the detection wavelength was 270 nm. The pressure of injection was 20 psi, the contents were calculated by internal standard method.The contents of fructose, glucose, sucrose and maltose in honey samples were 42.8%, 36.1%, 4.67%and 2.16%, respectively.The detection mechanism may be photochemical reaction of carbohydrates in the alkali solution and detection was carried out in non-equilibrium state.The linear ranges of fructose and glucose were 0.04⁰.8 mg/m L, sucrose and maltose were 0.005⁰.1 mg/m L.The detection limits ( at S/N of 3) of fructose, glucose, sucrose and maltose were 9, 9, 18 and18 μg/m L which were significantly lower than that of high performance liquid chromatography-refractive index detector ( HPLC-RID) .The recoveries of fructose, glucose, sucrose and maltose were 99.4%, 97.9%, 97.1% and 102.7%, respectively. This method is accurate and simple which can be used for determining the contents of carbohydrates in honey.
- underivatization /
- capillary zone electrophoresis (CZE) /
- honey /
- carbohydrates

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