Effect of high pressure combined with thermal treatment on the taste of pork
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摘要: 为研究高压结合热处理对猪肉滋味物质的影响,以猪背最长肌为原料,用不同压力(200~600 MPa)结合热(20~60℃)处理10 min,然后测定各样品氨基酸组成、核苷酸组成、还原糖含量、硫胺素含量、脂肪酸组成,并用电子舌对样品的整体滋味进行评价。高压结合热处理极显著(p<0.01)影响猪肉中游离氨基酸总含量和组成;核苷酸及降解产物总含量极显著(p<0.01)增加,特别是次黄嘌呤核苷酸(IMP);还原糖和硫胺素含量都显著降低(p<0.05);但高压结合热处理对猪肉总脂肪酸组成影响不明显(p>0.05)。偏最小二乘回归(APLSR)分析表明压力和温度对各样品滋味的影响程度相当;电子舌分析表明处理条件越剧烈样品的整体滋味和对照样的差异越大,在200和400 MPa下温度对整体滋味的影响较大,而在600 MPa下温度的作用很小。在实际应用中为了更好地保留新鲜肉的滋味应避免采用600 MPa或60℃的条件对猪肉进行处理。Abstract: To investigate the effect of high-pressure combined with thermal treatment on the taste compounds of pork, the longsissimus muscles were treated under a pressure range of 200600 MPa and a temperature range of 20 60 ℃ for 10 min.Then the composition of amino acids, nucleotides and fatty acids, and the content of reducing sugar and thiamine in samples were analyzed, and the whole taste of samples was compared by electronic tongue. The results showed that high-pressure combined with thermal treatment had a significant effect on the content and composition of free amino acids. The content of nucleotide and its degradation products increased significantly after treatment, especially for hypoxanthine nucleotide ( IMP) , while that of reducing sugar and thiamine decreased significantly ( p < 0.05) . High-pressure combined with thermal treatment had not a significant effect ( p > 0.05) on the fatty acid composition of total lipids. Analysis of partial least squares regression ( APLSR) showed that the effect of pressure and temperature on the taste of samples was comparative.The results of electronic tongue showed that the more severe the treatment conditions were, the more of samples' whole taste changed, when treated with200 or 400 MPa, temperature had a marked effect on the taste of samples, while when treated on 600 MPa, the role of temperature was very limited.In practice, the treatment at 600 MPa or 60 ℃ should be avoided in order to better preserve the taste of fresh meat.
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Keywords:
- pork /
- high pressure /
- thermal treatment /
- taste compounds
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[1] 段虎, 王祎娟, 马汉军.超高压处理对肉及肉制品食用品质的影响[J].食品与机械, 2011, 27 (1) :151-154. [2] Torres J A, Velazquez G.Commercial opportunities and research challenges in the high pressure processing of foods[J].Journal of Food Engineering, 2005, 67 (1-2) :95-112.
[3] 赵俊芳, 赵玉生, 姚二民.超高压技术处理食品的特点[J].食品科技, 2006 (10) :11-13. [4] 吕东坡, 朱仁俊.猪肉中风味物的研究进展[J].食品工业科技, 2009, 30 (8) :352-355. [5] 成坚, 刘晓艳.加热过程对鸡肉风味前提物质的影响[J].食品与发酵工业, 2005, 31 (1) :146-148. [6] 刘源, 徐幸莲, 王锡昌, 等.不同加工对鸭肉滋味成分的作用研究[J].食品科学, 2008, 29 (3) :127-130. [7] 马汉军.高压和热结合处理对僵直后牛肉品质的影响[D].南京:南京农业大学, 2004. [8] Mcardle R, Marcos B, Kerry J P, et al.Monitoring the effects of high pressure processing and temperature on selected beef quality attributes[J].Meat Science, 2010, 86 (3) :629-634.
[9] Mcardle R A, Marcos B, Kerry J P, et al.Influence of HPP conditions on selected beef quality attributes and their stability during chilled storage[J].Meat Science, 2011, 87 (3) :274-281.
