Effect of ultrasonic on molecular weight distribution of papain hydrolyzate
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摘要: 本文探讨了超声对木瓜蛋白酶酶解产物分子量大小及分布的影响。以牛血清白蛋白为底物,采用高效液相色谱法测定游离酶和固定化酶在超声和非超声作用下的酶解产物肽分子量大小及分布情况。结果表明,游离酶和固定化酶在超声和非超声条件下,酶解产物肽分子量大小及分布有一定的差异。非超声条件下,游离木瓜蛋白酶酶解效果明显比固定化酶酶解效果要好。比较超声条件下游离木瓜蛋白酶和固定化木瓜蛋白酶酶解产物分子量大小及分布情况发现:游离木瓜蛋白酶超声条件下酶解肽主要分布在180~1000 Da、1000~5000 Da这两个范围内,而固定化木瓜蛋白酶超声条件下酶解肽在<180 Da所占比例比较大。Abstract: The influence of ultrasonic on the molecular weight and distribution of papain hydrolyzate was discussed in this paper.The enzymolysis products of free and immobilized enzymes were determined by high performance liquid chromatography ( HPLC) with bovine serum albumin as substrate.The results showed that there were significant differences in the molecular weight and distribution of the hydrolyzate peptide between free and immobilized enzymes under ultrasound and non-ultrasound conditions.The molecular weight and distribution of free papain and immobilized papain hydrolyzate under ultrasonic conditions were found to be mainly distributed in the range of 1801000 Da and 10005000 Da.The proportion of hydrolyzed peptides in immobilized papain under the condition of < 180 Da was relatively large.
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[1] Moral, Reigm, Toldrf.Bioactive peptides generated from meat industry by-products[J].Food Research International, 2014 (65) :344-349.
[2] 苏秀兰.生物活性肽的研究进展[J].内蒙古医学院学报, 2006, 28 (5) :471. [3] 崔凤霞.海参胶原蛋白生化性质及胶原肽活性研究[D].青岛:中国海洋大学, 2007. [4] 陈星, 李晓磊, 吴琼.不同蛋白酶酶解产物活性大豆肽分子量分布状态的研究[J].西南大学学报 (自然科学版) , 2010 (7) :1-7. [5] 陈季旺, 姚惠源, 陈尚卫.米糠可溶性蛋白酶解物的分子量分布的研究[J].中国油脂, 2004 (1) :36-39. [6] 尹志娜.固定化酶及其在食品和生物领域的研究进展[J].生命科学仪器, 2009, 7 (10) :11-15. [7] Roberto F, Vero’nica R, Cesar M, et al.Stabilization of multiservice enzymes via immobilization and post-immobilization techniques[J].Journal of Molecular Catalysis, 1999 (9) :181-189.
[8] 肖琼, 姚春才, 勇强, 等.玉米秸秆超声辅助酶水解[J].南京林业大学学报, 2007, 31 (4) :85-88. [9] 黄卓烈, 林茹, 何平, 等.超声波对酵母过氧化氢酶及多酚氧化酶活性的影响[J].中国生物工程杂志, 2003, 23 (4) :89-93. [10] Basto C, Silva C J, Gübitz G, et al.Stability and decolourization ability of Trametesvillosa laccase in liquid ultrasonic fields[J].Ultrasonics Sonochemistry, 2007, 14 (3) :355-362.
[11] 黄正华, 曹雁平, 许朵霞, 等.成胶条件对壳聚糖-海藻酸凝胶硬度的影响[J].中国食品学报, 2015 (2) :163-167. [12] 张宜涛, 于伟, 江国永, 等.高效凝胶过滤色谱法测定肽的相对分子量分布[J].饲料畜牧, 2012 (8) :42-44. [13] 张艳艳.基于超声预处理的谷朊蛋白ACE抑制肽制备及其过程原位监测技术研究[D].镇江:江苏大学, 2016. [14] Gtizey D, Giilseren I, Bruce B, et al.Interfacial properties and structural conformation of thermosonicated bovine serum albumin[J].Food Hydrocolloid, 2006 (20) :669-677.
[15] 朱国辉, 黄卓烈, 徐凤彩, 等.超声波对菠萝果蛋白酶活性和光谱的影响[J].应用声学, 2003 (6) :10-14, 38. [16] Haile Ma, Liurong Huang, Junqiang Jia, et al.Effect of energy-gathered ultrasound on Alcalase[J].Ultrasonics Sonochemistry, 2011, 10 (1) :419-424.
[17] 任晓峰.扫频超声预处理对玉米醇溶蛋白特性及其酶法制备ACE抑制肽的影响[D].镇江:江苏大学, 2014. [18] Govind V, Mangesh D, Virendra K.Ultrasound assisted enzyme catalyzed synthesis of glycerol carbonate from glycerol and diethyl carbonate[J].Ultrasonics Sonochemistry, 2015 (22) :311-316.
[19] 朱少娟, 施用晖, 乐国伟.超声波对胰蛋白酶水解酪蛋白的影响[J].食品与生物技术学报, 2005, 24 (2) :50-54. [20] Lin Chen, Jianshe Chen, Jiaoyan Ren.Modications of soy protein isolates using combined extrusion pre-treatment and controlled enzymatic hydrolysis for improved emulsifying properties[J].Food Hydrocolloids, 2011 (25) :887-897.
[21] Z.H Huang, Y P Cao, D X Xu, et al.Effect of ultrasound on the diffusion properties of casein entrapped in alginate-chitosan gel[J].Ultrasonics Sonochemistry, 2015 (26) :149-156.
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