Determination of proanthocyanidins from mangosteen husk by thiolysis-HPLC and normal-phase HPLC
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摘要: 为分析山竹壳中原花青素的组成和含量,采用硫解衍生法和反相HPLC对山竹壳中可提原花青素（EP）和不可提原花青素（NEP）进行了测定,采用正相HPLC、ESI-MS对不同聚合度EP进行了分析和定量。山竹壳中原花青素的主要组成单体为表儿茶素,EP和NEP含量分别为（131.69±11.24）、（36.92±2.38）g/kg（以原花青素B2计）,平均聚合度为（4.27±0.08）、（5.41±0.04）。检测出三种不同聚合度EP分别为:B型原矢车菊素二聚体（40.51±8.30）g/kg、三聚体（38.70±8.69）g/kg和四聚体（13.95±3.93）g/kg。这些结果为山竹壳原花青素的利用提供了一定的依据。Abstract: To investigate the content of proanthocyanidins from mangosteen husk, thiolysis-HPLC was used to analyze and quantify extractable ( EP) and nonextractable ( NEP) proanthocyanidins, then normal-phase HPLC was applied to isolate and determinate EP in different degrees of polymerization.Proanthocyanidins from mangosteen husk mainly consisted of epicatechin, contents and mean degree of polymerization of EP and NEP were ( 131.69 ± 11.24) , ( 36.92 ± 2.38) g/kg procyanidin B2/kg and ( 4.27 ± 0.08) , ( 5.41 ± 0.04) respectively.Contents of B-type procyanidin dimer, trimer and tetramer from EP were ( 40.51± 8.30) , ( 38.70 ± 8.69) , ( 13.95 ± 3.93) g/kg. These results can provide reference for the use of proanthocyanidins from mangosteen husk.
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Keywords:
- Garcinia mangostana L; /
- husk; /
- proanthocyanidins; /
- thiolysis; /
- determination;
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