Research on the compound enzymatic extraction process of pectin from passionflower peel
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摘要: 实验以西番莲果皮为原料,以酶解pH、酶解时间、酶解温度、酶浓度与液料比为单因素,分别研究了四种酶(纤维素酶、半纤维素酶、木质素酶、淀粉酶)对西番莲果皮中果胶提取的影响,并确定将纤维素酶、半纤维素酶与木质素酶三者进行复配,然后以酶解p H、酶解时间、酶解温度和液料比为因子进行四因素三水平正交实验,以优化复合酶酶解工艺,最后通过响应面实验,确定了复合酶的添加量。实验优选得复合酶最适配比和最适酶解提取条件为:将纤维素酶0.8 g/100 g、半纤维素酶1.2 g/100 g、木质素酶0.2 g/100 g进行复配,液料比为6∶1 m L/g,p H为4,提取温度40℃,提取3.5 h,此时西番莲果皮的果胶提取得率可以达到2.63%±0.021%。Abstract: Passionflower peel was used as raw materials in the reseach.With enzyme solution p H, hydrolysis time, hydrolysis temperature, enzyme concentration and ratio of liquid to material as single factors, the effects of four kinds of enzyme ( cellulase, hemicellulase and lignin enzymes, amylase) on pectin extraction from the passionflower peel were studied. And the enzyme combination consists cellulose, hemicellulase and lignin enzymes were determined. Through L9 ( 34) orthogonal experiment and response surface test, the enzymatic hydrolysis and enzyme concentration were optimized. the optimal enzymatic hydrolysis conditions were as follows: cellulose enzymes 0.8 g/100 g, hemicellulase 1.2 g/100 g, and lignin 0.2 g/100 g, ratio of liquid to material ( m L/g) 6∶ 1, p H value 4, extraction lasts for 3.5 h under the temperature of 40 ℃, the pectin gain rate can reach2.63% ± 0.021%.
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Keywords:
- Passionflower /
- pectin /
- enzyme /
- extraction
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[1] Dam S M, Nguyen D V.Optimization of pectin extraction from fruit peel of purple passion fruit (passiflora edulis sims) in Vietnam[J].Acta Horticulturae, 2013 (989) :279-284.
[2] 赵苹, 焦懿, 赵虹.西番莲的研究现状及在中国的利用前景[J].资源科学, 1999, 21 (3) :77-80. [3] 陈靖, 陈孔荣.果胶——一种有开发前途的药物制剂基质[J].现代应用药学, 1997 (3) :22-23. [4] Vorwerk S, Somerville S, Somerville C.The role of plant cell wall polysaccharide composition in disease resistance[J].Trends in Plant Science, 2004, 9 (4) :203-209.
[4] 段红, 曹稳根.果胶及其应用研究进展[J].宿州学院学报, 2006 (6) :80-83. [5] Endress H U.Nonfood Uses of Pectin[M].The Chemistry and Technology of Pectin, 1991:251-268.
[6] Gholamreza Mesbahi, Jalal Jamalian, Asgar Farahnaky.A comparative study on functional properties of beet and citrus pectins in food systems[J].Food Hydrocolloids, 2005, 19 (4) :731-738.
[7] 冯洪建, 黄国林.果胶提取的研究进展[J].广东化工, 2013, 40 (21) :84-85. [8] 黄永春, 马月飞, 谢清若, 等.超声波辅助提取西番莲果皮中果胶的研究[J].食品科学, 2006, 27 (10) :341-344. [9] 刘艳梅.超声波辅助提取西番莲果皮中果胶的研究[J].企业科技与发展, 2009 (16) :7-10. [10] Kulkarni S G, Vijayanand P.Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f.flavicarpa L.) [J].Lebensmittel-Wissenschaft und-Technologie, 2010, 43 (7) :1026-1031.
[11] 陈智毅, 李升峰, 吴继军, 等.西番莲的加工利用研究[J].现代食品科技, 2006 (1) :186-189. [12] 郑荣珍, 钟永聪, 叶海辉.从西番莲果皮中提取果胶[J].热带农业科学, 1998 (2) :32-35. [13] 赵利, 王杉.果胶的制备及其在食品工业的应用综述[J].食品科技, 1999 (5) :32-34. [14] 张燕, 毛桂枝, 刘蕴哲.离子交换树脂法提取桔皮中果胶[J].食品研究与开发, 2003, 24 (4) :52-54. [15] Fishman M L, Chau H K, Hoagland P, et al.Characterization of pectin, flash-extracted from orange albedo by microwave heating, under pressure[J].Carbohydrate Research, 2000, 323 (1-4) :126-138.
[16] 赵广河, 沈育林, 陈振林.酸法与酶法提取香蕉皮果胶工艺比较[J].广东农业科学, 2013 (12) :104-106. [17] 苏东林, 李高阳, 陈亮, 等.橘皮果胶生产工艺优化及品质分析[J].食品科学, 2011, 32 (18) :95-101. [18] 王卉, 黄朝晖, 徐云升, 等.香蕉皮果胶纤维素酶法提取工艺[J].农业工程, 2013, 4 (3) :74-76. [19] Zhang Q A, Zhang Z Q, Yue X F, et al.Response surface optimization of ultrasound-assisted oil extraction from autoclaved almond powder[J].Food Chemistry, 2009, 116 (2) :513-518.
[20] 张小玲.果胶的咔唑硫酸分光光度测定法研究[J].甘肃农业大学学报, 1999 (1) :75-78. [21] 刘义武, 孔昭华, 王碧, 等.酶法提取血橙皮果胶[J].食品研究与发, 2013 (24) :81-84. [22] Chou T D, Kokini J L.Rheological Properties and Conformation of Tomato Paste Pectins, Citrus and Apple Pectins[J].Journal of Food Science, 2006, 52 (6) :1658-1664.
[23] 张娜, 陈锦屏, 严静, 等.柿皮果胶纤维素酶法提取工艺[J].食品科学, 2011 (12) :160-163.
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