• EI
  • Scopus
  • 中国科技期刊卓越行动计划项目资助期刊
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国精品科技期刊
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国科技核心期刊CSTPCD
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020

热处理对乳铁蛋白-β-胡萝卜素/大豆多糖-DHA乳状液微流变特性的影响

李昕, 王旭, 李玮克, 许朵霞, 曹雁平

李昕, 王旭, 李玮克, 许朵霞, 曹雁平. 热处理对乳铁蛋白-β-胡萝卜素/大豆多糖-DHA乳状液微流变特性的影响[J]. 食品工业科技, 2017, (18): 1-7. DOI: 10.13386/j.issn1002-0306.2017.18.001
引用本文: 李昕, 王旭, 李玮克, 许朵霞, 曹雁平. 热处理对乳铁蛋白-β-胡萝卜素/大豆多糖-DHA乳状液微流变特性的影响[J]. 食品工业科技, 2017, (18): 1-7. DOI: 10.13386/j.issn1002-0306.2017.18.001
LI Xin, WANG Xu, LI Wei-ke, XU Duo-xia, CAO Yan-ping. Effect of heat treatment on the microrheological properties of lactoferrin-β-carotene/soybean polysaccharide-DHA emulsion[J]. Science and Technology of Food Industry, 2017, (18): 1-7. DOI: 10.13386/j.issn1002-0306.2017.18.001
Citation: LI Xin, WANG Xu, LI Wei-ke, XU Duo-xia, CAO Yan-ping. Effect of heat treatment on the microrheological properties of lactoferrin-β-carotene/soybean polysaccharide-DHA emulsion[J]. Science and Technology of Food Industry, 2017, (18): 1-7. DOI: 10.13386/j.issn1002-0306.2017.18.001

热处理对乳铁蛋白-β-胡萝卜素/大豆多糖-DHA乳状液微流变特性的影响

基金项目: 

北京市自然科学基金三元联合资助项目(1550003); 国家自然科学基金项目(31501486); 十三五国家科技计划项目申报中心重点研发计划(2016YFD0400802); 北京市优秀人才培养资助项目(2014000020124G032); 北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20130506);

详细信息
    作者简介:

    李昕 (1993-) , 女, 硕士研究生, 研究方向:食品添加剂与功能性配料, E-mail:1016903505@qq.com。;

    许朵霞 (1986-) , 女, 博士, 副教授, 研究方向:食品添加剂与功能性配料, E-mail:xdxbtbu@126.com。;

    曹雁平 (1961-) , 男, 博士, 教授, 研究方向:食品化学与安全, E-mail:caoyp@btbu.edu.cn。;

  • 中图分类号: TS201.2

Effect of heat treatment on the microrheological properties of lactoferrin-β-carotene/soybean polysaccharide-DHA emulsion

