Effects of activities and gene expression of pathogenesis-related proteins in muskmelon fruit inoculated with Alternaria alternata
-
摘要: 以抗病能力强的伽师瓜和抗病能力较弱的86-1甜瓜作为实验材料,研究甜瓜果实应答链格孢侵染过程中对几丁质酶（CHT）、β-1,3-葡聚糖酶（GLU）活力变化的影响及基因表达规律。伽师瓜和86-1甜瓜接种浓度为1×106个/m L A.alternata孢子悬浮液20μL,置于68℃冷库贮藏,测定病斑大小,几丁质酶和β-1,3-葡聚糖酶活力及基因相对表达量。结果表明:损伤接种A.alternata后,伽师瓜和86-1甜瓜均在15 d发病,贮藏过程中伽师瓜果实的发病率及病斑大小均低于86-1甜瓜;甜瓜应答侵染过程中,几丁质酶和β-1,3-葡聚糖酶活力呈现先升高后下降的趋势,分别于21 d,18 d达到酶活高峰,且接种伽师瓜酶活力显著高于86-1甜瓜（p<0.05）,伽师瓜通过增强病程相关酶活力来抵御链格孢的侵染;伽师瓜CHT1、CHT2、GLU基因表达量显著高于86-1甜瓜,分别在贮藏18、18、15 d表达量最大;贮藏021 d病程相关蛋白活力及基因相对表达量与病斑直径呈正相关,说明病程相关蛋白在植物抵御病原微生物侵染早期与中期中起到了关键作用,并且在侵染中期作用显著。Abstract: Jiashi muskmelon and 86-1 muskmelon species with the characteristics of disease resistance were used as experiment material.This study was aimed to the activities of CHT, GLU and the gene expression rules for CHT and GLU in muskmelon fruits inoculated with A.alternata were analyzed.Jiashi muskmelon and 86-1 muskmelon were inoculated with 20 μL A.alternata spore suspension, inoculation with 20 μL of sterile water as a blank control, the materials were transferred to cold storage at68 ℃, determination of lesion size, the activities of CHT, GLU and the gene expression of CHT and GLU.The results showed that the incidence and the lesion size of Jiashi muskmelon were lower than that of 86-1 muskmelon during the inoculated with A.alternata after 15 days.During the infection period, the activities of CHT, GLU was significantly increased with a trend from rise to decline. The peak of activities for CHT, GLU revealed of 21 d, 18 d. The activities of CHT, GLU in Jiashi melon significantly higher than 86-1 muskmelon Jiashi muskmelon to resist the infection of A.alternaria by enhancing the activity of protease and maintaining the enzyme activity at a higher level. The relative expressions for CHT1, CHT2, GLU were higher in Jiashi muskmelon than 86-1 muskmelon during the whole infection period.The expression levels of CHT1, CHT2 and GLU were significantly higher than 86-1 melon, and the expression level of CHT1, CHT2 and GLU was the highest in storage at 18, 18 and15 days.The relative expression of protein-related protein and gene expression were positively correlated with lesion diameter for 021 d.These results suggest that the pathogenesis related proteins play a key role in the early and middle stage of plant pathogen resistance.
-
Keywords:
- muskmelon /
- Alternaria alternata /
- pathogenesis-related proteins /
- gene expression
-
[1] 薛耀碧, 周雅涵, 邓丽莉, 等.采后果实病程相关蛋白研究进展[J].食品工业科技, 2015, 36 (6) :391-393, 399. [2] Durrant W E, Dong X.Systemic aquired resistance[J].Annual Review of Phytopathology, 2004, 42 (1) :185-209.
[3] 范旭东.柑橘衰退病毒侵染对寄主植物同工酶影响和诱导表达病程相关蛋白的分析[D].武汉:华中农业大学, 2008. [4] 刘纪元, 佟少明, 侯和胜.植物病程相关蛋白研究进展[J].黑龙江科技信息, 2012 (5) :65. [5] 葛永红, 李灿婴, 朱丹实, 等.采后BTH处理对苹果果实苯丙烷代谢和病程相关蛋白积累的增强作用[J].食品工业科技, 2015 (5) :306-310. [6] 郭红莲, 路玉蓉, 王刚志, 等.水杨酸对采后番茄抗逆性及病程相关蛋白的影响[J].现代食品科技, 2012 (2) :131-134. [7] 刘新华.BTH防治芒果采后炭疽病及其系统获得抗性机理[D].海口:海南大学, 2010. [8] 范三红, 李静, 施俊凤.拮抗菌Burkholderia contaminans对玫瑰香葡萄采后灰霉病的抗性诱导[J].食品科学, 2016 (2) :266-270. [9] 张培岭, 白羽嘉, 黄伟, 等.不同浓度外源水杨酸对甜瓜果实抗病相关酶活性的影响[J].食品科技, 2016 (7) :38-43. [10] 白羽嘉, 张培岭, 黄伟, 等.链格孢侵染采后甜瓜果实组织几丁质酶和β-1, 3-葡聚糖酶基因表达分析[J].食品科学 (待刊) . [11] 曹建康, 姜微波, 赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社, 2007. [12] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and 2-ΔΔCt method[J].Methods, 2001, 25 (4) :402-408.
[13] 赵利辉, 邱德文, 刘峥.植物SAR和ISR中的乙烯信号转导网络[J].生物技术通报, 2006 (3) :28-32. [14] 王廷璞, 马静静, 赵菲佚.β-l, 3-葡聚糖酶和几丁质酸在农作物病虫害防治中的研究进展[J].安徽农业科学, 2010, 38 (26) :14417-14419. [15] 罗晶晶, 张仁英, 齐晓花, 等.黄瓜几丁质酶基因克隆及与白粉病抗性关系的初步研究[J].分子植物育种, 2015, 13 (7) :1584-1591. [16] 侯晓婉, 徐碧玉, 胡伟, 等.几丁质酶及基因参与水杨酸诱导香蕉枯萎病抗性[J].广东农业科学, 2015 (7) :64-70, 2. [17] 高丽娟.水杨酸诱导梨抗轮纹病作用机制研究[D].保定:河北农业大学, 2013. [18] 孙溶溶, 彭真, 程琳, 等.BTH诱导花椰菜对菌核病的抗性研究[J].植物病理学报, 2012 (3) :281-289. [19] 吴芳芳, 郑有飞, 万长健, 等.UV-B辐射增强对苹果采后炭疽病发病情况和抗病相关酶活性的影响[J].生态环境, 2008 (3) :962-965.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
