Effects of selenium enrichment on mycelia dry weight and intracellular enzyme activity of Cordycepsmilitaris
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摘要: 在培养基中添加不同浓度的Na2SeO3液体培养蛹虫草,探究富硒对蛹虫草菌丝干重及胞内酶活性的影响,并进行相关性分析。研究结果表明:当硒添加量为3 mg/kg时,菌丝干重达1.51 g/100 m L,较对照组高19.84%;当硒添加量为7 mg/kg时,菌丝干重较对照组低4.76%,过高的硒添加量会抑制蛹虫草的生长。适宜的硒添加量能在不同程度上提高胞内酶活性,但促进效果存在差异。菌丝干重与蛋白酶活性呈正相关（p<0.01）,R2=0.980,表明蛋白酶是富硒蛹虫草生长中的关键酶。当硒添加量为3 mg/kg时,菌丝干重及蛋白酶活性均达到最大值,可以为富硒蛹虫草液体发酵提供理论依据。Abstract: The effects of selenium enrichment on the mycelia dry weight and intracellular enzyme activity of Cordycepsmilitaris were investigated and the correlation analysis was carried out by adding different concentrations of Na2SeO3 liquid to the culture medium.The results showed that when the selenium content was 3 mg/kg, the dry weight of mycelium was 1.51 g/100 m L, which was 19.84% higher than that of the control.When the selenium content was 7 mg/kg, the dry weight of mycelium was4.76% lower than that of the control.Excessive selenium addition inhibits the growth of Cordycepsmilitaris.The suitable amount of selenium additions could increase the activity of intracellular enzymes in different extent, but the promoting effect was different.At the p < 0.01 level, the dry weight of mycelia was positively correlated with the protease activity, R2= 0.980, indicating that protease was the key enzyme in the growth of Se-enriched Cordycepsmilitaris.When the selenium content was3 mg/kg, the mycelium dry weight and protease activity reached the maximum value, which could provide the theoretical basis for liquid fermentation of selenium-enriched Cordycepsmilitaris.
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