• 中国科技期刊卓越行动计划项目资助期刊
  • 中国精品科技期刊
  • EI
  • Scopus
  • CAB Abstracts
  • Global Health
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国科技核心期刊CSTPCD
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国开放获取期刊数据库COAJ
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020

不同干燥方法对金针菇多糖抗氧化活性的影响

陈义勇, 李家祺, 赵圆圆, 陈慧, 顾冰飞, 李一天, 赵培

陈义勇, 李家祺, 赵圆圆, 陈慧, 顾冰飞, 李一天, 赵培. 不同干燥方法对金针菇多糖抗氧化活性的影响[J]. 食品工业科技, 2017, (18): 40-44. DOI: 10.13386/j.issn1002-0306.2017.18.008
引用本文: 陈义勇, 李家祺, 赵圆圆, 陈慧, 顾冰飞, 李一天, 赵培. 不同干燥方法对金针菇多糖抗氧化活性的影响[J]. 食品工业科技, 2017, (18): 40-44. DOI: 10.13386/j.issn1002-0306.2017.18.008
CHEN Yi-yong, LI Jia-qi, ZHAO Yuan-yuan, CHEN Hui, GU Bing-fei, LI Yi-tian, ZHAO Pei. Effect of different drying methods on antioxidant activities of polysaccharides extracted from Flammulina velutipes[J]. Science and Technology of Food Industry, 2017, (18): 40-44. DOI: 10.13386/j.issn1002-0306.2017.18.008
Citation: CHEN Yi-yong, LI Jia-qi, ZHAO Yuan-yuan, CHEN Hui, GU Bing-fei, LI Yi-tian, ZHAO Pei. Effect of different drying methods on antioxidant activities of polysaccharides extracted from Flammulina velutipes[J]. Science and Technology of Food Industry, 2017, (18): 40-44. DOI: 10.13386/j.issn1002-0306.2017.18.008

不同干燥方法对金针菇多糖抗氧化活性的影响

基金项目: 

国家星火计划项目(2014GA690011); 张家港市科技支撑计划(农业)项目(ZKN1304);

详细信息
    作者简介:

    陈义勇 (1974-) , 男, 博士, 副教授, 研究方向:食品科学与天然活性成分, E-mail:chenyyxp@126.com。;

  • 中图分类号: TS219

Effect of different drying methods on antioxidant activities of polysaccharides extracted from Flammulina velutipes

  • 摘要: 以金针菇为原料,利用传统水提法提取金针菇多糖(polysaccharides from Flammulina velutipes,FVP),分别对传统热风干燥多糖(FVP-H)、真空干燥多糖(FVP-V)和冷冻干燥多糖(FVP-F)的成分和抗氧化活性进行研究。结果表明,FVP为酸性多糖,不同干燥方法对其单糖种类没有影响,但会改变单糖的构成比例和糖醛酸含量,FVP-H、FVP-V和FVP-F相对分子质量变化不明显,分别为2342309、2375843和2358437 Da。FVP-H、FVP-V和FVP-F具有较强的清除DPPH自由基、羟基自由基、超氧阴离子自由基的能力和还原力,在一定浓度范围内,多糖与抗氧化活性及还原能力之间呈正相关关系,干燥方法对FVP抗氧化活性具有不同的影响,其中FVP-F抗氧化活性最强,其次是FVP-V和FVP-H,冷冻干燥是制备FVP适宜的干燥方法。 
    Abstract: Flammulina velutipes was used as raw materials. Polysaccharides from Flammulina velutipes ( FVP) were extracted using conventional water extraction.FVP-H, FVP-V and FVP-F were obtained with hot-air drying, vacuum drying and freeze drying, respectively.Component and antioxidant activities of FVP-H, FVP-V and FVP-F were investigated.The results showed that FVP were acidic polysaccharides.Different drying methods had no effect on the monosaccharide species. However, it could change the monosaccharide composition and uronic acid content.The relative molecular weight of FVP-H, FVP-V and FVP-F were not obvious, which were 2342309, 2375843 and 2358437 Da, respectively. FVP-H, FVP-V and FVP-F had a stronger scavenging ability of DPPH radical, hydroxyl radical, superoxide anion free radical and reducing power. In a certain concentration range, FVP were positively correlated with the antioxidant activity and reducing capacity.Different drying methods had different effects on the antioxidant activity of FVP. FVP-F had highest antioxidant activities, followed by FVP-V and FVP-H. Freeze-drying was the best drying method for preparation of FVP.
  • [1] 郑义, 李超, 王乃馨.金针菇多糖的研究进展[J].食品科学, 2010, 31 (17) :425-428.
    [2] 常花蕾, 雷林生, 余传林, 等.金针菇多糖对小鼠免疫细胞产生细胞因子及对荷瘤小鼠血清细胞因子含量的影响[J].中药材, 2009, 32 (4) :561-563.
    [3]

