基于近红外光谱定量分析牛奶中掺假的动物水解蛋白含量

范睿; 孙晓凯; 杨晨; 陈杰; 刘东武; 孔玲; 陈志伟

doi:10.13386/j.issn1002-0306.2017.16.048

基于近红外光谱定量分析牛奶中掺假的动物水解蛋白含量

1. 山东理工大学生命科学学院
2. 山东理工大学分析测试中心

基金项目:

国家自然科学基金面上项目(31071538)；山东省自然科学基金重点项目(ZR2013FB001)；山东省大型科学仪器升级改造项目(2011SJGZ10)；

详细信息

作者简介:
范睿 (1992-) , 男, 硕士研究生, 研究方向:生物分析化学, E-mail:fan_rui@126.com。;

孔玲 (1974-) , 女, 博士, 副教授, 研究方向:生物分析、生物化工, E-mail:klucy@sdut.edu.cn。;

陈志伟 (1972-) , 男, 博士, 教授, 研究方向:生物分析化学、生物统计学, E-mail:chen@sdut.edu.cn。;

中图分类号: O657.33;TS252.7
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出版历程
- 收稿日期: 2017-01-08

Quantitative analysis of hydrolysate animal protein ( HAP) adulteration in milk by near infrared spectroscopy

School of Life and Science, Shandong University of Technology
Analysis and Test Center, Shandong University of Technology

摘要

摘要: 动物水解蛋白含有大量重金属离子,摄入人体危害人体健康,因其蛋白质含量高,被不法商贩作为廉价蛋白质添加在牛奶中以提高蛋白质含量。本研究自行配制不同浓度的动物水解蛋白掺假牛奶,应用近红外光谱仪浸入式光纤扫描样品,考察建模方法、预处理方法、建模范围、主成分数及样品的异常点分析对定量分析模型的影响。结果表明:使用SG平滑处理、主成分数4、在10000⁵000 cm^-1使用PLS法建立了最优动物水解蛋白定量分析模型。该模型的校正均方差（RMSEC）、预测均方差（RMSEP）、相关系数（r）分别为0.115、0.305、0.983,平均回收率为104.44%。该分析模型可以用于牛奶中动物水解蛋白的快速定量分析,为牛奶的品质控制和快速定量提供参考。
- 牛奶掺假 /
- 动物水解蛋白 /
- 定量分析 /
- 近红外光谱
Abstract: Hydrolysate animal protein ( HAP) contains a lot of heavy metal ions which are harmful to human health. Due to its high contents of protein, HAP is used to improve the protein content in milk as an inexpensive source of protein.In this study, milk samples adulterated with different gradients of HAP were prepared. Then, the samples were scanned using near-infrared spectrometer with Fiber Optic Module and the factors influencing quantitative analysis models such as modeling methods, pretreatment methods, principal components and abnormal points were investigated with TQ software.The results indicated that the root mean square error of cross ( RMSEC) , the root mean square error of prediction ( RMSEP) and the correlation coefficient ( r) of the established model was 0.115, 0.305 and 0.983 respectively, the recovery was 104.44%.The model could be used in rapid quantitative analysis of HAP in milk.
- adulterated milk /
- hydrolysate animal protein (HAP) /
- quantitative analysis /
- near infrared spectroscopy

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参考文献(19)

[1]	刘金源, 骆庆.19世纪伦敦市场上的牛奶掺假问题[J].世界历史, 2014 (1) :66-75, 160.
[2]	陶跃华, 张晓峰.从“三鹿奶粉事件”浅析我国食品安全监管现状及对策[J].中国卫生监督杂志, 2010 (4) :362-365.
[3]	陆东林, 张丹凤, 荆文清, 等.牛奶中的氨基酸含量及其营养价值[J].新疆畜牧业, 2001 (4) :12-14.
[4]	钱平, 孙国琴, 张存洲.基于近红外光谱技术的石油组分定量分析新方法[J].光谱学与光谱分析, 2008, 28 (12) :2851
[5]	Mauer L J, Chernyshova A A, Hiatt A, et al.Melamine detection in infant formula powder using near-and mid-infrared spectroscopy[J].J Agric Food Chem, 2009, 57:3974-3980.
[6]	刘莹, 傅泽田, 侯彩云等.人为添加三聚氰胺液态乳中蛋白质含量测定方法的研究[J].中国农业大学学报, 2009, 14 (2) :22-26.
[7]	Song H X Xue H Y, Han Y.Detection of cow’s milk in Shaanxi goat’s milk with an ELISA assay[J].Food Control, 2011, (22) :883-887.
[8]	Kotowicz M, Adamczyk E, Bania J.Application of a duplexPCR for detection of cow’s milk in goats milk[J].Ann Agric Environ Med, 2007, 14 (2) :215-218.
[9]	由丹青.纯牛奶中动物水解蛋白L-羟脯氨酸含量测定[J].中国奶牛, 2014 (Z2) :48-50.
[10]	刘婷, 姜金斗, 刘宁.HPLC-OPA柱后衍生离子交换法对乳粉中掺水解动物蛋白检测方法的研究[J].食品工业科技, 2007 (7) :207-209, 239.
[11]	赖玉婷, 董芷呈, 陈挺强, 等.基于氨基酸模式的牛奶蛋白掺假分析[J].食品工业科技, 2013, 34 (6) :82-84, 103.
[12]	Balabin R M, Smirnov S V.Melamine detection by mid-and near-in-frared (MIR/NIR) spectroscopy:a quick and sensitive method for dairyproducts analysis including liquid milk, infant formula, and milk powder[J].Talanta, 2011, 85 (1) :562-568.
[13]	Balabin R M, Safieva R Z, Lomakina E I.Near-infrared (NIR) spectroscopy for motor oil classification:From discriminant analysis to support vector machines[J].Microchemical Journal, 2011, 98 (1) :121-128.
[14]	Zhou X B, Zhao J W N, Povey M J W, et al.Variables selection methods in near-infrared spectroscopy[J].Analytica Chimica Acta, 2010, 667 (1-2) :14-32.
[15]	褚小立.近红外光谱分析技术使用手册[M].北京:机械工业出版社, 2016.
[16]	Liu C, Yang S X, Deng L.Determination of internal qualities of Newhall navel oranges based on NIR spectroscopy using machine learning[J].Journal of Food Engineering, 2015, 161:16-23.
[17]	Park B, Abbott J A, Lee, K J, et al.Near-infrared diffuse reflectance for quantitative and qualitative measurement of soluble solids and firmness of delicious and gala apples[J].Transactions of the Asae, 2003, 46 (6) :1721-1731.
[18]	胡小莉, 白雁, 雷敬卫, 等.NIRS结合TQ软件对不同产地野菊花定性定量分析[J].中国实验方剂学杂志, 2016 (15) :37-41.
[19]	Nas T, Isaksson T, Fearn T, et al.A user-friendly guide to multivariate calibration and classification[M].NIR Publications, 2002.

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