Intervention effects and mechanism research of mulberry crude extract on nonylphenol anxiety behavior of SD rats induced by nonylphenol
-
摘要: 目的:探讨桑葚粗提液对壬基酚染毒大鼠焦虑行为的干预效果。方法:48只SPF级SD大鼠随机分为正常对照组（玉米油）、桑葚对照组（桑葚粗提液,按多酚含量计,120 mg/kg）、壬基酚染毒组（270 mg/kg）、桑葚粗提液低、中、高剂量干预组（壬基酚灌胃剂量均为270 mg/kg,桑葚粗提液干预剂量分别为30、60和120 mg/kg）,利用旷场实验（OFT）和高架十字迷宫（EPM）来观察桑葚粗提液对壬基酚染毒大鼠焦虑行为的影响,并用ELISA试剂盒检测单胺类神经递质5-羟色胺、多巴胺、去甲肾上腺素水平和单胺氧化酶活性。结果表明,高剂量干预组大鼠在旷场实验中进入中央区域距离、进入中央区域的次数以及在高架十字迷宫实验中进入开臂时间百分比和进入开臂次数百分比均显著高于染毒组（p<0.05）。与壬基酚染毒组相比,高剂量干预组大鼠下丘脑5-羟色胺和多巴胺水平,中剂量干预组大鼠下丘脑多巴胺水平显著下调（p<0.05）,而且高剂量干预组还明显上调单胺氧化酶的活性（p<0.05）。同时随着干预剂量的增加,去甲肾上腺素水平逐渐降低但并无显著性差异。结论:桑葚粗提液降低壬基酚所致焦虑大鼠下丘脑5-羟色胺、多巴胺、去甲肾上腺素水平,其作用机制可能与上调下丘脑单胺氧化酶的活性有关。Abstract: The aim of this study was toinvestigate the interventioneffects of mulberry crude extract ( MCE) on anxiety behaviors of SD rats exposed to nonylphenol ( NP) .48 SPF rats were randomly divided into 6 goups: normal control group ( corn oil) , MCE control group ( 120 mg/kg) , NP model group ( 270 mg/kg) , and three intervention groups ( 120 mg/kg NP + 30 mg/kg MCE, 120 mg/kg NP + 60 mg/kg MCE, 120 mg/kg NP + 120 mg/kg MCE) .The open field test ( OFT) and elevated plus maze ( EPM) were used to evaluate anxiety behaviors of the rats while the ELISA kitswere used to detect the levels of monoamine neurotransmitter, including serotonin, dopamine, norepinephrine and monoamine oxidase.The distance traveled in the center and entrance frequency to the center of the high-dose intervention groupwere significantly higher thanthe NP group in the OFT.The percentage of open arm entries and time of the high-dose intervention group rats were significantly higher thanthe NP group in the EPM test ( p < 0.05) .Compared with the nonylphenol group, the hypothalamic serotoninand dopamin levels in the high-dose intervention group were significantly lower ( p < 0.05) , the hypothalamus dopamin level was significantly lower in the middledose intervention group ( p < 0.05) and MAO activity of high dose intervention group was significantly increased ( p < 0.05) .Meanwhile as intervention dose increased, norepinephrine levels gradually reduce with no significant difference.Mulberry crude extract can reduce the levels of serotonin, dopamine, norepinephrine in hypothalamus of rats with anxiety caused by nonylphenol and the underlying mechanism may involve up-regulation of MAO activity.
-
[1] Andrew M N, Dunstan R H, O’Connor W A, et al.Effects of4-nonylphenol and 17alpha-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata:Vitellogenin induction and gonadal development[J].Aquatic toxicology (Amsterdam, Netherlands) , 2008, 88 (1) :39.
[2] 张诺, 贾瑞宝, 孙韶华, 等.壬基酚的检测及毒理学研究进展[J].环境与健康杂志, 2013, 30 (4) :362-365. [3] Mao Z, Zheng X F, Zhang Y Q, et al.Occurrence and Biodegradation of Nonylphenol in the Environment[J].International Journal of Molecular Sciences, 2012, 13 (1) :491-505.
[4] Chen W L, Wang G S, Gwo J C, et al.Ultra-high performance liquid chromatography/tandem mass spectrometry determination of feminizing chemicals in river water, sediment and tissue pretreated using disk-type solid-phase extraction and matrix solid-phase dispersion.[J].Talanta, 2012, 89 (2) :237-245.
[5] Liu J, Wang R, Huang B, et al.Distribution and bioaccumulation of steroidal and phenolic endocrine disrupting chemicals in wild fish species from Dianchi Lake, China[J].Environmental Pollution, 2011, 159 (10) :2815-2822.
[6] Xu E G B, Brian M, Lee J H W, et al.Environmental fate and ecological risks of nonylphenols and bisphenol A in the Cape D’Aguilar Marine Reserve, Hong Kong.[J].Marine Pollution Bulletin, 2015, 91 (1) :128-138.