    Pang XB, Yao WB, Yang XB.Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium[J].Carbohydrate Polymers, 2007, 70 (3) :291-297.

    [4] 陈芝芸, 严茂祥, 项柏康.金针菇多糖对Lewis肺癌荷瘤小鼠的抑瘤作用及免疫功能的影响[J].中国中医药科技, 2003, 10 (4) :226-227.
    [5] 潘鸿辉, 宇雄涛, 黄纪国, 等.金针菇多糖改善小鼠学习记忆功能[J].中国食用菌, 2014, 35 (5) :40-42.
    [6] 徐洲, 刘静, 冯士令, 等.不同干燥方法对淫羊藿多糖化学性质和抗氧化活性的影响[J].食品工业科技, 2015, 36 (19) :116-119, 123.
    [7] 吴振, 李红, 罗杨, 等.不同干燥方式对银耳多糖理化特性及抗氧化活性的影响[J].食品科学, 2014, 35 (13) :93-97.
    [8]

    Fan LP, Li JW, Deng KQ, et al.Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum[J].Carbohydrate Polymers, 2012, 87 (2) :1849-1854.

    [9] 张惟杰主编.糖复合物生化研究技术[M].杭州:浙江大学出版社, 2003:52-54.
    [10]

    Lowry OH, Rosebrough NJ, Farr A, et al.Protein measurement with the folin phenol reagent[J].Journal of Biological Chemistry, 1951, 193:265-275.

    [11]

    Blumenkrantz N, Gasboe H.New method for quantitative determination of uronic acid[J].Aanalytical Biochemistry, 1973, 54:484-489.

    [12]

    Wang HY, Jiang XL, Mu HJ, et al.Structure and protective effect of exopolysaccharide from P.Agglomerans strain KFS-9against UV radiation[J].Microbiological Research, 2007, 162 (2) :124-129.

    [13]

    Liu LX, Sun Y, Laura T, et al.Determination of polyphenolic content and antioxidant activity of kudingcha made from Ilex kudingcha C.J.Tseng[J].Food Chemistry, 2009, 112 (1) :35-41.

    [14]

    Jiang B, Zhang HY, Liu CJ, et al.Extraction of watersoluble polysaccharide and the antioxidant activity from Ginkgo biloba leaves[J].Medicinal Chemistry Research, 2010, 19 (3) :262-270.

    [15]

    Wu HC, Chen HM, Shiau CY.Free amino acids and peptides as related to antioxidant properties in protein ydrolysates of mackerel (Scomber austriasicus) [J].Food Research International, 2003, 36 (9/10) :949-957.

    [16] 熊双丽, 李安林.酸性多糖的最新研究进展[J].食品科技, 2010, 35 (5) :80-83.
    [17]

    Ma LS, Chen HX, Zhu WC, et al.Effect of different drying methods on physicochemical properties and antioxidant activities of polysaccharides extracted from mushroom Inonotus obliquus[J].Food Research International, 2013, 50 (2) :633-640.

计量
  • 文章访问数:  192
  • HTML全文浏览量:  28
  • PDF下载量:  262
  • 被引次数: 0
出版历程
  • 收稿日期:  2017-02-06

目录

    /

    返回文章
    返回
    x 关闭 永久关闭