抑菌活性乳酸菌的筛选及其抑菌物质的提取条件优化

双全; 折米娜; 薄礼娟

doi:10.13386/j.issn1002-0306.2017.18.021

抑菌活性乳酸菌的筛选及其抑菌物质的提取条件优化

内蒙古农业大学食品科学与工程学院

基金项目:

国家自然科学基金(31460443)；内蒙古自治区自然科学基金项目(2016MS0338)；

详细信息

作者简介:
双全 (1964-) , 男, 博士, 教授, 研究方向:食品科学, E-mail:shungquan688@126.com。;

中图分类号: TS201.3
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出版历程
- 收稿日期: 2017-02-26

Screening of lactic acid bacteria with antibacterial activity and optimization of extraction conditions of antimicrobial substances

College of Food Science and Engineering, Inner Mongolia Agricultural University

摘要

摘要: 从内蒙古东部地区发酵酸菜中分离的乳酸菌为供试菌株,以大肠杆菌和金黄色葡萄球菌为指示菌进行了抑菌活性筛选,并检测其抑菌谱。通过单因素和L₉（3⁴）正交实验确定抑菌活性物质的最佳提取条件,并应用于市售牛饲料中检测其抑菌效果。结果表明,在发酵酸菜中分离的14株供试乳酸菌中获得了1株抑菌活性较强且稳定的菌株S1-4,该菌株对大肠杆菌和金黄色葡萄球菌的抑菌圈直径分别达（19.69±0.71）mm和（20.59±0.26）mm,并呈现出较广的抑菌谱。菌株S1-4产抑菌活性物质的最佳提取条件:沉淀p H为5.0,乙醇浓度95%,沉淀时间8 h,料液比为1∶4（v∶v）,在此条件下,抑菌圈直径为39.53 mm,提高了23.18%。菌株S1-4发酵液添加到牛饲料中,可抑制饲料中细菌和霉菌的生长,并且随添加量的增加,抑菌效果明显增加。
- 乳酸菌 /
- 抑菌活性 /
- 抑菌物质 /
- 提取 /
- 饲料
Abstract: Screening on lactic acid bacteria with antibacterial activity, isolated from fermented pickle in eastern Inner Mongolia, was tested for Escherichia coli and Staphylococcus aureus as the indicator, and its antibacterial spectrum was tested.The optimum separation conditions of antibacterial substances were determined by single factor and L₉ ( 3⁴) orthogonal test, and detected antibacterial effect in commercial cattle feed.The result showed that the strain S1-4 had strong and stable antibacterial activity in 14 strains of lactic acid bacteria, and the diameter of inhibition zone of strain S1-4 to Escherichia coli and Staphylococcus aureus was ( 19.69 ± 0.71) mm and ( 20.59 ± 0.26) mm, respectively, and showed broad antimicrobial spectrum. The optimal extraction conditions for the antibacterial substances from strain S1-4 were as follows: the precipitation of p H was 5, the concentration of ethanol was 95%, the settling time was 8 h, and the ratio of material to liquid was 1 ∶ 4 ( v ∶ v) . In these conditions, the diameter of the inhibition zone was 39.53 mm, and increased by 23.18%.The fermentation broth of strain S1-4was added to the cattle feed, which could inhibit the growth of bacteria and mould in feed, and with the increase of the dosage, the inhibitory effect increased obviously.
- lactic acid bacteria /
- antibacterial activity /
- antimicrobial substances /
- extract /
- feed

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