Analysis of chemical constituents of Ganoderma lucidum and its antioxidant activity in polysaccharides and triterpenoids
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摘要: 本研究测定了树舌灵芝子实体的粗多糖、粗蛋白、粗脂肪、矿物质元素以及三萜的含量,分析了其氨基酸、脂肪酸及矿物质元素组成,并通过测定DPPH·与HO·的清除率,研究三萜及多糖的抗氧化活性。研究结果表明,树舌灵芝子实体含有总糖43.8%,三萜1.75%,蛋白质11.4%,粗脂肪0.40%,灰分1.80%,维生素C 10.5%,维生素A 15.6%;还包含丰富的矿物质元素如Ca、Fe、Zn等,其含量分别为5740、29.7、15.6 mg/kg。树舌灵芝子实体还富含甲硫氨酸(19.3 g/kg)、天冬氨酸(5.5 g/kg)、缬氨酸(19.4 g/kg)、亮氨酸(4.2 g/kg)以及亚油酸(340.9 mg/kg)等,是一种营养丰富且气味独特的食药用菌,通过测定DPPH·的清除率与HO·的清除率得知,三萜与多糖均具有一定的抗氧化活性,且三萜的抗氧化能力比多糖强。Abstract: In this study, crude protein, amino acid, crude fat, mineral and triterpenes contents of the Ganoderma applanatum fruiting bodies were measured.Simultaneously, the compositions of amino acid, fatty acid and mineral were analyzed.Afterwards, the antioxidant activity of total polysaccharides and triterpenoids from Ganoderma applanatum were investigated through determining the clearance rate of DPPH· and HO·.The results showed that the total sugar content of Ganoderma applanatum was 43.8%, and the contents of triterpene, protein, crude fat, ash, vitamin C and vitamin A were 1.75%, 11.4%, 0.40%, 1.80%, 10.5% and 15.6% respectively.It also contained abundant mineral elements such as Ca, Fe, Zn and so on, the contents were 5740, 29.7, 15.6 mg/kg respectively. Moreover, the fruiting bodies of Ganoderma applanatum, which contained methionine ( 19.3 g/kg) , aspartic acid ( 5.5 g/kg) , valine ( 19.4 g/kg) , leucine ( 4.2 g/kg) , linoleic acid ( 340.9 mg/kg) and others, are a nutrient-rich and unique flavor of medicinal bacteria. By measuring the clearance rate of DPPH· and HO·, it was found that triterpenes and polysaccharides had some antioxidant activity, and the antioxidant capacity of triterpenoids was stronger than that of polysaccharides.
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