Study on liquid fermentation conditions for fibrinolytic enzyme production by middle acidophilic Actinomycete sp.YY21
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摘要: 为了提高中度嗜酸放线菌YY21产纤溶酶的产量,本文通过单因素实验对其液体发酵培养基和培养条件进行了研究。结果表明:发酵培养基组成为小米粉1.7%,葡萄糖2%,碳酸钙0.2%,氯化钠0.5%,蛋白胨0.6%;发酵条件为250 m L三角瓶装培养基50 m L,接种量5%,在22℃、转速160 r/min的条件下振荡培养4 d。在最适条件下,放线菌YY21液体发酵产物在血纤维蛋白平板上形成溶圈面积可达到164.38 mm~2,较初始产酶活力(溶圈面积87.58 mm~2)提高了88%,发酵时间缩短了3 d。通过对液体发酵培养基和培养条件的研究有效地提高了中度嗜酸放线菌YY21产纤溶酶的产量。
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关键词:
- 中度嗜酸放线菌YY21; /
- 纤溶酶; /
- 液体发酵; /
- 发酵条件; /
Abstract: In the current study, liquid fermentation medium and culture conditions were studied for fibrinolytic enzyme production from middle acidophilic Actinomycete sp.YY21 by single factor experiment.The results showed that the fermentation medium consisted of 1.7% of millet, 2% of glucose, 0.2% of calcium carbonate, 0.5% of sodium chloride and 0.6% of peptone.The optimized fermentation conditions were as follows: 50 m L liquid medium in 250 m L flasks, inoculation volume 5%, rotate speed 160 r/min, culture time 4 d and culture temperature 22 ℃. Under the optimized conditions, the degradation zone area of Actinomycete sp.YY21 product by liquid fermentation reached 164.38 mm2 in the fibrin plate, increasing by 88% than that of original conditions ( 87.58 mm2) .Meanwhile, the fermentation time was decreased by 3 d.The production of fibrinolytic enzyme from middle acidophilic Actinomycete sp. YY21 was enhanced effectively by study of liquid fermentation medium and culture conditions. -
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