Determination of deoxynivalenols and zearalenone in pastry by solid phase extraction coupled with liquid chromatography-tandem mass spectrometry
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摘要: 建立使用超高效液相色谱-串联质谱仪测定糕点中玉米赤霉烯酮（ZEN）、脱氧雪腐镰刀菌烯醇（DON）及其衍生物3-乙酰基-脱氧雪腐镰刀菌烯醇（3-Ac DON）、15-乙酰基-脱氧雪腐镰刀菌烯醇（15-Ac DON）4种真菌毒素的方法。该方法在4 min内完成4种真菌毒素的分析,线性范围51000μg/kg,标准曲线的相关系数均在0.997以上,DON、ZEN的最低检出限为1.0μg/kg,3-Ac DON、15-Ac DON的最低检出限为1.5μg/kg。通过对134份市售预包装糕点样品进行分析发现,4种真菌毒素均有一定检出,其中ZEN、DON、3-Ac DON和15-Ac DON的检出率分别为2.2%、78.4%、3.0%和5.2%,所有样品中4种真菌毒素含量水平均未超过食品安全国家标准限量要求。
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关键词:
- 固相萃取 /
- 液相色谱-串联质谱仪 /
- 真菌毒素 /
- 糕点
Abstract: A method for simultaneous determination of deoxynivalenol, 3-Acetyl deoxynivalenol, 15-Acetyl deoxynivalenol and zearalenone in pastry had been developed by high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS) .The determination was finished within 4 minutes.The results showed that all of the 4 mycotoxins had good linearity in the range of 51000 μg/kg with the correlation coefficients ( R2) better than 0.997.The limits of detection ( LODs) of DON and ZEN were 1.0 μg/kg, 3-Ac DON and 15-Ac DON were 1.5 μg/kg. The results of 134 pastry samples determined by the method showed that all of the 4 mycotoxins were detected in the tested samples.The detection rate were 2.2%, 78.4%, 3.0% and 5.2%for ZEN, DON, 3-Ac DON and 15-Ac DON. The maximum residual contents were lower than national food safety limited requiements. -
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