Effect of heat treatment on the microrheological properties of lactoferrin-β-carotene/soybean polysaccharide-DHA emulsion
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摘要: 蛋白质功能因子乳状液体系巴氏杀菌受热时易改变其流变性,从而影响其品质。本研究基于乳状液异型聚集效应,构建多重功能因子乳状液微聚集体,以期改善其耐热性。选用大豆多糖(SSPS)和乳铁蛋白(LF)为乳化剂,DHA和β-胡萝卜素为功能因子,分别制备了SSPS-DHA乳状液和LF-β-胡萝卜素乳状液,二者以不同比例异型聚集,形成不同的乳状液微聚集体。考察不同加热方式2580℃程序加热30 min(加热过程)和80℃加热30 min(模拟巴氏杀菌)对乳状液的物理稳定性与流变特性的影响。结果表明:单一LF-β-胡萝卜素乳状液加热后完全变性成凝胶状固体;SSPS-DHA/LF-β-胡萝卜素乳状液微聚集体在加热条件下,流变学特性明显改善;90 wt%LF-β-胡萝卜素-10 wt%SSPS-DHA乳状液微聚集体的流动性、粘弹性均表现为流体特征。因此,通过SSPS-DHA乳状液的添加可以显著改善蛋白乳状液的热变性与热凝固特性,从而保障巴氏杀菌等热处理不改变功能因子乳状液的质构。Abstract: The rheological properties of protein emulsion system was easy to be changed when it was heated, thus affecting its quality.In order to improve the heat resistance of the emulsion, a new multi-functional emulsion microaggregate was constructed based on the heteroaggregation effect of emulsion.Soybean polysaccharide ( SSPS) and lactoferrin ( LF) as emulsifier, DHA andβ-carotene as functional components were prepared to SSPS-DHA emulsion and LF-β-carotene emulsion. Then, heteroaggregation forming emulsions of different micro-clusters occurred in different proportions. The effects of 25 ~ 80 ℃programmed heating 30 min ( heating process) and 80 ℃ heating 30 min ( simulated pasteurization) on the physical stability and rheological properties of the emulsion were investigated. The results showed that the single LF-β-carotene emulsion was completely denatured and exhibited solid after heating.The rheological properties of the SSPS-DHA/LF-β-carotene emulsion micro-aggregates were significantly improved under heating condition, the fluidity and viscoelastic of the 90 wt% LF-β-carotene-10 wt% SSPS-DHA emulsion micro-aggregates showed fluid characteristics. Therefore, the addition of SSPS-DHA emulsion significantly improved the thermal denaturation and thermal coagulation characteristics of the protein emulsion, thus ensuring that the heat treatment of pasteurization did not change the texture of the functional compound emulsion.
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Keywords:
- lactoferrin (LF) /
- soybean polysaccharide (SSPS) /
- β-carotene /
- DHA /
- emulsions /
- thermal treatment /
- microrheological
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