Comparative research of two omega-3-enriched protein prepared from Antarctic krill ( Euphausia superba) with different methods
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摘要: 以冷冻南极磷虾为原料,通过优化自溶条件,采用控制自溶的方式,制备一种富含ω-3的复合物,并就其基本组成、蛋白质变化与分布、营养评价等,与等电点促溶/促沉法制备的富脂蛋白进行对比研究。优化的自溶条件为55℃和p H7.5条件下自溶3 h。实验结果表明,两种蛋白均为富含ω-3多不饱和脂肪酸的蛋白-脂质复合物,含有全部的人体必需氨基酸,超过FAO/WHO/UNU对食品蛋白质中必需氨基酸的要求,而自溶法制备的富脂蛋白在总脂、磷脂、ω-3多不饱和脂肪酸含量,以及必需氨基酸总量上均优于后者,平均值分别达到了43.88%干基、48.84%总脂、31.52%脂质和517.4 mg/g蛋白。由此可见,控制自溶是一种高效的制备富脂蛋白的方式,有助于磷虾富脂蛋白作为功能性海洋食品的开发。
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关键词:
- 南极磷虾 /
- 富脂蛋白 /
- 自溶 /
- ω-3多不饱和脂肪酸
Abstract: Omega-3-enriched protein ( OEP) was prepared with controlled autolysis from Antarctic krill ( Euphausia superba) meat ( AKM) with optimization, and compared with OEP prepared with isoelectric solubilization/precipitation ( ISP) from basic composition, nutritional evaluation, and variations and distributions of proteins. The optimized condition was that autolysis at55 ℃ and p H7.5 for 3 h, either protein was demonstrated to be a protein-lipid complex which was rich in ω-3 polyunsaturated fatty acids ( ω-3 PUFAs) , and exhibited excellent amino-acid composition in all the essential amino acids, exceeding the requirements of the FAO/WHO/UNU for food protein. Total lipid, phospholipid, ω-3 PUFAs and essential amino acids were richer in the OEP prepared with autolysis, and the average values reached 43.88% dry basis, 48.84% total lipid, 31.52% lipid and 517.4 mg/g protein. This study indicated that controlled autolysis was an efficient way of preparation of ω-3-enriched protein, promotingits development as a functional seafood product in future.-
Keywords:
- Antarctic krill /
- ω-3-enriched protein /
- autolysis /
- ω-3 PUFAs
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