Abstract: This paper investigated the protective effect of Zige lyophilized powder for injection against the microvascular permeability after cerebral ischemia-reperfusion injury of rats in the celebral microcirculation.272 male sprague-dawley rats were randomly divided into sham group, model group, puerarin injection group, three groups of Zige lyophilized powder.It made a cerebral ischemia-reperfusion model using the line in the left middle cerebral artery occlusion ( MCAO) of rats in ischemia for2 h and reperfusion for 1, 4, 7 and 14 d, the model of rats was established by intravenous injection of drugs. The expression of MMP-9, ZO-1, Occludin and Claudin-5 mRNA of the brain microvessel in the lateral ischemic cerebral cortex was detected by fluorescence quantitative PCR.In reperfusion for 4, 7 and 14 d, compared with model group, Zige lyophilized powder groups ( 16.40, 32.70 and 65.40 mg·kg^-1) could reduce mmp-9 mRNA level in microvessel of the lateral ischemic cerebral cortex ( p < 0.01) , and could increase claudin-5, occludin, zo-1 mRNA level ( p < 0.05) in the corresponding time. These results suggested that Zige lyophilized powder for injection could improve and protect the microvascular permeability by reducing MMP-9 and increasing claudin-5, occludin, zo-1 mRNA level.