Catalytic synthesis of DHA-PC with high DHA contents by phospholipase A2 in supercritical CO2 system
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摘要: 以南极磷虾粉为原料,乙醇浸提后分离纯化得到的精制磷脂酰胆碱的纯度高达90.8%,以此为原料,在超临界CO2体系中使用磷脂酶A2（PLA2）催化磷脂酰胆碱（PC）Sn-2位脂肪酸水解,使游离DHA结合到Sn-2位以制备高DHA含量的磷脂酰胆碱（DHA-PC）,并研究了不同参数对该反应的影响。最佳酶解反应条件分别如下:固定化PLA2的添加量15%（占总底物）,时间6 h,温度55℃,压力12 MPa。在此条件下,南极磷虾磷脂酰胆碱上DHA的含量可达62.3 mol%。由此得出结论,PLA2用于磷脂酰胆碱改性以制备DHA-PC的可行性高。Abstract: With the raw material of euphausia superba powder, the purity of the refining phosphatidylcholine gained by ethyl alcohol extraction and separation and purification reached 90.8%, then the refining phosphatidylcholine was used as raw material and phospholipase A2 was used to catalyze the fatty acid hydrolysis of Sn-2 position of phosphatidylcholine in supercritical CO2 system to make the fish oil DHA to Sn-2 position and form the DHA-PC with high DHA contents.Meanwhile, the effects of different parameters on the reaction were surveyed.The results showed that, the best additive amount of immobilization phospholipase A2 was 15% ( occupied the total substratio) , the best enzymolysis was 6 h, the best enzymolysis temperature was 55 ℃, the best enzymolysis pressure was 12 MPa, respectively.Under such condition, the DHA binding ratio of euphausia superba phosphatidylcholine could reach 62.3 mol%. And it could be concluded that immobilization phospholipase A2 is highly viable for phosphatidylcholine modification to prepare DHA-PC.
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