超声辅助提取黑果枸杞花色苷的工艺优化

郑覃; 李建颖; 杨璐; 王青霞; 王佳; 李孟璇

doi:10.13386/j.issn1002-0306.2017.14.044

超声辅助提取黑果枸杞花色苷的工艺优化

天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室

基金项目:

天津商业大学科技成果转化奖励项目(60207-2015CGZH0202)；国家级大学生创新训练计划项目(201510069047)；天津市大学生创业实践计划项目(201610069044)；

详细信息

作者简介:
郑覃 (1994-) , 男, 硕士研究生, 研究方向:食品加工与贮藏, E-mail:m18222069272_1@163.com。;

李建颖 (1961-) , 女, 硕士, 教授, 研究方向:天然产物的提取、分离与纯化, E-mail:ljying@tjcu.edu.cn。;

中图分类号: TQ914.1
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出版历程
- 收稿日期: 2016-11-29

Process optimization for ultrasonic-assisted extraction of anthocyanins from Lycium ruthenicum Murray

College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin Key Laboratory of Food Biotechnology

摘要

摘要: 利用响应面分析法对黑果枸杞花色苷的超声辅助提取工艺进行优化。以总花色苷含量为指标,选取液料比、超声提取温度、超声提取时间和乙醇浓度为主要因素进行单因素实验,并通过响应面分析法进行四因素三水平的Box-Behnken实验,对提取条件进行优化。结果表明:液料比20.4∶1 m L/g,超声提取温度48℃,超声提取时间24.8 min,乙醇浓度79%为最佳提取条件,该条件下花色苷提取量可达（14.999±0.014）mg/g。各因素对花色苷提取量的影响程度依次为:超声提取温度>乙醇浓度>超声提取时间>液料比。
- 黑果枸杞 /
- 响应面分析 /
- 提取 /
- 花色苷 /
- 工艺优化
Abstract: The ultrasonic-assisted extraction process of anthocyanins from Lycium ruthenicum Murray was optimized by response surface methodology in this paper. Total anthocyanins content as index, the liquid to solid ratio, extraction temperature, extraction time and ethanol concentration were selected as the main factors through the single factor experiment.Thus the BoxBehnken experimental design was carried out with four factors and three levels and the response surface methodology was applied to optimize the extraction conditions.The results indicated that the optimal extraction conditions were determined as follows: the liquid to solid ratio of 20.4 ∶ 1 ( m L/g) , extraction temperature of 48 ℃, extraction time of 24.8 min and ethanol concentration of 79%.Under the above conditions, the extraction content of anthocyanins could reach ( 14.999 ± 0.014) mg/g. The primary and secondary order of the factors affecting the extraction content of anthocyanins was extraction temperature, alcohol concentration, extraction time, and liquid to solid ratio.
- Lycium ruthenicum Murray /
- response surface methodology /
- extraction /
- anthocyanins /
- process optimization

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