牡丹花中三种活性成分的快速分离方法

闫慧娇; 王志伟; 王岱杰; 安春燕; 耿岩玲; 王晓

doi:10.13386/j.issn1002-0306.2017.14.037

牡丹花中三种活性成分的快速分离方法

1. 山东省中药质量控制重点实验室山东省分析测试中心
2. 山东理工大学生命科学学院山东省生物信息工程技术中心

基金项目:

国家青年科学基金(21506119)；山东省科学院青年基金项目(青基合字2015第002号)；

详细信息

作者简介:
闫慧娇 (1984-) , 女, 博士, 助理研究员, 主要从事中药学、天然产物化学方面的研究, E-mail:yanhuijiao01@163.com。;

王晓 (1971-) , 男, 博士, 研究员, 研究方向:天然产物化学及中药资源, E-mail:wangx@sdas.org。;

中图分类号: R284.1
计量
- 文章访问数: 0143
- HTML全文浏览量: 022
- PDF下载量: 0235
出版历程
- 收稿日期: 2016-12-11

Rapid isolation of three bioactive components from the flowers of Paeonia suffruticosa Andr.

Shandong Analysis and Test Center, Shandong Key Laboratory Breeding Base of TCM Quality Control Technology
Shandong Provincial Research Center for Bioinformatic Engineering and Technique, School of Life Sciences, Shandong University of Technology

摘要

摘要: 建立应用高速逆流色谱快速分离牡丹花中黄酮及酚酸类活性成分方法。实验采用70%乙醇浸提牡丹花瓣,浸提物减压浓缩后经D101大孔树脂乙醇洗脱富集后,采用乙酸乙酯∶甲醇∶正丁醇∶水=5∶1∶0.5∶4两相系统进行高速逆流色谱分离,化合物结构通过1H NMR、13C NMR、MS确定。从牡丹花中一次性分离出三种化学成分,分别为野漆树苷（Ⅰ）、没食子酸（Ⅱ）、芹菜素-7-O-β-D葡萄糖苷（Ⅲ）,化合物纯度经高效液相色谱检测均达到95%以上。实验建立的方法是快速分离富集纯化牡丹花中三种黄酮、酚酸类活性成分的有效方法。
- 牡丹花 /
- 黄酮 /
- 酚酸 /
- 分离 /
- 高速逆流色谱
Abstract: The present article is to develop new methods for isolation and purification of bioactive components from the flowers of Paeonia suffruticosa Andr.by the combination of macroporous resin column chromatography and high-speed counter-current chromatography.The 70% ethanol extracts of peony flowers was separated by macroporous resin column chromatography and the objective fraction was obtained.Then the fraction was separated by HSCCC with ethyl acetate∶ methanol∶ butyl alcohol∶ water =5∶ 1∶ 0.5∶ 4 two-phase system.The purities of the obtained compounds were determined by HPLC and their chemical structures were identified by1 H NMR, 13 C NMR, MS. Three single compounds, apigenin-7-O-β-D-neospheroside, gallic acid and apigenin-7-O-β-D-glucopyranoside were successfully obtained from Peony flowers and their purities were all over 95%.The developed method was effective in the isolation of flavonoids and phenolic acid from flowers of Paeonia suffruticosa Andr..
- flowers of Paeonia suffruticosa Andr /
- flavonoid /
- phenolic acid /
- isolation /
- high-speed counter-current chromatography

HTML全文

参考文献(15)

[1]	赵兰勇.中国牡丹栽培与鉴赏[M].北京:金盾出版社, 2004:200-280.
[2]	江苏新医学院.中药大辞典上册[M].上海:上海科学技术出版社, 1985:1130.
[3]	闫慧娇, 赵伟, 耿岩玲, 等.牡丹花化学成分研究[J].天然产物研究与开发, 2015, 27 (12) :2056-2059.
[4]	赵伟, 耿岩玲, 崔莉, 等.牡丹花黄酮类化学成分研究[J].中国现代中药, 2016, 18 (3) :303-306.
[5]	史国安, 郭香凤, 包满珠.不同类型牡丹花的营养成分及体外抗氧化活性分析[J].农业机械学报, 2006, 37 (8) :111-114.
[6]	徐小博, 贾文庆, 刘会超.牡丹品种间花瓣营养成分差异[J].浙江农林大学学报, 2012, 29 (5) :729-733.
[7]	张天佑, 王晓.高速逆流色谱[M].北京:化学工业出版社, 2011:1-58.
[8]	曹雪丽.高速逆流色谱分离技术及应用[M].北京:化学工业出版社, 2005:188-189.
[9]	耿岩玲, 刘建华, 王岱杰, 等.超临界CO2萃取-高速逆流色谱分离纯化丹皮中丹皮酚[J].山东科学, 2009, 22 (6) :13-16.
[10]	罗娅君, 边清泉, 张琦, 等.高速逆流色谱分离与鉴定牡荆化学成分[J].分析测试学报, 2011, 30 (9) :1044-1049.
[11]	徐双双, 孙瑜, 井凤, 等.硅胶柱色谱结合高速逆流色谱法分离纯化荷花中黄酮类化合物[J].色谱, 2011, 29 (12) :1244-1248.
[12]	Wang X, Cheng CG, Sun QL, et al.Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography[J].J Chromatogr A, 2005, 1075 (1-2) :127-131.
[13]	章光文, 周国平, 谢二磊, 等.山香圆叶中黄酮类成分研究[J].中国中药杂志, 2009, 34 (12) :1603-1604.
[14]	Sadtler R Lab.Sadtler Standard NMR Spectra[J].Philadelphia:SadtlerRLab Inc, 1971:10229.
[15]	Kubo M, Sasaki H, Endo T, et al.The constituents of Schizonepeta tenuifolia Briq.II Structure of a new monoterpene glucoside, schizonepetoside C[J].Chem Pharm Bull, 1986, 34 (8) :3097-3101.