[7] 李祥军.壬基酚和β-萘黄酮对河川沙塘鳢 (Odontobulis potamophila) 幼鱼雌激素/抗雌激素效应的研究[D].上海:华东师范大学, 2008. [8] Nagao T, Wada K, Marumo H, et al.Reproductive effects of nonylphenol in rats after gavage administration:a two-generation study[J].Reproductive Toxicology, 2001, 15 (3) :293-315.
[9] 张琼宇, 孙远东, 王中军, 等.壬基酚对金鱼 (Carassiusauratus) 胚胎发育毒性的初步研究[J].现代生物医学进展, 2016, 16 (16) :3040-3043. [10] 赖玉婷, 欧阳俊彦, 郭佑廷, 等.壬基酚对大鼠5-羟色胺分解代谢通路的影响[J].中国环境科学, 2014, 34 (9) :2408-2412. [11] 刘辉, 郭佑廷, 黄少文, 等.γ-氨基丁酸对DEHP染毒大鼠焦虑行为的干预研究[J].食品工业科技, 2015 (20) :354-358. [12] Yadav A V, Kawale L A, Nade V S.Effect of Morus alba L. (mulberry) leaves on anxiety in mice.[J].Indian Journal of Pharmacology, 2008, 40 (1) :32-36.
[13] Abbas B, Ahmad J J, Hasan A, et al.Effects of the Alcoholic Extract of White Mulberry Leaves on Behavioral Performance of Rats[J].Biomedical&Pharmacology Journal, 2015, 8:715-719.
[14] Shin W H, Park S J, Kim E J.Protective effect of anthocyanins in middle cerebral artery occlusion and reperfusion model of cerebral ischemia in rats[J].Life Sciences, 2006, 79 (2) :130-137.
[15] Kim A J, Park S.Mulberry extract supplements ameliorate the inflammation-related hematological parameters in carrageenaninduced arthritic rats[J].Journal of Medicinal Food, 2006, 9 (3) :431-435.
[16] Kim H G, Ju M S, Shim J S, et al.Mulberry fruit protects dopaminergic neurons in toxin-induced Parkinson’s disease models[J].British Journal of Nutrition, 2010, 104 (1) :8-16.
[17] 郑芳昊, 罗佳波.桂枝对大鼠中枢神经系统作用的研究[J].中药药理与临床, 2014, 30 (4) :76-79. [18] 范兴文, 贯士阔, 吴开良.脑部照射对大鼠情绪和记忆的影响[J].中国癌症杂志, 2014, 24 (11) :814-819. [19] 王维刚, 吴文婷, 周嘉斌, 等.应用高架十字迷宫分析小鼠焦虑行为[J].中国细胞生物学学报, 2011, 33 (5) :466-472. [20] 胡金奎.桑葚花色苷的分离制备, 结构分析及其体外活性[D].无锡:江南大学, 2013. [21] 娄在祥, 王洪新, 吕文平, 等.微波辅助提取牛蒡叶多酚及其抗氧化, 抗菌活性研究[J].食品与发酵工业, 2010, 36 (1) :161-165. [22] 肖更生, 徐玉娟, 刘学铭, 等.桑椹的营养、保健功能及其加工利用[J].中药材, 2001, 24 (1) :70-72. [23] 杨海霞, 朱祥瑞, 房泽民.桑椹的药用价值与开发利用[J].蚕桑通报, 2003, 34 (3) :5-8. [24] 张志强, 杨清香, 孙来华.桑葚的开发及利用现状[J].中国食品添加剂, 2009 (4) :65-68. [25] 董芳妮.长期双酚A暴露对成年小鼠焦虑和抑郁行为的影响及其神经机制[D].金华:浙江师范大学, 2014. [26] Xu Y, Wang Z, You W, et al.Antidepressant-like effect of trans-resveratrol:Involvement of serotonin and noradrenaline system[J].European Neuropsychopharmacology, 2010, 20 (6) :405-413.
[27] 刘露.芦丁预处理对TMT致学习记忆功能障碍的保护作用与单胺类神经递质及其代谢物的关系[D].重庆:重庆医科大学, 2014. [28] 刘倩佟.合欢花抗焦虑活性物质基础研究[D].北京:北京中医药大学, 2015. [29] Liu H, Yang J, Huang S, et al.Mulberry crude extracts induce Nrf2 activation and expression of detoxifying enzymes in rat liver:Implication for its protection against NP-induced toxic effects[J].Journal of Functional Foods, 2017, 32:367-374.
[30] 郑祖艳, 朱崇霞.焦虑症患者5-羟色胺及去甲肾上腺素水平的动态研究[J].福建中医药, 2004, 35 (2) :5. [31] 周奇志, 余曙光, 吴俊梅, 等.针刺对吗啡戒断后焦虑模型小鼠行为和脑内去甲肾上腺素含量的影响[J].成都中医药大学学报, 2003, 26 (1) :40-42. [32] 喻东山, 梅安昌.惊恐障碍的发生机制[J].四川精神卫生, 2009, 22 (2) :122-124. [33] 黄艳红, 辛晓燕, 陈亚琼, 等.卵巢切除及雌激素替代对大鼠脑单胺氧化酶和行为学的影响[J].中国行为医学科学, 2004, 13 (2) :140-141.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
