Preparation and antioxidant activity of rice germ Zn2+-polysaccharide
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摘要: 采用超声辅助提取大米胚芽多糖,并以多糖和硫酸锌为原料制备大米胚芽锌多糖络合物并研究其活性。结果表明,大米胚芽锌多糖适宜的制备工艺条件为:多糖与硫酸锌质量比(1.000∶0.800)、溶液p H为6.0、反应温度65℃、反应时间2.5 h。在此条件下,所制备的锌多糖配合物中锌元素含量为(5.617±0.279)mg/g。此外大米胚芽多糖、大米胚芽锌多糖对DPPH自由基清除率为57.76%、62.15%;对超氧阴离子自由基的清除率为60.11%、67.44%;对羟自由基清除率为56.94%、61.89%;对还原力随浓度的增加而增强。可见,大米胚芽锌多糖比大米胚芽多糖的抗氧化活性更强。Abstract: The polysaccharide was extracted from rice germ The polysaccharide complex of rice germ zinc was prepared with polysaccharide and zinc sulfate as raw materials and its activity was studied. The results showed that the optimum preparation conditions were as follows: the mass ratio of polysaccharide to zinc sulfate ( 1.000∶ 0.800) , the p H value of the solution was 6.0, the reaction temperature was 65 ℃, and the reaction time was 2.5 h. Under these conditions, the content of zinc in the zinc polysaccharide complex was ( 5.617 ± 0.279) mg/g. In addition, the scavenging rate of DPPH free radicals was 57.76% and62.15% respectively.The scavenging rate of superoxide anion radical was 60.11% and 67.44% respectively.The scavenging rate of hydroxyl radical was 56.94% and 61.89%.The reduction force increases with increasing concentration.It could be seen that the polysaccharide of rice germ had more antioxidant activity than rice germ polysaccharide.
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