一株产几丁质脱乙酰酶海洋细菌的筛选、鉴定及发酵优化

顾张慧; 刘姝; 胡晟源; 来蒋丽; 王淑军; 焦豫良; 房耀维

doi:10.13386/j.issn1002-0306.2017.18.025

一株产几丁质脱乙酰酶海洋细菌的筛选、鉴定及发酵优化

1. 淮海工学院海洋生命与水产学院
2. 江苏省海洋资源开发研究院

基金项目:

国家自然科学基金(31201687)；中国博士后科学基金资助项目(2016M592245)；教育部留学回国人员科研启动基金；江苏省自然科学基金面上项目(BK20151282)；江苏省六大人才高峰项目(SWYY-195)；江苏省高校“青蓝工程”；山东省博士后创新项目；淮海工学院科研创新基金(Z2014016)；中国博士后科学基金(160034)；青岛博士后基金；

详细信息

作者简介:
顾张慧 (1994-) , 女, 硕士研究生, 研究方向:海洋微生物生物技术, E-mail:18262792387@163.com。;

房耀维 (1978-) , 男, 博士, 副教授, 研究方向:海洋微生物生物技术, E-mail:foroei@163.com。;

中图分类号: TS201.3
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出版历程
- 收稿日期: 2017-03-19

Screening and identification of a marine bacteria producing chitin deacetylase and optimization of its fermentation conditions

College of Marine Science and Technology, Huaihai Institute of Technology
Jiangsu Marine Resources Development Research Institute

摘要

摘要: 目的:从海洋样品中筛选产几丁质脱乙酰酶细菌,对菌株进行鉴定和发酵条件优化。方法:利用显色平板从黄海海州湾燕尾港海域的海泥中筛选几丁质脱乙酰酶产生细菌。利用形态学、生理生化特性以及16S r DNA序列分析对菌株进行鉴定。利用单因素实验和正交实验优化菌株发酵条件。结果:筛选获得几丁质脱乙酰酶产生菌MCDA01,经形态学特征、生理生化特性以及16S r DNA序列分析将菌株鉴定为天目不动杆菌(Acinetobacter schindleri)。菌株最佳生长条件为30℃,p H8.0,Na Cl 4%。最佳产酶发酵条件为:发酵时间60 h,发酵温度20℃,培养基起始p H7,装液量20%,诱导剂几丁质浓度1%。在此条件下,产酶量达到201.37 U/m L,比优化前提高了4.14倍。结论:几丁质脱乙酰酶产生菌MCDA01产酶量达到201.37 U/m L,有工业应用潜力。
- 几丁质脱乙酰酶 /
- 菌株筛选 /
- 鉴定 /
- 发酵优化
Abstract: Objective: Screen chitin-deacetylase-producing strains from marine samples, identify the strain and optimize its fermentation conditions.Methods: Chitin-deacetylase-producing strains were screened by color culture medium from the marine mud of Yanwei Harbor. The strains were identified by morphological, physiological and biochemical characteristics and16 S r DNA sequence analysis. The single factor and orthogonal experimental design were used to optimize the fermentation conditions.Results: Strain MCDA01 producing chitin deacetylase was screened, and it was identified as Acinetobacter schindleri through morphological, biochemical characteristics, and 16 S r DNA sequence analysis. The optimal culture conditions of strain MCDA01 was obtained as follows: 30 ℃, p H8.0, and Na Cl 4%.The optimal fermentation conditions was obtained as follows: the fermentation time was 60 h, the fermentation temperature was 20 ℃, the culture medium starts p H was 7, the liquid loading amount was 20%, and the inducer chitin concentration was 1%. Under this condition, production of enzyme was up to201.37 U/m L, which was increased by 4.14 times. Conclusion: The maximum of enzyme production were optimized to201.37 U/m L, and have industrial application potential.
- chitin deacetylase /
- strain screening /
- identifiction /
- fermentation optimization

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