Study on purification and antioxidant activity of D-chiro-inositol from tartary buckwheat
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摘要: 采用超声浸提法提取苦荞D-手性肌醇（DCI）,通过高碘酸钠氧化法测定DCI含量,利用活性炭柱分离纯化苦荞粗提物,高效液相色谱法（HPLC）分析粗提物和纯化产物的组成及含量。以VC、BHT为对照,通过对DPPH自由基、羟自由基、超氧阴离子的体外抗氧化评价体系和总还原力的测定,评价对比苦荞DCI粗提物和纯化产物的抗氧化活性。结果表明,苦荞粗提物中D-手性肌醇的含量仅为0.129%,经活性炭柱纯化后,苦荞中D-手性肌醇的含量可以达到27.26%;苦荞DCI提取物对DPPH自由基、超氧阴离子自由基和羟基自由基均具有不同程度的清除作用,同时具有较好的还原性,且DCI纯化产物的抗氧化活性要明显强于DCI粗提物。Abstract: The D-chiro-inositol was obtained by ultrasonic extraction from tartary buckwheat. The total D-chiro-inositol content was measured by sodium periodate oxidation method.The D-chiro-inositol was purified by activated carbon column.The composition of D-chiro-inositol extractions were analyzed by high performance liquid chromatography ( HPLC) method.Moreover, the reducing power of D-chiro-inositol extractions on DPPH, hydroxyl radicals and superoxide anion free radical in vitro was measured.Results showed that the content of crude D-chiro-inositol extractions in tartary buckwheat only attained0.129%, however, the content of purified buckwheat D-chiro-inositol by actived carbon column could be achieved 27.26%.The antioxidant ability of D-chiro-inositol extractions had higher DPPH free radical, superoxide anion free radical and hydroxyl free radical scavenging effects as well as good reducing capacity.Besides, the antioxidant ability of purified product was better than crude extracting from tartary buckwheat.
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Keywords:
- tartary buckwheat /
- D-chiro-inositol /
- activated carbon /
- HPLC /
- antioxidant activity
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