  • 摘要: 蛋白质功能因子乳状液体系巴氏杀菌受热时易改变其流变性,从而影响其品质。本研究基于乳状液异型聚集效应,构建多重功能因子乳状液微聚集体,以期改善其耐热性。选用大豆多糖(SSPS)和乳铁蛋白(LF)为乳化剂,DHA和β-胡萝卜素为功能因子,分别制备了SSPS-DHA乳状液和LF-β-胡萝卜素乳状液,二者以不同比例异型聚集,形成不同的乳状液微聚集体。考察不同加热方式2580℃程序加热30 min(加热过程)和80℃加热30 min(模拟巴氏杀菌)对乳状液的物理稳定性与流变特性的影响。结果表明:单一LF-β-胡萝卜素乳状液加热后完全变性成凝胶状固体;SSPS-DHA/LF-β-胡萝卜素乳状液微聚集体在加热条件下,流变学特性明显改善;90 wt%LF-β-胡萝卜素-10 wt%SSPS-DHA乳状液微聚集体的流动性、粘弹性均表现为流体特征。因此,通过SSPS-DHA乳状液的添加可以显著改善蛋白乳状液的热变性与热凝固特性,从而保障巴氏杀菌等热处理不改变功能因子乳状液的质构。 
    Abstract: The rheological properties of protein emulsion system was easy to be changed when it was heated, thus affecting its quality.In order to improve the heat resistance of the emulsion, a new multi-functional emulsion microaggregate was constructed based on the heteroaggregation effect of emulsion.Soybean polysaccharide ( SSPS) and lactoferrin ( LF) as emulsifier, DHA andβ-carotene as functional components were prepared to SSPS-DHA emulsion and LF-β-carotene emulsion. Then, heteroaggregation forming emulsions of different micro-clusters occurred in different proportions. The effects of 25 ~ 80 ℃programmed heating 30 min ( heating process) and 80 ℃ heating 30 min ( simulated pasteurization) on the physical stability and rheological properties of the emulsion were investigated. The results showed that the single LF-β-carotene emulsion was completely denatured and exhibited solid after heating.The rheological properties of the SSPS-DHA/LF-β-carotene emulsion micro-aggregates were significantly improved under heating condition, the fluidity and viscoelastic of the 90 wt% LF-β-carotene-10 wt% SSPS-DHA emulsion micro-aggregates showed fluid characteristics. Therefore, the addition of SSPS-DHA emulsion significantly improved the thermal denaturation and thermal coagulation characteristics of the protein emulsion, thus ensuring that the heat treatment of pasteurization did not change the texture of the functional compound emulsion.
  • [1]

    Baker E N, Baker H M.Molecular structure, binding properties and dynamics of lactoferrin[J].Cellular and Molecular Life Sciences, 2005, 62 (22) :2531-2539.

    [2]

    Steijns J M, Van Hooijdonk A C M.Occurrence, structure, biochemical properties and technological characteristics of lactoferrin[J].British Journal of Nutrition, 2000, 84 (1) :11-17.

    [3]

    Tokle T, Mc Clements D E.Physicochemical properties of lactoferrin stabilized oil-in-water emulsions:Effects of p H, salt and heating[J].Food Hydrocolloids, 2011, 25 (5) :976-982.

    [4]

    Ye A, Singh H.Adsorption behaviour of lactoferrin in oil-inwater emulsions as influenced by interactions withβ-lactoglobulin[J].Journal of Colloid and Interface Science, 2006, 295 (1) :249-254.

    [5]

    Nakamura A, Yoshida R, Maeda H, et al.Soy soluble polysaccharide stabilization at oil-water interfaces[J].Food Hydrocolloids, 2006, 20:277-283.

    [6]

    Nakamura A, Yoshida R, Maeda H, et al.Study of the role of the carbohydrate and protein moieties of soy soluble polysaccharides in their emulsifying properties[J].Journal of Agricultural and Food Chemistry, 2004, 52:5506-5512.

    [7]

    Kiokias S, Gordon M H.Antioxidant properties of carotenoids in vitro and in vivo[J].Food Reviews International, 2014, 20:99-121.

    [8]

    Grabowski J A, Truong V D, Daubert C R.Nutritional and rheological characterization of spray dried sweetpotato powder[J].LWT-Food Science and Technology, 2008, 41:206-216.

    [9]

    Marty C, Berset C.Factors affecting the thermal degradation of all-transbeta-carotene[J].Journal of Agricultural and Food Chemistry, 1990, 38:1063-1067.

    [10]

    Ribeiro H S, Cruz R C D.Biliquid foams containing carotenoids[J].Engineering in Life Sciences, 2005, 5:84-88.

    [11] 许朵霞.乳清分离蛋白-甜菜果胶不同相互作用对β-胡萝卜素乳状液稳定机理及体外释放特性研究[D].北京:中国农业大学, 2012.
    [12]

    Henry L K, Puspitasari-Nienaber N L, Jaren-Galan M, et al.Effects of ozone and oxygen on the degradation of carotenoids in an aqueous model system[J].Journal of Agricultural and Food Chemistry, 2010, 48:5008-5013.

