梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管通透性的保护作用

尚远宏; 田金凤

doi:10.13386/j.issn1002-0306.2017.13.057

梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管通透性的保护作用

攀枝花学院

基金项目:

四川省教育厅重点基金项目(14ZA0345,15ZA0370)；攀枝花市社会发展科技计划项目(2016 CY-S-10)；攀枝花学院博士科研启动经费项目(bkqj2015026,bkqj2015023)；四川省高校重点实验室开放基金项目(GR-2015-E-01,szjj2017-110)；

详细信息

作者简介:
尚远宏 (1980-) , 男, 博士, 讲师, 研究方向:中药药理和食品研究, E-mail:shyh611@126.com。;

中图分类号: R285.5
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出版历程
- 收稿日期: 2016-12-07

Study on the effect of Zige lyophilized powder for injection against the microvascular permeability after cerebral ischemia-reperfusion injury of rats in the celebral microcirculation

Panzhihua University

摘要

摘要: 本文探讨梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管通透性的影响。选择雄性SD大鼠272只,随机分为假手术组、模型组、葛根素注射液组和梓葛冻干粉针3个剂量组。线栓法复制大鼠缺血再灌注模型,分别缺血2 h,于再灌注1、4、7、14 d给药后,取脑提取微血管,用RT-PCR法测定脑微血管中闭合蛋白（Occludin）、紧密连接蛋白-5（Claudin-5）、闭合小带蛋白-1（ZO-1）、基质金属蛋白酶-9（MMP-9）的mRNA表达。结果表明,与模型组相比,再灌注4、7、14 d,梓葛冻干粉针16.40、32.70、65.40 mg·kg^-1能抑制脑缺血/再灌注大鼠缺血侧脑皮质层微血管中MMP-9mRNA表达的升高（p<0.01）;并促进微血管中Claudin-5、Occludin、ZO-1 mRNA表达（p<0.05）。说明梓葛冻干粉针通过抑制MMP-9基因表达,并促进Claudin-5、Occludin、ZO-1基因表达,进而保护和改善脑微血管通透性。
- 梓葛冻干粉针 /
- Claudin-5 /
- Occludin /
- ZO-1 /
- MMP-9 /
- 脑微血管通透性
Abstract: This paper investigated the protective effect of Zige lyophilized powder for injection against the microvascular permeability after cerebral ischemia-reperfusion injury of rats in the celebral microcirculation.272 male sprague-dawley rats were randomly divided into sham group, model group, puerarin injection group, three groups of Zige lyophilized powder.It made a cerebral ischemia-reperfusion model using the line in the left middle cerebral artery occlusion ( MCAO) of rats in ischemia for2 h and reperfusion for 1, 4, 7 and 14 d, the model of rats was established by intravenous injection of drugs. The expression of MMP-9, ZO-1, Occludin and Claudin-5 mRNA of the brain microvessel in the lateral ischemic cerebral cortex was detected by fluorescence quantitative PCR.In reperfusion for 4, 7 and 14 d, compared with model group, Zige lyophilized powder groups ( 16.40, 32.70 and 65.40 mg·kg^-1) could reduce mmp-9 mRNA level in microvessel of the lateral ischemic cerebral cortex ( p < 0.01) , and could increase claudin-5, occludin, zo-1 mRNA level ( p < 0.05) in the corresponding time. These results suggested that Zige lyophilized powder for injection could improve and protect the microvascular permeability by reducing MMP-9 and increasing claudin-5, occludin, zo-1 mRNA level.
- Zige lyophilized powder for injection /
- Claudin-5 /
- Occludin /
- Zo-1 /
- MMP-9 /
- microvascular permeability

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参考文献(17)

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