Optimization of preparation process of evening primrose oil-β-cyclodextrin inclusion complex by central composite design response surface method
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摘要: 为了优化月见草油-β-环糊精包合物的制备工艺。采用饱和水溶液法制备月见草油β-环糊精包合物,以β-环糊精与月见草油的投料比、包合温度和包合时间为考察因素,月见草油包合物包合率和包合物得率的综合评分为指标,通过星点设计-响应面法优化制备工艺,经红外分析和差示扫描量热进行包合物形成的验证。最佳包合工艺为β-环糊精与月见草油投料比为5∶1 m L/g、包合温度55℃、包合时间1.8 h,在此最佳工艺条件下,月见草油-β-环糊精包合物的包合率和包合物得率分别为81.56%和92.28%。实验证明月见草油可与β-环糊精形成稳定的包合物,为月见草油的应用开发提供了理论基础。Abstract: The aim of this research is to optimize the preparation process of evening primrose oil-β-cyclodextrin inclusion complex.β-cyclodextrin inclusion of volatile oil from evening primrose oil was prepared by saturated aqueous solution method.Central composite design-response surface method was employed to optimize the preparation process, with the feed ratios, inclusion temperature and inclusion time as factors.Moreover, the comprehensive scores of inclusion rate and yield of inclusion complex were selected as the target. The inclusion complex of evening primrose oil was validated by infrared and differential scanning calorimetry method.The optimum preparation process was as follows: feed ratio 5∶ 1 ( β-cyclodextrin: evening primrose oil, g/m L) , the temperature was 55 ℃, and the time of inclusion was 1.8 h. Under the optimum conditions, the inclusion rate and the yield of the inclusion compound were 81.56% and 92.28% respectively.The result demonstrated that evening primrose oil and β-CD could form stable inclusion complexes.Additionally, the result of this experiment provided a theoretical basis for the application and development of the evening primrose oil.
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Keywords:
- evening primrose oil /
- β-cyclodextrin /
- inclusion complex /
- response surface method
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