高效液相色谱-串联质谱法同时测定枸杞中31种农药残留

汤丽华; 马桂娟; 朱捷; 马雪梅

doi:10.13386/j.issn1002-0306.2017.14.006

高效液相色谱-串联质谱法同时测定枸杞中31种农药残留

宁夏回族自治区食品检测中心

详细信息

作者简介:
汤丽华 (1986-) , 女, 硕士研究生, 工程师, 研究方向:食品分析, E-mail:tanglihua532@163.com。;

中图分类号: O657.63;R284.1
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出版历程
- 收稿日期: 2016-11-22

Determination of 31 pesticide residues in Chinese wolfcherry by high performance liquid chromatography-tandem mass spectrometry

Ningxia Hui Autonomous Region Food Testing Center

摘要

摘要: 建立了高效液相色谱-串联质谱法（HPLC-MS/MS）同时测定枸杞中31种农药残留的方法。枸杞经乙腈提取,Cleant TPH固相萃取柱净化,以C18色谱柱分离待测物,在电喷雾正离子化模式下,采用多反应监测（MRM）检测。结果表明,31种农药在1²00μg/L的范围内线性关系良好,相关系数均大于0.995,方法检出限为0.65⁷.1μg/kg,样品添加回收实验的平均回收率为69.7%¹12.1%,相对标准偏差为5.67%¹4.27%（n=6）。该方法简单准确,适用于枸杞中31种农药残留的同时检测。
- 高效液相色谱-串联质谱法 /
- 枸杞 /
- 农药残留
Abstract: A method for the simultaneous determination of 31 pesticide residues in Chinese wolfcherry by high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS) was developed. The samples were extracted with acetonitrile, cleaned up by Cleant TPH solid phase extraction column. The resulting samples were separated on a C18 column, and detected by multiple reactions monitoring ( MRM) under the condition of positive mode. The results showed that the linear calibration curves were obtained in the entire range ( 1²00 μg/L) for all the above pesticides, with the correlation coefficients higher than 0.995, the detection limit was 0.65 ~ 7.1 μg/kg. For all the samples, the average spiked recoveries ranged 69.7%from 112.1%, and the relative standard deviations ( n = 6) were in the range of 5.67% ~ 14.27%.The method was easy and accurate, and can meet the requirement for the determination of the 31 pesticide residues in Chinese wolfcherry.
- high performance liquid chromatography-tandem mass spectrometry /
- Chinese wolfcherry /
- pesticide residues

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参考文献(10)

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