[10] Mcardle R A, Marcos B, Mullen A M, et al.Influence of HPP conditions on selected lamb quality attributes and their stability during chilled storage[J].Innovative Food Science and Emerging Technologies, 2013, 19 (7) :66-72.
[11] Huang Y, Gan Y, Li F, et al.Effects of high pressure in combination with thermal treatment on lipid hydrolysis and oxidation in pork[J].LWT-Food Science and Technology, 2015, 63 (1) :136-143.
[12] Huang Y, Wang Y, Wu Z, et al.Combined effects of highpressure and thermal treatments on lipid oxidation and enzymes in pork[J].Food Science and Biotechnology, 2016, 25 (1) :261-266.
[13] Huang Y, Wu Z, Wang Y, et al.Examination of the effects of temperature and pressure on lipoxygenase activities in pork using response surface methodology[J].Food Science and Biotechnology, 2015, 24 (4) :1257-1263.
[14] 黄甜, 严成, 孙娟, 等.超高压结合热处理对猪肉肌内脂肪酸组成的影响[J].现代食品科技, 2015, 31 (5) :226-236. [15] 王俊, 胡桂仙, 于勇, 等.电子鼻与电子舌在食品检测中的应用研究进展[J].农业工程学报, 2004, 20 (2) :292-295. [16] 秦刚.荣昌猪肉在不同烤制温度中挥发性风味物质的研究[D].重庆:西南大学, 2011. [17] GB/T 9695.27-2008肉与肉制品维生素B1含量的测定[S].北京:中国标准出版社, 2008. [18] GB/T 5009.7-2008食品中还原糖的测定[S].北京:中国标准出版社, 2008. [19] Folch J, Lees M, Sloane-Stanley G.A simple method for the isolation and purification of total lipids fromanimal tissues[J].Journal of Biological Chemistry, 1957, 226 (1) :497-509.
[20] Morrison W, Smith L.Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol[J].Journal of Lipid Research, 1964, 5 (4) :600-608.
[21] Suzuki A, Homma N, Fukuda A, et al.Effects of high pressure treatment on the flavor-related components in meat[J].Meat Science, 1994, 37 (3) :369-379.
[22] Campus M, Flores M, Martinez A, et al.Effect of high pressure treatment on colour, microbial and chemical characteristics of dry cured loin[J].Meat Science, 2008, 80 (4) :1174-1181.
[23] 马海建, 施文正, 宋洁, 等.超高压处理对草鱼鱼肉风味物质的影响[J].现代食品科技, 2016, 32 (8) :204-212. [24] 严子钧, 施文正, 齐自元, 等.超高压对鳙鱼鱼糜风味的影响[J].食品工业科技, 2014, 35 (24) :107-112. [25] 常耀光, 李兆杰, 薛长湖, 等.超高压处理对南美白对虾在冷藏过程中贮藏特性的影响[J].农业工程学报, 2008, 24 (12) :230-237. [26] Lopez-caballero M, Goncalves A, Nunes M L.Effect of CO2/O2-containing modified atmospheres on packed deepwater pink shrimp (Parapenaeus longirostris) [J].European Food Research and Technology, 2002, 214 (3) :192-197.
[27] 岳志国.超高压条件下绵羊肌肉风味物质变化的研究[D].呼和浩特:内蒙古农业大学, 2002. [28] 姜雪, 于鹏, 肖杨, 等.超高压处理对生鲜牛乳品质的影响[J].食品科技, 2015, 40 (4) :99-102. [29] Hugas M, Garriga M, Monfort J M.New mild technologies in meat processing:high pressure as a model technology[J].Meat Science, 2002, 62 (3) :359-371.
[30] 方亮.超高压处理对猕猴桃果汁杀菌钝酶效果和品质的影响[D].无锡:江南大学, 2008. [31] 马汉军, 赵良, 潘润淑, 等.高压对肉类基本成分的影响[J].食品与机械, 2006, 22 (3) :150-152.
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