Lipid-lowering and anti-oxidative effects of curcumin on Caenorhabditis elegans
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摘要: 本文研究姜黄素对秀丽隐杆线虫的降脂及抗氧化作用。将L1期秀丽隐杆线虫暴露于不同浓度姜黄素（12.5、25、50和100μmol/L）48 h后,以油红O染色线虫肠道脂肪,检测线虫体内甘油三酯的含量变化,同时测定线虫体内总超氧化物歧化酶活力（T-SOD）和丙二醛（MDA）含量的变化。结果表明,与二甲基亚砜（DMSO）对照组相比,姜黄素浓度在25μmol/L以上显著降低线虫肠道脂肪（p<0.05）,姜黄素浓度在50μmol/L以上降低线虫肠道脂肪作用极显著（p<0.01）,随着姜黄素浓度升高,线虫肠道脂肪含量逐渐降低。姜黄素浓度在25μmol/L以上极显著降低线虫体内甘油三酯和MDA含量（p<0.01）。黄素浓度在50μmol/L以上极显著提高线虫体内T-SOD酶活力（p<0.01）。综上所述,姜黄素浓度在25μmol/L以上对线虫具有降脂及抗氧化保护作用。Abstract: To study the lipid-lowering and anti-oxidative effects of curcumin on Caenorhabditis elegans, C.elegans in L1 period were exposed to the different concentrations of curcumin ( 12.5, 25, 50 and 100 μmol/L) for 48 h. Oil red O stained nematode intestinal fat, the activities of T-SOD, the contents of MDA and triglyceride in C.elegans were measured. Results showed that compared with DMSO control group, curcumin could reduce intestinal fat in C.elegans at 25 μmol/L or more ( p < 0.05) , curcumin could reduce intestinal fat significantly at 50 μmol/L or more ( p < 0.01) , with the increasing of curcumin concentration, the content of intestinal fat decreased gradually.Curcumin could reduce the contents of triglyceride and MDA in C.elegans significantly at 25 μmol/L or more ( p < 0.01) . Curcumin could increase the activities of T-SOD in C.elegans significantly at 50 μmol/L or more ( p < 0.01) . In summary, curcumin had lipid-lowering and antioxidant protection effects at25 μmol/L or more.
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Keywords:
- curcumin /
- Caenorhabditis elegans /
- fat deposition /
- triglyceride /
- anti-oxidative
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