Quantitative analysis of hydrolysate animal protein ( HAP) adulteration in milk by near infrared spectroscopy
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摘要: 动物水解蛋白含有大量重金属离子,摄入人体危害人体健康,因其蛋白质含量高,被不法商贩作为廉价蛋白质添加在牛奶中以提高蛋白质含量。本研究自行配制不同浓度的动物水解蛋白掺假牛奶,应用近红外光谱仪浸入式光纤扫描样品,考察建模方法、预处理方法、建模范围、主成分数及样品的异常点分析对定量分析模型的影响。结果表明:使用SG平滑处理、主成分数4、在100005000 cm-1使用PLS法建立了最优动物水解蛋白定量分析模型。该模型的校正均方差（RMSEC）、预测均方差（RMSEP）、相关系数（r）分别为0.115、0.305、0.983,平均回收率为104.44%。该分析模型可以用于牛奶中动物水解蛋白的快速定量分析,为牛奶的品质控制和快速定量提供参考。Abstract: Hydrolysate animal protein ( HAP) contains a lot of heavy metal ions which are harmful to human health. Due to its high contents of protein, HAP is used to improve the protein content in milk as an inexpensive source of protein.In this study, milk samples adulterated with different gradients of HAP were prepared. Then, the samples were scanned using near-infrared spectrometer with Fiber Optic Module and the factors influencing quantitative analysis models such as modeling methods, pretreatment methods, principal components and abnormal points were investigated with TQ software.The results indicated that the root mean square error of cross ( RMSEC) , the root mean square error of prediction ( RMSEP) and the correlation coefficient ( r) of the established model was 0.115, 0.305 and 0.983 respectively, the recovery was 104.44%.The model could be used in rapid quantitative analysis of HAP in milk.
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