Antioxidative effect of α-tocopherol on the crab hepatopancreas
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摘要: 采用Schall烘箱加速氧化法,以空白和添加BHT处理为对照,研究不同浓度(0.01%、0.015%、0.02%)的α-生育酚和0.02%抗坏血酸增效剂复配对河蟹蟹黄油置于(90±1)℃的烘箱中的抗氧化效果。结果表明,随着贮藏时间的延长,当α-生育酚添加量为0.015%时,MDA值和POV值增长较为缓慢,贮藏结束后分别为270.375 nmol/m L、15.975 meq/kg,抑制蟹黄油氧化效果最佳;0.02%抗坏血酸与0.015%α-生育酚复配组的MDA值和POV值增长相对缓慢,且与添加BHT组无显著差异(p>0.05),贮藏结束后MDA值为202.5 nmol/m L,POV值为8.052 meq/kg,则抗坏血酸对α-生育酚的增效作用显著(p<0.05),添加增效剂的抗氧化效果明显优于单一天然抗氧化剂,且与合成抗氧化剂BHT抗氧化效果相当。GC-MS检测结果表明抗氧化剂处理对不饱和脂肪酸的氧化或分解保护作用显著,而添加0.02%抗坏血酸与0.015%α-生育酚复配的样品的不饱和脂肪酸含量为78.80%,而添加BHT样品的为78.97%,因此,抗氧化效果最优的是0.015%生育酚+0.02%抗坏血酸。Abstract: The antioxidative effect of α-tocopherol and related synergistic agent on the crab hepatopancreas was evaluated in this paper through Schall oven oxidation at ( 90 ± 1) ℃.α-tocopherol at different concentrations, 0.01%, 0.015% and 0.02%, as well as synergistic agent ascorbic acid, were used, compared with blank sample and sample added with BHT. The result showed that when the amount of α-tocopherol was 0.015%, the MDA value and POV value increased more slowly with the prolongation of storage time.After storage, the MDA value was 270.375 nmol/m L and the POV value was 15.975 meq/kg respectively.And 0.015% α-tocopherol had the best antioxidant effect on the crab hepatopancreas. The MDA value and POV value of 0.015% α-tocopherol and 0.02% ascorbic acid increased more slowly, which were not significantly different from those of BHT ( p > 0.05) .After storage, the MDA value was 202.5 nmol/m L and the POV value was 8.052 meq/kg respectively.Ascorbic acid had a significant synergistic effect with α-tocopherol ( p < 0.05) , showing better antioxidant activity than the single antioxidant α-tocopherol, which was equivalent with synthetic antioxidant BHT.GC-MS analysis showed that antioxidant treatment had significant protective effect on the oxidation or decomposition of unsaturated fatty acid. While unsaturated fatty acid content of the addition of 0.015% α-tocopherol and 0.02% ascorbic acid compound sample was 78.80%, and the addition of BHT sample was 78.97%.Therefore the optimized antioxidant formula was 0.015% α-tocopherol combined with 0.02%ascorbic acid.
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Keywords:
- crab hepatopancreas /
- α-tocopherol /
- ascorbic acid /
- antioxidation
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[1] 车斌, 王倩倩.江苏省河蟹养殖产业SWOT分析及对策[J].山西农业科学, 2011, 39 (7) :736-739. [2] 邵利平, 夏文水, 姜启兴, 等.蟹黄油的理化性质及其营养功能成分分析[J].食品工业科技, 2015, 36 (4) :362-369. [3] Calder P C.Marine omega-3 fatty acids and inflammatory processes:Effects, mechanisms and clinical relevance[J].Biochimica Et Biophysica Acta, 2014, 1851 (4) :469-484.
[4] Berge K, Musaveloso K, Harwood M, et al.Krill oil supplementation lowers serum triglycerides without increasing low-density lipoprotein cholesterol in adults with borderline high or high triglyceride levels[J].Nutrition Research, 2014, 34 (2) :126-133.
[5] 武华, 洪惠, 罗永康, 等.冻藏温度对鳙鱼片脂质特性变化的影响[J].中国农业大学学报, 2014, 19 (6) :173-178. [6] 王建辉, 刘冬敏, 刘永乐, 等.冷藏期间草鱼肌肉脂质降解的影响因素分析[J].食品科学, 2013, 34 (18) :276-279. [7] 石雨, 田媛, 李磊, 等.EPA、DHA的生理功能及提取方法的研究进展[J].黑龙江科学, 2014 (10) :24-25. [8] Eunok C, Min D B.Mechanisms of antioxidants in the oxidation of foods[J].Comprehensive Reviews in Food Science and Food Safety, 2009, 8 (4) :345-358.
[9] Carocho M, Ferreira I C.A review on antioxidants, prooxidants and related controversy:natural and synthetic compounds, screening and analysis methodologies and future perspectives[J].Food&Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2013, 51 (1) :15-25.
[10] 张建飞, 朱雪梅, 熊华, 等.α-生育酚在玉米油、大豆油和茶油中的抗氧化动力学研究[J].中国油脂, 2015, 40 (4) :27-32. [11] 王玥, 王兴国, 张亚飞, 等.4种天然抗氧化剂对纯油和人造奶油氧化稳定性的影响[J].食品科学, 2014, 35 (7) :17-22. [12] Liu W, Fu Y, Zu G, et al.Supercritieal carbon dioxide extraction of seed oil from Opuntiadillenii Haw and its antioxidant activity[J].Food Chemistry, 2009, 114 (1) :334-339.
[13] Bou R, Codony R, Baucells M D, et al.Effect of heated sunflower oil and dietary supplements on the composition oxidative stability, and sensory quality of dark chicken meat[J].Journal of Agricultural&Food Chemistry, 2005, 53 (20) :7792-7801.
[14] 孙岩, 王思文, 李铁晶.真空包装烤制鲢鱼贮藏期间的品质研究[J].食品工业科技, 2014, 35 (6) :313-316. [15] Hamre K, Lie O, Sandnes K.Development of lipid oxidation and flesh colour in frozen stored fillets of Norwegian springspawning herring (Clupea harengus L.) :Effects of treatment with ascorbic acid[J].Food Chemistry, 2003, 82 (3) :447-453.
[16] 刘书成, 李元瑞, 王丽华, 等.大蒜精油对食用油脂的抗氧化特性, 2002, 23 (l) :128-131. [17] 王锋, 杨立刚, 孙桂菊, 等.ω-3多不饱和脂肪酸对2型糖尿病患者糖脂代谢的影响[J].食品安全质量检测学报, 2015 (10) :3907-3910. [18] Sperfeld E, Wacker A.Maternal diet of Daphnia magna, affects offspring growth responses to supplementation with particular polyunsaturated fatty acids[J].Hydrobiologia, 2015, 755 (1) :1-16.
[19] 郜海燕, 华颖, 瘦莲, 等.富含不饱和脂肪酸食品加工过程中的组分变化研究与展望[J].中国食品学报, 2011, 11 (9) :134-143.
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