Determination of three flavonoids in the leaves of Yunnan Diospyros kaki in different harvest periods by HPLC
-
摘要: 利用回流提取法对不同采收期的云南甜柿叶进行预处理,采用高效液相色谱法测定样品中山奈酚-3-O-β-D-葡萄糖苷、山奈酚-3-O-β-D-半乳糖苷和槲皮素-3-O-β-D-葡萄糖苷的含量。具体方法为:提取条件为提取溶剂45%乙醇,液固比20∶1,提取4次,提取时间90 min;液相色谱条件为采用ZORBAX Eclipse XDB-C18（250 mm×4.6 mm,5μm）色谱柱分离,甲醇-0.4%磷酸溶液为洗脱系统进行梯度洗脱,检测波长265 nm;三种黄酮的相对标准偏差均小于1%,平均回收率均在99%以上。该方法具有较好的分离效果和较高的稳定性,适用于用来分析不同采收期的云南甜柿叶中上述三种黄酮的含量,测定结果表明三种黄酮在叶中积累具有规律性,随柿果实成长不断积累,并且果实成熟时达到最高。
-
关键词:
- 甜柿 /
- 高效液相色谱法 /
- 山奈酚-3-O-β-D-葡萄糖苷 /
- 山奈酚-3-O-β-D-半乳糖苷 /
- 槲皮素-3-O-β-D-葡萄糖苷
Abstract: A HPLC method was developed for determinating the contents of Kaempferol-3-O-β-D-glucopyranoside, Kaempferol-3-O-β-D-galactopyranoside, and Quercetin-3-O-β-D-glucopyranoside in the leaves of Yunnan Diospyros kaki in different harvest periods.The extraction conditions were 45% ethyl alcohol, the ratio of liquid-solid of 20∶ 1, extraction time of 90 min for 4 times.The HPLC elution was carried out using methanol-0.4% phosphate system at a flow rate of 1.0 m L/min, the injection volume was 10 μL, and the detector wavelength was set at 265 nm.The RSD values of the three flavonoids were all less than 1% and the average recoveries were all above 99%.This method showed good separation effect and high stability, and could be used for determining the contents of the three flavonoids in the leaves of Yunnan Diospyros kaki in different harvest periods.The result showed that the accumulation of them in leaves of Yunnan Diospyros kaki exhibited regularity, and the contents were increasing along with fruit growing. -
[1] 王军.牛心柿、橘蜜柿清汁的加工工艺研究[D].西安:陕西师范大学, 2005. [2] 鲁军, 任迪峰, 杨阳, 等.柿叶多酚的提取及体外生物活性的研究[J].食品工业科技, 2010, 31 (7) :88-91. [3] 邓海英, 赖为国.柿叶黄酮对肾性高血压大鼠血管紧张素、醛固酮、降钙素基因相关肽的影响[J].中成药, 2016, 38 (3) :680-682. [4] 韩卫娟, 李加茹, 李华威, 等.不同 (品) 种柿叶总酚与总黄酮含量年动态变化研究[J].中国农业大学学报, 2016, 21 (2) :31-40. [5] 周鑫堂, 王丽莉, 韩璐, 等.柿叶化学成分和药理作用研究进展[J].中草药, 2014, 45 (21) :3195-3203. [6] 卫静莉, 薛笑莉, 陈玉章.提取柿叶黄酮类化合物工艺研究[J].食品研究与开发, 2013, 34 (13) :29-32. [7] 卢鑫, 张琳.柿叶黄酮超声辅助提取工艺及其抗氧化性研究[J].食品研究与开发, 2016, 37 (23) :99-103. [8] 张秀梅, 曾祖平, 王永红.柿叶总黄酮提取工艺的研究[J].时珍国医国药, 2008, 19 (1) :35-36. [9] 韩卫娟, 梁玉琴, 张嘉嘉, 等.柿叶多酚及黄酮类定量分析方法的综述[J].中国农学通报, 2014, 30 (31) :52-56. [10] 罗旭艳, 王丽惠, 王乃平, 等.柿叶总黄酮中槲皮素的含量测定[J].广西医科大学学报, 2011, 28 (1) :26-27. [11] 覃冬杰, 梁永红, 黄瑞松, 等.壮药柿叶槲皮素和山柰素含量测定方法的建立[J].广西医学, 2012, 34 (9) :1133-1136. [12] Amani S A, Maitland D J, Soliman G A.Hepatoprotective activity of Schouwia thebica Webb[J].Bioorganic&Medicinal Chemistry Letters, 2006, 16:4624-4628.
[13] 杨再波, 毛海立, 龙成梅, 等.都匀楼梯草中化学成分研究[J].中国实验方剂学杂志, 2014, 22 (2) :89-91. [14] 贾晓东, 冯煦, 赵兴增, 等.灰毡毛忍冬的化学成分研究[J].中草药, 2008, 39 (11) :1635-1637.
计量
- 文章访问数: 155
- HTML全文浏览量: 26
- PDF下载量: 151
下载:
