Determination of diethylstilbestrol in animal food by a poly (tryptophan) modified glassy carbon electrode
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摘要: 用循环伏安法制备了聚色氨酸修饰玻碳电极。研究了己烯雌酚在聚色氨酸修饰电极上的电化学行为,建立了一种直接测定动物性食品中己烯雌酚含量的新方法。实验结果表明:在p H=5.0的磷酸盐缓冲溶液体系中,己烯雌酚在聚色氨酸修饰玻碳电极上于-0.20.8 V范围内出现一对可逆的氧化还原峰,与裸玻碳电极相比较,聚色氨酸修饰电极对己烯雌酚的电化学反应具有促进作用。己烯雌酚浓度在5.00×10-81.00×10-6mol/L范围内与其氧化峰电流成正比,检出限为2.00×10-9mol/L,对己烯雌酚溶液平行测定6次的RSD为2.4%。该修饰电极具有良好的灵敏度、选择性和稳定性,可用于牛肉、鱼肉及奶粉中己烯雌酚含量的测定,回收率为96.0%102.3%,结果满意,方法具有实用价值。Abstract: A poly ( tryptophan) modified glassy carbon electrode ( PTRY/GCE) was prepared by cyclic voltammetry. The electrochemical behavior of diethylstilbestrol ( DES) at the fabricated electrode was studied.A new method for the determination of DES in animal food was established.The experimental results showed that in p H5.0 phosphate buffer solution ( PBS) system, DES appeared a pair of quasi redox peaks in the potential range of-0.2 to 0.8 V on the PTRY/GCE. The modified electrode showed a catalytic effect to the electrochemical reaction of DES.The oxidation peak current was proportional the concentratio of DES with a linear range of 5.00 × 10-8 to 1.00 × 10-6 mol/L and a limit of detection was 2.00 × 10-9mol/L. The RSD for 6paralled determinations was 2.4%.The poly ( tryptophan) modified glassy carbon electrode had good sensitivity, selectivity, and stability.The method could be applied to the determination of DES in beef, fish and milk powder with recoveries of 96.0% 102.3%.The results were satisfactory, and the method had practical value.
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Keywords:
- poly tryptophan /
- modified electrode /
- cyclic voltammetry /
- diethylstilbestrol
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[1] Wang J, Ye H Z, Jiang Z, et al.Detection of diethylstilbestrol by enhancement of luminol-hydrogen peroxide-tetrasulfonated cobalt phthalo-cyanine chemiluminescence[J].Anal Chim Acta, 2004, 508 (2) :171-176.
[2] Liu M H, Li M J, Qiu B, et al.Synthesis and applications of diethylstilbestrol-based molecularly imprinted polymer-coated hollow fiber tube[J].Anal Chim Acta, 2010, 663 (1) :33-38.
[3] 孙延一, 吴康兵, 胡胜水.己烯雌酚在多壁碳纳米管修饰电极上的电化学行为及其电化学测定[J].药学学报, 2003, 38 (5) :364-367. [4] 戚琦, 黄闵, 谢海华.离子液体分散液-液微萃取-高效液相色谱法测定牛奶中己烯雌酚[J].食品科学, 2016, 37 (4) :202-205. [5] Mano M S, Kerr J, Kennedy J.Management of breast cancer in patients prenatally exposed to diethylstilbestrol:are we prepared[J].Breast, 2005, 14 (5) :408-410.
[6] 刘虹, 谢承恩.高效液相色谱法测定奶粉中己烯雌酚[J].应用预防医学, 2006, 12 (6) :374-375. [7] 周建科, 张前莉, 韩康, 等.中老年奶粉中双酚A和己烯雌酚的反相高效液相色谱测定[J].食品工业科技, 2007 (2) :233-234. [8] 程洪兵.高效液相色谱法测定复方己烯雌酚乳膏中己烯雌酚和氯霉素的含量[J].中国药业, 2007, 16 (3) :16-17. [9] 林世蕾, 丁锐, 操基玉.共振散射光谱-免疫分析法测定己烯雌酚[J].环境化学, 2012, 31 (6) :919-920. [10] 孙梅梅, 赵艳伟, 梁艳, 等.荧光免疫法测定鸡肉中己烯雌酚残留[J].理化检验 (化学分册) , 2010, 46 (5) :465-467. [11] 孙俐, 高晓敏.酶联免疫法测定肉及肉制品中己烯雌酚残留量[J].食品研究与开发, 2010, 31 (1) :122-125. [12] Prieto A, Vallejo A, Zuloaga O, et al.Selective detection of estrogenic compounds in water by microextraction by packed sorbents and a molecularly imprinted polymer coupled with large volume injection-in-port-derivatization gas-mass spectrometry[J].Anal Chim Acta, 2011, 703 (1) :41-51.
[13] Quintana J B, Carpinteiro J, Rodríguez I, et al.Detection of natural and synthetic estrogens in water by gas chromatography with mass spectrometric detection[J].J Chromatogr A, 2004, 1024:177-185.
[14] Sanfilippo K, Pinto B, Colombini M P, et al.Detection of trace endocrine disruptors in ultrapure water for laboratory use by the yeast estro-gen screen and chemical analysis (GC/MS) [J].J Chromatogr B, 2010, 878 (15-16) :1190-1194.
[15] 吕昊, 章竹君, 郭志慧, 等.簇状纳米二氧化锰修饰玻碳电极电化学发光法测定己烯雌酚[J].分析化学, 2007, 35 (7) :143-145. [16] Laviron E.General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical system[J].J Electroanal Chem, 1979, 101 (1) :19-28.
[17] Yang D G, Zhu L D, Jiang X Y, et al.Sensitive determination of Sudan I at an ordered mesoporous carbon modified glassy carbon electrode[J].Sensors and Actuators B:Chemical, 2009, 141 (1) :124-129.
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