    [13]

    Rascon M P, Beristain C I, Garcia H S, et al.Carotenoid retention and storage stability of spray-dried encapsulated paprika oleoresin using gum Arabic and soy protein isolate as wall materials[J].LWT-Food Science and Technology, 2011, 44:549-557.

    [14]

    Alamed J, Mc Clements D E, Decker E A.Relationships between free radical scavenging and antioxidant activity in foods[J].Food Chemistry, 2009, 57:2969-2976.

    [15]

    Tanmoy K D, Santinath G, Mahua G, et al.Comparative study of gastrointestinal absorption of EPA&DHA rich fish oil from nano and conventional emulsion formulation in rats[J].Food Research International, 2012 (49) :72-79.

    [16]

    Leclerc L, Zhao X, Simpson B K, et al.The effect of a-tocopherol on the oxidation of mackerel oil[J].Food Chemistry, 2007, 100:800-807.

    [17]

    Salariya A M, Rehman Z.Effect of synthetic antioxidants on storage stability of Khoa-asemi-solid concentrated milk product[J].Food Chemistry, 2006, 96:122-125.

    [18] 毛立科.β-胡萝卜素纳米乳液的制备及其理化性质研究[D].北京:中国农业大学, 2008.
    [19]

    Liu F, Wang D, Sun C.Influence of polysaccharides on the physicochemical properties of lactoferrin-polyphenol conjugates coatedβ-carotene emulsions[J].Food Hydrocolloids, 2016, 52:661-669.

    [20] 梁蓉.以OSA变性淀粉为乳化剂的纳米乳液制备及特性研究[D].无锡:江南大学, 2013.
    [21]

    Bouyer E, Mekhloufi G, Le Potier I, et al.Stabilization mechanism of oil-in-water emulsions byβ-lactoglobulin and gum Arabic[J].Journal of Colloid and Interface Science, 2011, 354 (2) :467-477.

    [22]

    Guzey D, Mc Clements D E.Formation, stability and properties of multilayer emulsions for application in the food industry[J].Advances in Colloid and Interface Science, 2006, 128:227-248.

    [23]

    Yang N, Hutter J L, Bruyn J R.Microrheology, microstructure and aging of physically cross-linked poly (vinyl alcohol) /poly (ethylene glycol) blends[J].Journal of Rheology, 2012, 56:797-822.

    [24]

    Dickinson E.Hydrocolloids as emulsifiers and emulsion stabilizers[J].Food Hydrocolloids, 2009, 23 (6) :1473-1482.

    [25]

    Mason T.Particle tracking microrheology of complex fluids[J].Physical Review Letters, 2007, 79:3282-3285.

    [26]

    Ambrosio L.Rheological study on hyaluronic acid and its derivative solutions[J].Journal of Macromolecular Science, 2009, 36:991-1000.

  • 期刊类型引用(3)

    1. 胡智恺,索一平,李爽,王雨婷,刘薇,史锦硕,杨霞,姜洁. 婴儿配方奶粉中克罗诺杆菌的快速检测. 中国酿造. 2023(08): 253-259 . 百度学术
    2. 丁伯乐,蔡为荣,闻志莹,岳丹伟,朱樱,李晶晶. 山药低聚糖制备分离及对五种益生菌的增殖作用. 食品与发酵工业. 2020(24): 74-79 . 百度学术
    3. 李婧,张柏林. 低聚葡萄糖对益生菌的增殖影响. 宁夏农林科技. 2019(12): 72-74+84 . 百度学术

    其他类型引用(3)

计量
  • 文章访问数:  261
  • HTML全文浏览量:  41
  • PDF下载量:  302
  • 被引次数: 6
出版历程
  • 收稿日期:  2017-03-19

目录

    /

    返回文章
    返回
    x 关闭 永久关闭