Optimization of ultrasonic extraction of proanthocyanidins from egg jujube by response surface methodology
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摘要: 本文采用单因素实验和响应面分析法对鸡蛋枣中原花青素进行提取工艺优化。在单因素实验的基础上,根据中心组合（BOX-Behnken）实验设计,采用四因素三水平对各个因素进行研究与分析,结果表明:鸡蛋枣中原花青素的最佳提取工艺为超声时间16 min、超声功率为618 W、超声温度为65℃、料液比为1∶21,在此工艺下的鸡蛋枣原花青素得率1.82%。证实超声波提取鸡蛋枣中原花青素的工艺是可行的。体外抗氧化实验表明:鸡蛋枣原花青素对DPPH自由基、羟基自由基的清除具有显著效果,同时还原力显著,且不输于VC。Abstract: The extraction of proanthocyanidins from egg jujube was investigated by the single experiments and response surface methodology.On the basis of single factor tests, the method of the central composite experimental design ( BOX-Behnken) was used to optimal of ultrasound-assisted extraction of proanthocyanidins form egg jujube by factors and three levels.The results indicated that the extractive conditions were optimized as follows: ultrasonic treatment time of 16 min, ultrasonic power of 618 W, ultrasonic temperature of 65 ℃ and the ratio of material to solvent of 1 ∶ 21 ( g/m L) .Under these conditions, the yield of Procyanidins from egg jujube was 1.82%. Antioxidant activities in egg jujube of the proanthocyanidins indicates that proanthocyandins of egg jujube had strong scavenging effects against DPPH radicals, ·OH radicals and reductiion force was significant, it was not lower than that of VC.
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Keywords:
- egg jujube /
- proanthocyanidins /
- the extraction rate /
- ultrasonic /
- response surface methodolgy
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[1] 郭盛, 段金廒, 唐于平, 等.中国枣属药用植物资源化学研究进展[J].中国现代中药, 2012, 14 (8) :1-5. [2] 丁胜华, 王蓉蓉, 吴继红, 等.枣果实中生物活性成分与生物活性的研究进展[J].现代食品科技, 2016 (5) :332-348. [3] 李勇革.发展“鸡蛋枣”前景光明[J].农家科技, 2001 (2) :41-42. [4] 李一凡, 王凤玲, 唐艳冲.响应面法优化超声波辅助提取玫瑰香葡萄中芪化物的工艺条件[J].食品工业科技, 2016, 37 (20) :258-262. [5] 孙智达, 石翠芳, 杨尔宁, 等.沙枣果肉原花青素的提取、纯化及清除·OH能力初探[J].食品工业科技, 2006 (9) :88-90. [6] Xiao L X, Zhi-Gui H E, Zhu Y, et al.Optimization of Proanthocyanidin Extraction from Grape Seed[J].Food Science, 2011, 32 (20) :89-94.
[7] 李佳桥, 余修亮, 曾林晖, 等.响应面实验优化超声波辅助提取莲房原花青素工艺[J].食品科学, 2016, 37 (12) :40-45. [8] 徐亚维, 赵杨.山东小枣中原花青素的提取及纯化研究[J].江苏农业科学, 2010 (6) :468-470. [9] 张娜, 雷芳, 马娇, 等.蒸制对红枣主要活性成分的影响[J].食品工业, 2017 (1) :138-141. [10] 游凤, 黄立新, 张彩虹, 等.冬枣各成熟阶段果皮酚类含量变化及其对DPPH自由基清除能力的影响[J].食品科学, 2013, 34 (19) :62-66. [11] 李华, 肖付才, 袁春龙, 等.铁盐催化比色法测定葡萄籽超微粉中的原花青素[J].食品研究与开发, 2007, 28 (9) :114-117. [12] 吴小燕, 王仁才, 石浩, 等.小米枣黄酮、多糖及其复配物体外抗氧化性评价[J].中南药学, 2016 (10) :1070-1074. [13] 张艳, 段雪芹, 高刚, 等.响应面法优化姜黄素的提取工艺及其抗氧化活性的研究[J].食品工业科技, 2015, 36 (6) :269-273. [14] 朱丽蓉, 吴萍萍, 杨大伟, 等.杜仲雄花茶多糖的响应面优化提取及其抗氧化活性评价[J].食品工业科技, 2015, 36 (3) :199-203. [15] Guo Y, Guo L, Qilin H U.Optimization of Extraction Process Proanthocyanidins Ultrasonic Method with Response Surface Methodology[J].Journal of Shanxi University, 2015.
[16] Liu X.Ultrasonic Extraction of Proanthocyanidins from SEMEN LITCHI[J].Jornal of Anh Agrlral N, 2011.
[17] 郑洪亮, 何飞, 腾飞, 等.红皮云杉球果原花青素提取优化及抗氧化活性评价[J].食品工业科技, 2014, 35 (10) :258-263. [18] Zhang Y.Study on Ultrasonic Extraction and Ultrafiltration Membrane Separation of Pine Bark Proanthocyanidins[J].Forestry Science&Technology, 2013.
[19] 刘新, 陈卫中, 韩琴.响应面法优化超声波辅助提取芒果核中原花青素的研究[J].食品工业科技, 2012, 33 (3) :227-231.
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